New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 684498 =43 (1.080)5 (0.130) 5/260 4.7 10.6% intergenic (‑86/+32) rihA/gltL ribonucleoside hydrolase 1/glutamate/aspartate ABC transporter ATPase
?NC_000913_3_pae_tpiA 684524 = 44 (1.180)intergenic (‑112/+6) rihA/gltL ribonucleoside hydrolase 1/glutamate/aspartate ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCAT  >  NC_000913_3_pae_tpiA/684379‑684670
                                                                                                                                                 |                                                                                                                                                  
cTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCACTAGAGTATGCGCGCCAGGGt                                                                                                                                                 >  1:92526/1‑149 (MQ=255)
   gatctcAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGAt                                                                                                                                              <  2:529786/144‑1 (MQ=255)
     tCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTa                                                                                                                                            >  1:335011/1‑149 (MQ=255)
         aaTCTAACAGAATTGGCAGTGCCATTGTTGATCATTCGCATGTTCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTagatc                                                                                                                                        >  1:529786/1‑145 (MQ=255)
          aTCTAACAGAAGTGGCAGTGCCATTGGTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTACCGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCa                                                                                                                                       <  1:151791/149‑1 (MQ=255)
          aTCAAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCTGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCa                                                                                                                                       <  2:613940/149‑1 (MQ=255)
           tCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAACTGGGTATGCGCCCCAGAGTGATTaaacaga                                                                                                                                      >  2:178458/1‑144 (MQ=255)
                cAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGAttt                                                                                                                                 >  1:120098/1‑149 (MQ=255)
                cAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAAAGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGCTATGCGGGCAAGAGCGATTAATGACGGAttt                                                                                                                                 >  2:350086/1‑149 (MQ=255)
                 aGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGTTGGCGTGTAATTGGGTATGCGCGCTAGGGTGGTTAATGGTGGAtttt                                                                                                                                >  1:113853/1‑149 (MQ=255)
                  gAAGTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGTTGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTc                                                                                                                               <  1:577949/149‑1 (MQ=255)
                       ggCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCTGCGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAg                                                                                                                          <  1:523350/149‑1 (MQ=255)
                          aGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCCCGCCAGAGGGATTAAGGCAGGAATTTTCCGAGGaa                                                                                                                       >  1:312197/1‑149 (MQ=255)
                                aTTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGAAATTGGGCAGGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCttt                                                                                                                 >  1:521379/1‑149 (MQ=255)
                                           ttgttgTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCg                                                                                                      >  2:589824/1‑149 (MQ=255)
                                                   tgCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGa                                                                                              >  1:320064/1‑149 (MQ=255)
                                                     cttcttTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGatc                                                                                            >  2:153712/1‑149 (MQ=255)
                                                           tGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCgaa                                                                                      <  1:325205/149‑1 (MQ=255)
                                                            gCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCgaag                                                                                     >  1:265545/1‑149 (MQ=255)
                                                                   gggTAACGCCAGGATGGAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGt                                                                              <  2:709155/149‑1 (MQ=255)
                                                                     gTAACGCCAGGATGTAACAGATACGAGGGGCGAACCGATAAAGCGGGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCt                                                                            >  1:701307/1‑149 (MQ=255)
                                                                        aCGCCAGGATGTAACAGATGCGAGGGTGGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTc                                                                         <  2:521379/149‑1 (MQ=255)
                                                                        aCGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGGGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTc                                                                         >  1:495721/1‑149 (MQ=255)
                                                                            cAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATGGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCg                                                                     >  1:386539/1‑149 (MQ=255)
                                                                             aGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGa                                                                    >  1:516693/1‑149 (MQ=255)
                                                                             aGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGa                                                                    <  2:265545/149‑1 (MQ=255)
                                                                              ggATGTAACAGATCCGAGGGGGGAAACGATAAAGCGTGAGATTGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAg                                                                   <  1:222231/149‑1 (MQ=255)
                                                                                     aCAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGa                                                            >  2:603433/1‑149 (MQ=255)
                                                                                         aTACGAGGCGCGAAACGATAAAGCGTGACATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACaac                                                        <  2:113853/149‑2 (MQ=255)
                                                                                             gAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTa                                                    <  1:705094/149‑1 (MQ=255)
                                                                                             gAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTa                                                    >  2:338936/1‑149 (MQ=255)
                                                                                                    gAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGt                                             >  2:442773/1‑149 (MQ=255)
                                                                                                        cGATAAAGCGGGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCAt                                         <  2:701307/149‑1 (MQ=255)
                                                                                                                  tGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCAcc                               <  2:120098/149‑1 (MQ=255)
                                                                                                                  tGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCAcc                               <  1:338936/149‑1 (MQ=255)
                                                                                                                      aTGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCga                                                                                       <  2:368609/89‑1 (MQ=255)
                                                                                                                      aTGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCga                                                                                       >  1:368609/1‑89 (MQ=255)
                                                                                                                       tGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGAtt                          >  2:287748/1‑149 (MQ=255)
                                                                                                                          cgcgcCATTGGGTATGCGCGCCAGGGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGc                       <  1:118516/149‑1 (MQ=255)
                                                                                                                             gcAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATAGAAGCAAGCGCCTTTCGCCGACACTACTGTAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCAc                    <  2:335011/149‑1 (MQ=255)
                                                                                                                               aaTTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCAt                                         >  2:419838/1‑126 (MQ=255)
                                                                                                                               aaTTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCAt                                         <  1:419838/126‑1 (MQ=255)
                                                                                                                               aaTTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACtt                  >  2:234345/1‑149 (MQ=255)
                                                                                                                                 acccaattGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTa                >  1:190656/8‑149 (MQ=255)
                                                                                                                                    ggTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACgg             <  1:287748/149‑1 (MQ=255)
                                                                                                                                              ccAGGGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCa   <  2:92526/149‑1 (MQ=255)
                                                                                                                                               cAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCAt  >  2:150481/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGGGTCGCAATCTAACAGAATTGGCAGTGCCATTGTTGCTCCTTGTTGTGTGCTTCTTTGCGACAAGGGTAACGCCAGGATGTAACAGATACGAGGGGCGAAACGATAAAGCGTGAGATGGCGCGCAATTGGGTATGCGCGCCAGAGTGATTAATGCAGGATTTTCGCGAGGAAGTCTTTTGCGCGGTCCGATTTCGGATCATCGAAGAAAGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCAT  >  NC_000913_3_pae_tpiA/684379‑684670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.