Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 3,431,411 T→C N2S (AAT→AGT)  tsaC ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,431,4110TC81.3% 423.0 / 91.6 193N2S (AAT→AGT) tsaCtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein, threonine‑dependent ADP‑forming ATPase
Reads supporting (aligned to +/- strand):  ref base T (19/17);  new base C (80/77);  total (99/94)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

Only 100 of 195 total aligned reads displayed.

GCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAA  >  NC_000913_3_pae_tpiA/3431262‑3431556
                                                                                                                                                     |                                                                                                                                                 
    ttttAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCAc                                                                                                                                               >  3:506934/1‑150 (MQ=255)
          cTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGttatt                                                                                                                                         <  4:506934/150‑1 (MQ=255)
           tCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGttatta                                                                                                                                        >  1:764273/1‑150 (MQ=255)
               agaGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAGCGGCTTCCGTTGGATAGGCGATTACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTcc                                                                                                                                    <  2:921036/148‑1 (MQ=255)
               aCAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTcc                                                                                                                                    >  1:1394294/1‑150 (MQ=255)
               aCAGGCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTcc                                                                                                                                    <  2:525615/150‑1 (MQ=255)
                 aGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTccgc                                                                                                                                  <  1:998234/150‑1 (MQ=255)
                   tCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAACACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTCTTACTCACGTTATTATTccgccg                                                                                                                                >  3:449666/1‑150 (MQ=255)
                          aCTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCgg                                                                                                                         >  2:2486630/1‑150 (MQ=255)
                            tgctgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCt                                                                                                                       >  1:1224641/1‑150 (MQ=255)
                               tgTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTc                                                                                                                    <  4:258580/150‑1 (MQ=255)
                                  ttCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCcac                                                                                                                 <  1:2470851/150‑1 (MQ=255)
                                       tATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCcaca                                                                                                                <  4:1552471/146‑1 (MQ=255)
                                           aGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTAc                                                                                                        <  4:273828/150‑1 (MQ=255)
                                               tCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGc                                                                                                    >  2:1534850/1‑150 (MQ=255)
                                                     ccAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                              <  2:815856/150‑1 (MQ=255)
                                                     ccAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                              >  2:2084625/1‑150 (MQ=255)
                                                       aaCACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgt                                                                                            >  2:1042731/1‑150 (MQ=255)
                                                         cacCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAgtgt                                                                                          <  3:1524683/150‑1 (MQ=255)
                                                           ccGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGttt                                                                                        >  2:680323/1‑150 (MQ=255)
                                                           ccGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGttt                                                                                        <  3:745922/150‑1 (MQ=255)
                                                            cGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGtttt                                                                                       >  3:1316926/1‑150 (MQ=255)
                                                              aaaaCGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCca                                                                                                                  >  1:841954/1‑121 (MQ=255)
                                                              aaaaCGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGTCACAAAAGTGTTTTac                                                                                     <  3:1420748/150‑1 (MQ=255)
                                                                aaCGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTacac                                                                                   <  2:2476222/150‑1 (MQ=255)
                                                                      ttCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                              >  2:1451793/1‑133 (MQ=255)
                                                                      ttCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                              <  1:1451793/133‑1 (MQ=255)
                                                                        ccGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGcgcg                                                                           <  1:1042731/150‑1 (MQ=255)
                                                                         cGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCgg                                                                          <  1:680323/150‑1 (MQ=255)
                                                                          gTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGt                                                                         >  4:47292/1‑150 (MQ=255)
                                                                           ttGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtt                                                                        >  3:1467606/1‑150 (MQ=255)
                                                                             ggATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTcc                                                                                                                   >  4:1164799/1‑105 (MQ=255)
                                                                             ggATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTcc                                                                                                                   <  3:1164799/105‑1 (MQ=255)
                                                                             ggATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtttt                                                                      <  1:1883138/150‑1 (MQ=255)
                                                                              gATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttc                                                                     >  4:362624/1‑150 (MQ=255)
                                                                              gATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttc                                                                     >  2:1181499/1‑150 (MQ=255)
                                                                                 aGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttcttt                                                                  <  3:682075/150‑1 (MQ=255)
                                                                                  ggCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttctttt                                                                 <  4:761042/150‑1 (MQ=255)
                                                                                  ggCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttctttt                                                                 <  1:2084625/150‑1 (MQ=255)
                                                                                   gCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCcaca                                                                                                                >  2:136731/1‑102 (MQ=255)
                                                                                   gCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtttt                                                                      >  3:315665/1‑144 (MQ=255)
                                                                                   gCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtt                                                                        <  1:525983/142‑1 (MQ=255)
                                                                                   gCGATGACACGTTCTGCCTATAGAACATCTATCGCAGCTGGGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTGTACTGGCACAAGAGTGTTTTTCTCCCTGCGCGGttttcttttc                                                                <  2:58012/150‑1 (MQ=255)
                                                                                     gATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGa                                                              <  2:764273/150‑1 (MQ=255)
                                                                                     gATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGa                                                              <  1:2635537/150‑1 (MQ=255)
                                                                                          cacGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGt                                                         <  1:1534850/150‑1 (MQ=255)
                                                                                           acGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTCGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTa                                                        >  2:2446760/1‑150 (MQ=255)
                                                                                               tcttcATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCgg                                                    <  1:2507710/150‑1 (MQ=255)
                                                                                                    aTTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGa                                                              <  4:1456648/135‑1 (MQ=255)
                                                                                                    aTTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAAt                                               >  2:2233848/1‑150 (MQ=255)
                                                                                                    aTTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAAt                                               <  3:1558948/150‑1 (MQ=255)
                                                                                                     ttGAGAACTTCTATCGCAGCTGCGATAGCCTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTTCACATTGTTTACTTGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATg                                              <  2:2242675/150‑1 (MQ=255)
                                                                                                     ttGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGttt                                                                                        <  4:1178192/108‑1 (MQ=255)
                                                                                                     ttGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATg                                              >  3:846453/1‑150 (MQ=255)
                                                                                                      tGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATg                                              <  3:228747/149‑1 (MQ=255)
                                                                                                      tGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGa                                             <  3:980148/150‑1 (MQ=255)
                                                                                                        agaACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGaca                                           >  3:893902/1‑150 (MQ=255)
                                                                                                          aaCATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACt                                         >  1:1641956/1‑150 (MQ=255)
                                                                                                           aCATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTc                                        <  4:846453/150‑1 (MQ=255)
                                                                                                            acgcTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCg                                                                                                                          <  4:1376516/64‑1 (MQ=39)
                                                                                                             aTCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTAc                                                                                                        <  2:982019/84‑1 (MQ=255)
                                                                                                             aTCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAg                                      >  3:909632/1‑150 (MQ=255)
                                                                                                                 aTCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCg                                                                                                                          >  4:227467/1‑62 (MQ=255)
                                                                                                                  tCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                              <  1:2002129/89‑1 (MQ=255)
                                                                                                                  tCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACaaaa                                                                                              >  2:2002129/1‑89 (MQ=255)
                                                                                                                  tCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttctttt                                                                 <  2:1651563/118‑1 (MQ=255)
                                                                                                                  tCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtt                                                                        >  3:1197923/1‑111 (MQ=255)
                                                                                                                    gCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATtc                               >  4:1198911/1‑150 (MQ=255)
                                                                                                                       gCTGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTcc                            >  1:471210/1‑150 (MQ=255)
                                                                                                                         tGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAg                          <  4:893902/150‑1 (MQ=255)
                                                                                                                         tGCGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGa                                                              >  2:1863920/1‑114 (MQ=255)
                                                                                                                           cGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGa                                             >  2:329841/1‑129 (MQ=255)
                                                                                                                           cGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGa                                             <  1:329841/129‑1 (MQ=255)
                                                                                                                           cGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACAcc                                                                                  <  4:581297/92‑1 (MQ=255)
                                                                                                                           cGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACAcc                                                                                  >  3:581297/1‑92 (MQ=255)
                                                                                                                           cGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAgg                                     <  3:1096277/137‑1 (MQ=255)
                                                                                                                           cGATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAgg                                     >  4:1096277/1‑137 (MQ=255)
                                                                                                                            gATAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTa                       >  4:445368/1‑150 (MQ=255)
                                                                                                                              tAGCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGAGCGGTTTTTTTTTCGATGAGTAGCGGATACTGACACTCAGGGCATTCTCCAGCTATg                     >  1:1061628/1‑150 (MQ=255)
                                                                                                                                gCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATggg                   <  4:414616/150‑1 (MQ=255)
                                                                                                                                gCGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATggg                   >  2:2312061/1‑150 (MQ=255)
                                                                                                                                 cGTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAACGACACTCAGGGCATTCTCCAGCTATGGGtt                 >  2:475731/1‑150 (MQ=255)
                                                                                                                                  gTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATtctc                             <  2:454540/138‑1 (MQ=255)
                                                                                                                                  gTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATtctc                             >  1:454540/1‑138 (MQ=255)
                                                                                                                                  gTCTCTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGtt                 >  2:951162/1‑150 (MQ=255)
                                                                                                                                   tctcTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGtt                                                                                                           <  3:1052223/59‑1 (MQ=255)
                                                                                                                                   tctcTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGtt                                                                                                           >  4:1052223/1‑59 (MQ=255)
                                                                                                                                   tctcTTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGttt                <  1:2280394/150‑1 (MQ=255)
                                                                                                                                     tctTTGCAGGTTATTACTGACGTTATTATTCCGCCGAAACCGGCTTTGCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttcttt                                                                  >  2:1639292/1‑98 (MQ=255)
                                                                                                                                     tctTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTac                                                                                     <  1:1727835/79‑1 (MQ=255)
                                                                                                                                     tctTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGa              <  1:1181499/150‑1 (MQ=255)
                                                                                                                                     tctTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGa              <  1:2312061/150‑1 (MQ=255)
                                                                                                                                      ctTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGaa             <  3:1198911/150‑1 (MQ=255)
                                                                                                                                      ctTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGAt                                                  >  1:1637961/1‑113 (MQ=255)
                                                                                                                                      ctTTGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGaa             <  1:952883/150‑1 (MQ=255)
                                                                                                                                       tttGCAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGtttt                                                                      >  2:1595465/1‑92 (MQ=255)
                                                                                                                                           cAGGTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTaa        <  4:909632/150‑1 (MQ=255)
                                                                                                                                              gTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGttttctt                                                                   <  4:699639/88‑1 (MQ=255)
                                                                                                                                              gTTATTACTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTGGACACCCCGCGCGGGTTGCTGTTCGAAGAGTAGCGGATAAGGACACTCAGGGCAGTCTCCAGCTATGGGTTTGAAGTTAATgg     >  1:1330217/1‑150 (MQ=255)
                                                                                                                                                 attactCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGcgt  >  1:969855/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCTGTTTTAACTCCAACAGTCGCATCACTGCTGTTTCGCTATCAGGATCGCACCCAACACCGAAAACGGCTTCCGTTGGATAGGCGATGACACGTTCTTCATTGAGAACATCTATCGCAGCTGCGATAGCGTCTCTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAA  >  NC_000913_3_pae_tpiA/3431262‑3431556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: