New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 454260643 (1.040)5 (0.130) 5/268 5.0 10.3% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913_3_pae_tpiA = 4542902 47 (1.210)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGA  >  NC_000913_3_pae_tpiA/4542758‑4543034
                                                                                                                                                |                                                                                                                                    
gttgCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAAAATAGAGAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGc                                                                                                                                  <  1:353391/146‑1 (MQ=255)
          gCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAGTGtt                                                                                                                        <  1:498721/149‑1 (MQ=255)
          gCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGACAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTACTCTATGAGTCAAAATGACCCCAAAGGtt                                                                                                                        >  2:299623/1‑149 (MQ=255)
               ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATc                                                                                                                   >  1:434771/1‑149 (MQ=255)
                   tCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATc                                                                                                                   <  2:368482/145‑1 (MQ=255)
                   tCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATc                                                                                                                   >  1:368482/1‑145 (MQ=255)
                     catcctctctcCTAAAACCTGCGCGTGCAATATAAACATCTAGAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTgg                                                                                                             <  2:462284/144‑1 (MQ=255)
                         ttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGa                                                                                                         >  1:319486/1‑149 (MQ=255)
                         ttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACGGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGCCAAAATGGCCCCAAATGTTTCGTACTTTCGGGGa                                                                                                         >  1:59583/1‑149 (MQ=255)
                                cAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCTCCAAGTGTTTCATGTTTTGGGGGAAAACtgt                                                                                                  >  1:25673/1‑149 (MQ=255)
                                          cTCATGCAGTATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTgg                                                                                        >  1:180754/1‑149 (MQ=255)
                                            cATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCa                                                                                      <  2:382700/149‑1 (MQ=255)
                                              tGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGt                                                                                    >  1:509353/1‑149 (MQ=255)
                                               gCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTc                                                                                   >  2:561241/1‑149 (MQ=255)
                                                cAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTACTTTGCGGGTGTTTAGGGCCCCCAATGTTTCATCTTTTGGGGGAAACCTGTGCAGTGTTGGCGGTCa                                                                                  >  1:169399/1‑149 (MQ=255)
                                                    ataAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACt                                                                              >  1:341481/1‑149 (MQ=255)
                                                        aCATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGtt                                                                          <  2:585151/149‑1 (MQ=255)
                                                             tataAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaa                                                                     >  2:59476/1‑149 (MQ=255)
                                                               taaagaaaGAGAACAGTGGAATATTAAGCCAACATAGAAACTGTAAAAGTTGGTCCGCGTTGCTTTCCTCTATGAGTCAAAATGGTCCCAAATGTTTCATCTTTTGGGGGAAAGCTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaga                                                                   <  2:460218/149‑3 (MQ=255)
                                                                     aaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAgt                                                             <  2:509353/149‑1 (MQ=255)
                                                                        aTAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTTAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTa                                                          <  2:425030/149‑1 (MQ=255)
                                                                                 gAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGAc                                                 >  1:583001/1‑149 (MQ=255)
                                                                                          gCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTAGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAAGGACTGCGCATgc                                        >  1:476127/1‑148 (MQ=255)
                                                                                           ccAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGACAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTc                                       >  1:402028/1‑149 (MQ=255)
                                                                                            caacaaATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCg                                      >  1:448653/1‑149 (MQ=255)
                                                                                              acaaATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGAt                                    >  2:556941/1‑149 (MQ=255)
                                                                                                aaaTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCCTCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGAGTACAGCACGACTGCCCATGTAGAtca                                  >  2:81377/1‑147 (MQ=255)
                                                                                                 aaTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTa                                 <  1:561241/149‑1 (MQ=255)
                                                                                                 aaTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTa                                 <  1:521421/149‑1 (MQ=255)
                                                                                                 aaTAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTa                                 <  1:483198/149‑1 (MQ=255)
                                                                                                  aTAAGCTGAGAAAGTGTGTCCGCGATGTTTTCCGATATGGGTGAAAATGGGCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGGCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAg                                <  2:560499/149‑1 (MQ=255)
                                                                                                  aTAAACTGAAAAAGTTTGTCCGCGATGCTTCCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAg                                <  1:41347/149‑1 (MQ=255)
                                                                                                     aaCTGAAAAAGGTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGaaa                             <  1:211957/149‑1 (MQ=255)
                                                                                                       cTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAGTGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATa                           <  2:684474/149‑1 (MQ=255)
                                                                                                        tGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCACATGTTTCATCTTTTGGGGGGAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATCTCGATTAAGGAATAg                          >  2:333117/1‑149 (MQ=255)
                                                                                                        tGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTCCAAAACAAACTGTACCGAAAGAATGCCCATGCCCTTTCAGAAATAg                          >  2:514806/1‑149 (MQ=255)
                                                                                                          aaaaaGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAACCAATGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGtt                        >  1:201654/1‑149 (MQ=255)
                                                                                                           aaaaGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaac                                                                  >  2:162364/1‑106 (MQ=255)
                                                                                                           aaaaGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaac                                                                  <  1:162364/106‑1 (MQ=255)
                                                                                                              aggttGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATTTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGtttttt                    <  2:363870/146‑1 (MQ=255)
                                                                                                                tttGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCGTCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACTGAACGACTACCCATGTCGATTTAGAAATAGTTTTTTga                  >  2:589885/1‑149 (MQ=255)
                                                                                                                    tCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTgaaagg              >  1:50513/1‑149 (MQ=255)
                                                                                                                      cgcgATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCa                                                                                               >  1:187495/1‑66 (MQ=255)
                                                                                                                      cgcgATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCa                                                                                               <  2:187495/66‑1 (MQ=255)
                                                                                                                       gcgATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGGTTTTTgaaaggaaa           >  1:100509/1‑149 (MQ=255)
                                                                                                                             cTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAgcagca     >  2:540579/1‑149 (MQ=255)
                                                                                                                              tttCCTCTATGAGTCCAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGACGTTTTAGAAATAGTTTTTTGAAAGGAAAGCAGCAt    <  1:176264/149‑1 (MQ=255)
                                                                                                                                tCCTCTAGGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGa  <  2:319486/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                    
GTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGA  >  NC_000913_3_pae_tpiA/4542758‑4543034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.