Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 38 [0] [0] 38 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)38 (0.950) 36/278 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGA  >  NC_000913_3_pae_tpiA/1300555‑1300834
                                                                                                                                               |                                                                                                                                        
aaattacagttagttataacgatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                     >  2:610257‑M2/144‑149 (MQ=255)
     acagttagttataaggatttccttaactgcttctcctcaccattatgttattttcgccacatcataatcctgtgcttgcttaataataatttaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                <  2:534554‑M2/11‑1 (MQ=255)
      cagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                               >  1:372684‑M2/138‑149 (MQ=255)
          gagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                           <  1:399952‑M2/16‑1 (MQ=255)
               ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataataccagtcctgttgaagcatgagtaataggatattattaattccactgcctttggtagaggaaattgctaaATAATAATCAATTTTTAAatt                                                                                                                      >  1:355554‑M2/129‑149 (MQ=255)
                    gaattccttaactgcctctcctcaccaacaatttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatatgattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAATGCTAAACTATtgt                                                                                                                 <  2:7791‑M2/26‑1 (MQ=255)
                      tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGc                                                                                                               >  2:278120‑M2/122‑149 (MQ=255)
                          cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                           >  2:429433‑M2/118‑149 (MQ=255)
                           ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTCTTAAATTATTGTGCATTTc                                                                                                          >  2:292919‑M2/117‑149 (MQ=255)
                            taactgtttctcctcaccataatgatattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                         <  1:482512‑M2/34‑1 (MQ=255)
                              actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCact                                                                                                       >  2:78725‑M2/114‑149 (MQ=255)
                                        ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                             >  1:372289‑M2/104‑149 (MQ=255)
                                           accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTaa                                                                                          <  2:372684‑M2/49‑1 (MQ=255)
                                             catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                        >  2:113801‑M2/99‑149 (MQ=255)
                                                  tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                   >  2:428203‑M2/94‑149 (MQ=255)
                                                       ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAATGTAATCAGAAAAGATa                                                                              >  2:314239‑M2/89‑149 (MQ=255)
                                                        tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                             <  1:429433‑M2/62‑1 (MQ=255)
                                                                                ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttg                                                     >  1:66899‑M2/64‑149 (MQ=255)
                                                                                ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttg                                                     >  2:307094‑M2/64‑149 (MQ=255)
                                                                                      aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGCTAGACTTGCTTAGGCTATCTCTATTTGattgaa                                               <  2:608219‑M2/92‑1 (MQ=255)
                                                                                        gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                             <  1:113801‑M2/94‑1 (MQ=255)
                                                                                                gaaatgatattattaattccactgcctttggtagaggaaagtgctaaACAATAATCAATTGTTCAACTATTGTGCATTTCACTACTAGAACTGTAATCAAAAAGGATAGACATGCTTAGCCAATCTCTATTTCACTGGATTGAAAGAtg                                     <  2:653047‑M2/102‑1 (MQ=255)
                                                                                                   atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                  <  1:253994‑M2/105‑1 (MQ=255)
                                                                                                         ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                            <  1:428203‑M2/111‑1 (MQ=255)
                                                                                                         ttattaattccactgcctttggtagaggaaagtgctaaATAATAAGCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                            <  1:135947‑M2/111‑1 (MQ=255)
                                                                                                          tattaattccactggctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAgg                           <  2:290126‑M2/112‑1 (MQ=255)
                                                                                                           attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                          >  2:629261‑M2/37‑149 (MQ=255)
                                                                                                             taattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                        <  1:78725‑M2/115‑1 (MQ=255)
                                                                                                              aattcccctggctgtggtagcggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                       <  1:317967‑M2/116‑1 (MQ=255)
                                                                                                               attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                             <  2:547632‑M2/110‑1 (MQ=255)
                                                                                                               attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                             >  1:547632‑M2/33‑142 (MQ=255)
                                                                                                                 tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                    <  1:629261‑M2/119‑1 (MQ=255)
                                                                                                                          tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                <  1:133973‑M2/107‑1 (MQ=255)
                                                                                                                          tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                >  2:133973‑M2/22‑128 (MQ=255)
                                                                                                                          tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat           <  1:285910‑M2/128‑1 (MQ=255)
                                                                                                                           ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata          <  1:307094‑M2/129‑1 (MQ=255)
                                                                                                                                 gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt    <  1:278120‑M2/135‑1 (MQ=255)
                                                                                                                                   ggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa  <  2:661983‑M2/137‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                        
GGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGA  >  NC_000913_3_pae_tpiA/1300555‑1300834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.