Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,033,218 G→T G25V (GGG→GTG)  trkH → potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,033,2180GT95.7% 399.4 / 8.2 115G25V (GGG→GTG) trkHpotassium transporter
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (57/53);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 115 total aligned reads displayed.

CGTGGTTCATTGCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGGGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTG  >  NC_000913_3_pae_tpiA/4033071‑4033363
                                                                                                                                                   |                                                                                                                                                 
cGTGGTTCATTGCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGc                                                                                                                                                 <  2:890763/150‑1 (MQ=255)
cGTGGTTCATTGCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGc                                                                                                                                                 <  3:494664/150‑1 (MQ=255)
  tGGTTCATTGCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTg                                                                                                                                               <  1:921993/150‑1 (MQ=255)
        aTTGCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCa                                                                                                                                         >  1:765039/1‑150 (MQ=255)
           gCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTc                                                                                                                                      >  3:464032/1‑150 (MQ=255)
                    aGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCgg                                                                                                                             <  1:568043/150‑1 (MQ=255)
                       gATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGAt                                                                                                                          >  4:175326/1‑150 (MQ=255)
                            aagaaTAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGc                                                                                                                     >  4:758602/1‑150 (MQ=255)
                              gaaTAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGa                                                                                                                           <  2:674006/142‑1 (MQ=255)
                              gaaTAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGa                                                                                                                           >  1:674006/1‑142 (MQ=255)
                               aataatCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCggg                                                                                                                  <  1:1189466/150‑1 (MQ=255)
                                 taatCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGcc                                                                                                                >  2:473043/1‑150 (MQ=255)
                                       ccACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGGCCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCtt                                                                                                          >  4:440984/1‑150 (MQ=255)
                                       ccACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGGGc                                                                                                                                                 <  3:813157/111‑1 (MQ=255)
                                         aCTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCtttt                                                                                                        <  1:74185/150‑1 (MQ=255)
                                         aCTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCtttt                                                                                                        <  2:455066/150‑1 (MQ=255)
                                          cTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTa                                                                                                       >  3:403739/1‑150 (MQ=255)
                                              gTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCa                                                                                                   <  3:758602/150‑1 (MQ=255)
                                                   gCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCt                                                                                              >  1:766006/1‑150 (MQ=255)
                                                    cAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCtt                                                                                             <  2:550059/150‑1 (MQ=255)
                                                      gAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCtttt                                                                                           <  4:121441/150‑1 (MQ=255)
                                                        aCTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCtttttt                                                                                         <  4:403739/150‑1 (MQ=255)
                                                         cTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCttttttt                                                                                        >  2:779619/1‑149 (MQ=255)
                                                           aaGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTc                                                                                      <  2:710022/150‑1 (MQ=255)
                                                            aGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCg                                                                                     >  3:139558/1‑150 (MQ=255)
                                                             ggAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGc                                                                                    >  2:65727/1‑150 (MQ=255)
                                                              gAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGcc                                                                                   >  2:523556/1‑150 (MQ=255)
                                                              gAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGcc                                                                                   >  2:844670/1‑150 (MQ=255)
                                                                    gCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGcc                                                                             >  4:367890/1‑150 (MQ=255)
                                                                    gCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGcc                                                                             >  3:672627/1‑150 (MQ=255)
                                                                       gagaTGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCAtt                                                                          <  2:518730/150‑1 (MQ=255)
                                                                         gaTGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTgg                                                                        <  1:65727/150‑1 (MQ=255)
                                                                          aTGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCg                                                                                                                              <  1:1136553/95‑1 (MQ=255)
                                                                          aTGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCg                                                                                                                              >  2:1136553/1‑95 (MQ=255)
                                                                           tGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGGCCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGct                                                                      <  3:440984/150‑1 (MQ=255)
                                                                            gCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTg                                                                         <  3:2069/146‑1 (MQ=255)
                                                                            gCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGCACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTg                                                                         >  4:2069/1‑146 (MQ=255)
                                                                                tttCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATg                                                                 <  4:312860/150‑1 (MQ=255)
                                                                                 ttCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATgg                                                                                                                        <  4:688470/94‑1 (MQ=255)
                                                                                 ttCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATgg                                                                                                                        >  3:688470/1‑94 (MQ=255)
                                                                                   cgcgCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCtg                                                              <  1:523556/150‑1 (MQ=255)
                                                                                      gcCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTgtgg                                                           >  1:267271/1‑150 (MQ=255)
                                                                                          ttACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGc                                                       <  3:367890/150‑1 (MQ=255)
                                                                                            aCCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCg                                                     >  1:635618/1‑150 (MQ=255)
                                                                                              ccGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATCCTCTGGTGGCCGaa                                                   >  2:156510/1‑150 (MQ=255)
                                                                                                 aaTCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTAc                                                                                                      <  2:957770/96‑1 (MQ=255)
                                                                                                 aaTCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTAc                                                                                                      >  1:957770/1‑96 (MQ=255)
                                                                                                       tGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTa                                                                                                       >  4:708852/1‑89 (MQ=255)
                                                                                                       tGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTa                                                                                                       <  3:708852/89‑1 (MQ=255)
                                                                                                          actactGGTCATCTTATTTTCAGGGCCCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGaa                                       >  1:550670/1‑150 (MQ=255)
                                                                                                            tactGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTTCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAg                                     >  4:177760/1‑150 (MQ=255)
                                                                                                            tactGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAg                                     >  3:398790/1‑150 (MQ=255)
                                                                                                            tactGGTCATCTTATTCTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAg                                     >  3:752814/1‑150 (MQ=255)
                                                                                                            tactGGTCAACTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAg                                     >  1:942119/1‑150 (MQ=255)
                                                                                                              ctGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGc                                   <  2:68230/150‑1 (MQ=255)
                                                                                                              ctGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGc                                   <  2:94/150‑1 (MQ=255)
                                                                                                               tGGTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCg                                  <  2:765039/150‑1 (MQ=255)
                                                                                                                 gTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATg                                                                                                                         <  1:518016/61‑1 (MQ=255)
                                                                                                                 gTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATg                                                                                                                         >  2:518016/1‑61 (MQ=255)
                                                                                                                 gTCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGACCCGCAAAGAGAAAGGCGaa                                >  1:430551/1‑150 (MQ=255)
                                                                                                                 gTCATCTTATTTTCAGGGACCATGATTATCCCTGGGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGaa                                >  4:777868/1‑150 (MQ=255)
                                                                                                                  tCATCTTATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAAc                               >  3:824890/1‑150 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTAcc                                                                                                                               <  2:963786/49‑1 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTAcc                                                                                                                               >  1:963786/1‑49 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTa                                                                                                       <  2:1162556/73‑1 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTa                                                                                                       >  1:1162556/1‑73 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAg                                                                                                  >  2:943787/1‑78 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAg                                                                                                  <  1:943787/78‑1 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGctc                                                                     <  1:291971/107‑1 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTaaa                          <  3:175326/150‑1 (MQ=255)
                                                                                                                       ttATTTTCAGGGACCATGATTATCCCTGGGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTaaa                          <  3:777868/150‑1 (MQ=255)
                                                                                                                         aTTTTCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATc                        >  3:12499/1‑150 (MQ=255)
                                                                                                                          ttttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGaaa                                      >  4:483603/1‑135 (MQ=255)
                                                                                                                          ttttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGaaa                                      <  3:483603/135‑1 (MQ=255)
                                                                                                                           tttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCtgtg                                                            >  2:1013220/1‑112 (MQ=255)
                                                                                                                           tttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCtgtg                                                            <  1:1013220/112‑1 (MQ=255)
                                                                                                                           tttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATccc                      <  1:822357/150‑1 (MQ=255)
                                                                                                                            ttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAaga                                          >  3:154520/1‑129 (MQ=255)
                                                                                                                            ttCAGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAaga                                          <  4:154520/129‑1 (MQ=255)
                                                                                                                               aGGGACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGa                  >  3:638058/1‑150 (MQ=255)
                                                                                                                                 ggACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATg                                                                 <  1:1206878/101‑1 (MQ=255)
                                                                                                                                 ggACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATg                                                                 >  2:1206878/1‑101 (MQ=255)
                                                                                                                                 atctcATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAgg                <  1:296525/146‑1 (MQ=255)
                                                                                                                                  gACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAg                                                                                                  >  1:80107/1‑67 (MQ=255)
                                                                                                                                  gACCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAg                                                                                                  <  2:80107/67‑1 (MQ=255)
                                                                                                                                   aCCATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAg                                     >  3:280602/1‑127 (MQ=255)
                                                                                                                                    ccATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTgtgg                                                           <  1:1205735/104‑1 (MQ=255)
                                                                                                                                    ccATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTgtgg                                                           >  2:1205735/1‑104 (MQ=255)
                                                                                                                                     cATGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTg                                                                         <  1:821944/89‑1 (MQ=255)
                                                                                                                                       tGATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTc          <  4:398790/150‑1 (MQ=255)
                                                                                                                                        gATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGttt           >  2:236776/1‑148 (MQ=255)
                                                                                                                                        gATTATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGttt           <  1:236776/148‑1 (MQ=255)
                                                                                                                                          ttATCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAAAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGa       <  2:267271/150‑1 (MQ=255)
                                                                                                                                            aTCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCtg                                                              <  2:1218552/93‑1 (MQ=255)
                                                                                                                                            aTCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCtg                                                              >  1:1218552/1‑93 (MQ=255)
                                                                                                                                            aTCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATa     <  2:550670/150‑1 (MQ=255)
                                                                                                                                            aTCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATa     <  4:752814/150‑1 (MQ=255)
                                                                                                                                             tCCCTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAg    >  1:341875/1‑150 (MQ=255)
                                                                                                                                               ccTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAgtg  <  4:464032/150‑1 (MQ=255)
                                                                                                                                               ccTGTGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAgtg  <  4:672627/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTGGTTCATTGCAATTGTTAGCGATTGAAGAATAATCCCCACTTCGTTTTGCAGAACTAAGGAAGCGGCAGAGATGCATTTTCGCGCCATTACCCGAATCGTTGGACTACTGGTCATCTTATTTTCAGGGACCATGATTATCCCTGGGCTGGTAGCACTCATCTACCGGGATGGAGCGGGCCGCGCTTTTACCCAGACCTTTTTTGTCGCCCTCGCCATTGGCTCTATGCTGTGGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTG  >  NC_000913_3_pae_tpiA/4033071‑4033363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: