Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,756,356	A→C	K220T (AAA→ACA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,756,356	0	A	C	98.7%	263.7 / NA	75	K220T (AAA→ACA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (39/35);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAAT  >  NC_000913_3_pae_tpiA/1756209‑1756496                                                                                                                                                    |                                                                                                                                             cTGAAAAAGACTAACAGGACCTGCTCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCacaa                                                                                                                                            <  2:94803/150‑1 (MQ=255)   gAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATc                                                                                                                                          <  4:479229/150‑1 (MQ=255)      aaaGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGaa                                                                                                                                       <  4:17612/150‑1 (MQ=255)        aGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAAACCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGaaaa                                                                                                                                     <  2:682286/150‑1 (MQ=255)         gACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAAc                                                                                                                                    <  2:228692/150‑1 (MQ=255)           cAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCa                                                                                                                                  <  3:433178/150‑1 (MQ=255)           cAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCa                                                                                                                                  <  2:592912/150‑1 (MQ=255)            aaaCAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAg                                                                                                                                 >  1:837866/1‑150 (MQ=255)                 ggACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATAAAAAAAAAAAAAAACCAGGAAgg                                                                                                                            <  4:355296/150‑1 (MQ=255)                  gACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAGCATCCACATCATCTTCACAATCGAAAACCAg                                                                                                                                 >  1:501254/1‑144 (MQ=255)                  gACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTTCACAATCGAAAACCAg                                                                                                                                 <  2:501254/144‑1 (MQ=255)                  gACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGc                                                                                                                           >  4:328766/1‑150 (MQ=255)                     cTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTc                                                                                                                        <  1:418978/150‑1 (MQ=255)                             tGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACtt                                                                                                                <  1:616531/150‑1 (MQ=255)                                 tGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTcgac                                                                                                            <  1:531651/150‑1 (MQ=255)                                        aaGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATcc                                                                                                     <  4:594386/150‑1 (MQ=255)                                          ggCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTc                                                                                                   <  3:83767/150‑1 (MQ=255)                                           gCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAgg                                                                                                                            <  3:32906/124‑1 (MQ=255)                                           gCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAgg                                                                                                                            >  4:32906/1‑124 (MQ=255)                                             gTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGaa                                                                                                >  3:418717/1‑150 (MQ=255)                                              tAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTc                                                                                                                        >  4:196169/1‑125 (MQ=255)                                              tAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTc                                                                                                                        <  3:196169/125‑1 (MQ=255)                                                          ctgctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGca                                                                                   <  4:78206/150‑1 (MQ=255)                                                             ctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGcatca                                                                                >  4:243734/1‑150 (MQ=255)                                                               tCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATg                                                                              >  2:479484/1‑150 (MQ=255)                                                                 cTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCCCGAAGCCTCTGACGGCATCATGGt                                                                            >  4:368376/1‑150 (MQ=255)                                                                    tATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGcc                                                                                                                          <  3:246036/101‑1 (MQ=255)                                                                    tATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGcc                                                                                                                          >  4:246036/1‑101 (MQ=255)                                                                       tCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGtcgccac                                                                      >  1:661664/1‑144 (MQ=255)                                                                          tAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTgg                                                                   >  2:837461/1‑150 (MQ=255)                                                                           aaGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGc                                                                  >  3:355136/1‑150 (MQ=255)                                                                             gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTgg                                                                   >  2:239336/1‑147 (MQ=255)                                                                             gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTgg                                                                   <  1:239336/147‑1 (MQ=255)                                                                             gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTg                                                                    <  3:84111/146‑1 (MQ=255)                                                                             gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTg                                                                    >  4:84111/1‑146 (MQ=255)                                                                             gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGa                                                                >  1:80633/1‑150 (MQ=255)                                                                             gCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGa                                                                >  1:343283/1‑150 (MQ=255)                                                                                 tCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTg                                                            <  4:628/150‑1 (MQ=255)                                                                                  cTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTgg                                                           <  4:493370/150‑1 (MQ=255)                                                                                   tGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTggg                                                          >  2:769405/1‑150 (MQ=255)                                                                                   tGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTggg                                                          >  2:34299/1‑150 (MQ=255)                                                                                    gACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGgt                                                         >  2:601767/1‑150 (MQ=255)                                                                                     aCGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGt                                                                     >  4:365058/1‑137 (MQ=255)                                                                                     aCGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGt                                                                     <  3:365058/137‑1 (MQ=255)                                                                                     aCGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGgtg                                                        <  1:302137/150‑1 (MQ=255)                                                                                       gTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGaa                                                                                                                              >  4:24568/1‑78 (MQ=255)                                                                                       gTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGaa                                                                                                                              <  3:24568/78‑1 (MQ=255)                                                                                         tATCGACATCCGTGAGCACCGGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAACTCCTCGAAGCCTCTGACGGCATCATGGTTGCTCGTGCCGACCTGGGTGCAGa                                                    >  4:551879/1‑150 (MQ=255)                                                                                         tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGa                                                    >  3:440040/1‑150 (MQ=255)                                                                                          aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGaa                                                   >  2:702450/1‑150 (MQ=255)                                                                                           tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGaaa                                                  >  3:512330/1‑150 (MQ=255)                                                                                            cGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                                 >  2:206911/1‑150 (MQ=255)                                                                                             gAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATc                                                >  1:559920/1‑150 (MQ=255)                                                                                              aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATcc                                               >  1:428734/1‑150 (MQ=255)                                                                                                  ccGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGt                                           <  2:428734/150‑1 (MQ=255)                                                                                                         cACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAgaagaa                                    <  4:355136/150‑1 (MQ=255)                                                                                                          aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTcga                                                                                                             >  2:493509/1‑76 (MQ=255)                                                                                                          aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTcga                                                                                                             <  1:493509/76‑1 (MQ=255)                                                                                                          aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTa                                                      >  1:920452/1‑131 (MQ=255)                                                                                                          aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTa                                                      <  2:920452/131‑1 (MQ=255)                                                                                                          aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGaagaag                                   >  1:114268/1‑150 (MQ=255)                                                                                                            cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAGTCGAAAACCTGGAAGTCCTCAACAACTTCGACGAAATCCGCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGtt                                 >  3:391547/1‑150 (MQ=255)                                                                                                            cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGt                                           >  2:120915/1‑140 (MQ=255)                                                                                                            cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGt                                           <  1:120915/140‑1 (MQ=255)                                                                                                            cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGtt                                 <  1:212340/150‑1 (MQ=255)                                                                                                             tGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTa                                >  1:187256/1‑150 (MQ=255)                                                                                                             tGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTa                                <  3:243734/150‑1 (MQ=255)                                                                                                                 aGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                                     <  4:362396/93‑1 (MQ=255)                                                                                                                 aGCGCACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                                     >  3:362396/1‑93 (MQ=255)                                                                                                                  gcgcACGGCGGCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                           <  3:600690/150‑1 (MQ=255)                                                                                                                       cggcggCGAAAACATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTGGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCa                      >  1:570972/1‑150 (MQ=255)                                                                                                                               aaaaCATCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga              <  1:702450/150‑1 (MQ=255)                                                                                                                                    aTCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGACGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATc         >  1:836714/1‑150 (MQ=255)                                                                                                                                    aTCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATc         <  1:837461/150‑1 (MQ=255)                                                                                                                                     tCCACATCATCTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCATCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCg        <  3:328766/150‑1 (MQ=255)                                                                                                                                           tcatcTCCACAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAAt  >  2:965594/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                             CTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAAT  >  NC_000913_3_pae_tpiA/1756209‑1756496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑