Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,111,596 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,111,5960GA94.4% 110.9 / NA 36L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (22/12);  minor base T (0/2);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGC  >  NC_000913_3_pae_tpiA/4111450‑4111736
                                                                                                                                                   |                                                                                                                                            
tAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGCAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAcc                                                                                                                                            >  1:27649/1‑149 (MQ=255)
     gAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACGGCGAAGGTCAACCCCttt                                                                                                                                       >  1:223099/1‑149 (MQ=255)
              cgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTg                                                                                                                              >  2:607064/1‑149 (MQ=255)
                   cGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCAAAGGTCAACCCCTTTTCGCCGTTGTcctc                                                                                                                         >  1:619526/1‑147 (MQ=255)
                           agcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACAt                                                                                                                 <  2:348424/149‑1 (MQ=255)
                               ggTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACg                                                                                                             <  2:710946/149‑1 (MQ=255)
                                   tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                                                                                         >  1:688738/1‑149 (MQ=255)
                                     tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGc                                                                                                       >  1:699708/1‑149 (MQ=255)
                                       aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑CTCAGATCGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCTTTGTCCGCGACAACATAACGCAGTgacc                                                                                                     >  2:254840/1‑146 (MQ=255)
                                         aCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCt                                                                                                   >  2:614549/1‑149 (MQ=255)
                                          cAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCtt                                                                                                  <  1:159126/149‑1 (MQ=255)
                                              ataGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGt                                                                                              <  1:481772/149‑1 (MQ=255)
                                                 gagaTTCATTGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtctc                                                                                           >  2:434923/1‑149 (MQ=255)
                                                      tccactgCACATAGGCATTCCTTGTTATCATTTATTTGCTTTTTCAAGCGA‑TTTCGATACCTCCAGCTATTTCGATGGCACCACGCAGGTCGACCCCTTCGCGCCGGTGTCCGCGACAACAGAACGCAGTGCACCTTCTGTATCGgtgt                                                                                      <  2:551388/143‑1 (MQ=255)
                                                         tCGGCACAAAGGCTGTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCTGTGtatt                                                                                   >  2:401219/1‑147 (MQ=255)
                                                            gCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAc                                                                                >  1:508092/1‑149 (MQ=255)
                                                              acaAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgt                                                                              >  1:312967/1‑149 (MQ=255)
                                                                      tttgctttTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGttttt                                                                      >  2:17578/1‑149 (MQ=255)
                                                                              ttGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgccg                                                              <  1:434923/149‑1 (MQ=255)
                                                                                      tATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCACCGCCGTTAGTAGc                                                      >  1:489609/1‑149 (MQ=255)
                                                                                        ttCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCCCCGTTAGGAGCGa                                                    >  1:224682/1‑149 (MQ=255)
                                                                                             aCCTTCAA‑CGACTTCCGTTAGCTCCAGCTAAATCTGTTCAATCGGGAAGGTCATCCACTTTTCGCGGTTGTCCGAGACAACATAACGAAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCa                                               <  2:312967/149‑1 (MQ=255)
                                                                                             aCCCTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCa                                               <  1:451822/149‑1 (MQ=255)
                                                                                               cTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTCATAACGTTTGGTTTTCCCCGCCGTTAGTAGCGACTGCAGt                                             >  1:319331/1‑149 (MQ=255)
                                                                                                cccAAACGA‑TCCAGATCGCGCCAGCTAAATCGGTTCAACAGCTAAGCTCATCCCCTTTTCGCCGTTGTCCGCAAAAACATACCGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtt                                            <  2:508092/147‑1 (MQ=255)
                                                                                                   aaGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                         >  2:390071/1‑149 (MQ=255)
                                                                                                   aaGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACACCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAACTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACGGCAGttttt                                         >  1:603100/1‑149 (MQ=255)
                                                                                                       gA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTg                                     <  1:614549/149‑1 (MQ=255)
                                                                                                        a‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATGCGGTTTGTTTTTCCCCGCCGGTAGTGGGGACTGCCGTGTTTTCAgg                                    >  1:689461/1‑149 (MQ=255)
                                                                                                                tAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgtg                             <  1:607064/149‑1 (MQ=255)
                                                                                                                      cAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTgg                       >  1:614568/1‑149 (MQ=255)
                                                                                                                                 gtgcAACAGCAAAGATCAACCCCGTCTCGCCGGTGTTGGCTACAATAAAACGCTGTGACCCTTCTGTCACGGTGTAATCACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg            <  2:27649/146‑1 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCAACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg            <  1:17578/149‑1 (MQ=255)
                                                                                                                                   ttaaCAGCGAAGGCCAACCCCTATGCGCCATGGTCCGAGATTACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt          <  2:393157/147‑1 (MQ=255)
                                                                                                                                      aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACCACATAACGCAATGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTACCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTgcg       >  2:64997/1‑147 (MQ=255)
                                                                                                                                           gAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc  >  1:377570/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGA‑TTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGC  >  NC_000913_3_pae_tpiA/4111450‑4111736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑