Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,034,189	T→G	S349A (TCA→GCA)	trkH →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,034,189	0	T	G	100.0%	250.9 / NA	72	S349A (TCA→GCA)	trkH	potassium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (32/40);  total (32/40)

CGCTGATGACAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGA  >  NC_000913_3_pae_tpiA/4034046‑4034328                                                                                                                                                |                                                                                                                                            cgcTGATGACAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCCCCGGGGCAACgg                                                                                                                                       >  1:129256/1‑150 (MQ=255)   cTGATGACAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACACCCGCCGGGTTTACAACTGACAGCTTTGCCCGCTTGCAGCTCTTTATGGCGGTACTGCTTTTATGTTCCGCCTTTATGGGCGGTTGCGCCGGGGCAACGGGc                                                                                                                                     <  4:363626/150‑1 (MQ=255)      atgaCAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGt                                                                                                                                  >  2:907809/1‑150 (MQ=255)       tgaCAATTAACCAGGCGTTTTTCCAGGTGTTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTg                                                                                                                                 <  2:618315/150‑1 (MQ=255)       tgaCAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCTCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTg                                                                                                                                 <  3:2034/150‑1 (MQ=255)       tgaCAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCACCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTg                                                                                                                                 <  4:296677/150‑1 (MQ=255)        gaCAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTgg                                                                                                                                <  2:192611/150‑1 (MQ=255)         aCAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGc                                                                                                                               <  1:393691/150‑1 (MQ=255)              tAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCTCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGaa                                                                                                                          <  3:75139/150‑1 (MQ=255)               aaacAGGCGGTGTTCCAGGGGGTGTCGGTGGCGGCAACCGCCGGGTTTACAACTGACAGCCTTGCCCGCTGGCCGGTCTTTTTGCCGGTACTGCTTTTATGGTCAGCCCTTAACGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAg                                                                                                                         <  2:129256/147‑1 (MQ=255)                     gCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATc                                                                                                                   <  4:221627/150‑1 (MQ=255)                      cGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATcc                                                                                                                  >  3:590331/1‑150 (MQ=255)                             ccAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCct                                                                                                           <  2:968543/150‑1 (MQ=255)                              cAGGTGGTGTCGGTGGCGACGACCGCCGGGTTTGCAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctg                                                                                                          <  2:107714/150‑1 (MQ=255)                              cAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctg                                                                                                          <  1:479560/150‑1 (MQ=255)                                 gtggtgTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctgctg                                                                                                       >  1:720615/1‑150 (MQ=255)                                   ggtgTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctgctgct                                                                                                     <  3:458911/150‑1 (MQ=255)                                    gtgtCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctgctgctg                                                                                                    >  1:5244/1‑150 (MQ=255)                                      gtCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCg                                                                                                                                    <  4:372257/116‑1 (MQ=255)                                      gtCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCg                                                                                                                                    >  3:372257/1‑116 (MQ=255)                                      gtCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCg                                                                                                                                    <  3:400314/116‑1 (MQ=255)                                      gtCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCg                                                                                                                                    >  4:400314/1‑116 (MQ=255)                                      gtCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGtt                                                                                                  <  3:411558/150‑1 (MQ=255)                                           tGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGc                                                                                             >  2:829693/1‑150 (MQ=255)                                            ggCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTaa                                                                                               <  1:498684/147‑1 (MQ=255)                                            ggCGACAACCGCCGGGATTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTaa                                                                                               >  2:498684/1‑147 (MQ=255)                                              cGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAgg                                                                                          <  2:42851/150‑1 (MQ=255)                                               gACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAggg                                                                                         >  1:273346/1‑150 (MQ=255)                                                      gccgGGTTTACAACTGACATCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGt                                                                                  <  1:696113/150‑1 (MQ=255)                                                      gccgGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGt                                                                                  >  1:162631/1‑150 (MQ=255)                                                            tttACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTg                                                                            <  4:590331/150‑1 (MQ=255)                                                                    tGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACt                                                                    >  1:57151/1‑150 (MQ=255)                                                                       cAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGt                                                                 <  2:5244/150‑1 (MQ=255)                                                                       cAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGt                                                                 <  2:748604/150‑1 (MQ=255)                                                                        aGCATTGCCCGCTGGCCGCTCTTTTTGACGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGAGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTg                                                                >  2:104571/1‑150 (MQ=255)                                                                              gCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCg                                                          <  4:584759/150‑1 (MQ=255)                                                                              gCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCg                                                          <  4:584767/150‑1 (MQ=255)                                                                               cccGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGa                                                         >  1:524150/1‑150 (MQ=255)                                                                                 cGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCCTCCGAAc                                                       <  2:971478/150‑1 (MQ=255)                                                                                    tGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGcc                                                    <  2:664389/150‑1 (MQ=255)                                                                                        cGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCt                                                <  2:273346/150‑1 (MQ=255)                                                                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTTCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCgata                                             >  1:320110/1‑150 (MQ=255)                                                                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGa                                                                      >  4:208160/1‑125 (MQ=255)                                                                                           tctTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGa                                                                      <  3:208160/125‑1 (MQ=255)                                                                                              ttttGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCa                                          >  4:283913/1‑150 (MQ=255)                                                                                                   ccGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGCGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAg                                     >  4:561857/1‑150 (MQ=255)                                                                                                      gTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTg                                  >  1:531343/1‑150 (MQ=255)                                                                                                      gTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTg                                  >  2:409940/1‑150 (MQ=255)                                                                                                        aCTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTggg                                <  1:409940/150‑1 (MQ=255)                                                                                                        aCTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTggg                                <  1:857942/150‑1 (MQ=255)                                                                                                            ctgttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctg                                                                                                          <  1:877590/69‑1 (MQ=255)                                                                                                            cTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCctg                                                                                                          >  2:877590/1‑72 (MQ=255)                                                                                                             ttttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCtat                                              <  2:120134/131‑1 (MQ=255)                                                                                                             ttttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCtat                                              >  1:120134/1‑131 (MQ=255)                                                                                                             ttttATGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATc                           >  3:535645/1‑150 (MQ=255)                                                                                                                  tGTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATc                                                                                                             <  2:284103/63‑1 (MQ=255)                                                                                                                  ggTTCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATc                                                                                                             >  1:284103/2‑63 (MQ=255)                                                                                                                    ttCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACt                    >  4:115862/1‑150 (MQ=255)                                                                                                                    ttCAGCCTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGGCTATAGCATTAAGCCGGGGAATCGCGCACt                    >  2:329740/1‑150 (MQ=255)                                                                                                                         ccTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCgg               >  1:746525/1‑150 (MQ=255)                                                                                                                          cTTTATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGa              >  1:788322/1‑150 (MQ=255)                                                                                                                           tttATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGaa             >  3:101370/1‑150 (MQ=255)                                                                                                                            ttATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAAc            <  2:57151/150‑1 (MQ=255)                                                                                                                            ttATCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAAc            <  4:586579/150‑1 (MQ=255)                                                                                                                              aTCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCCGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGt          <  1:711872/150‑1 (MQ=255)                                                                                                                               tCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGcc                                                    >  1:835699/1‑107 (MQ=255)                                                                                                                               tCGGCGGTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGcc                                                    <  2:835699/107‑1 (MQ=255)                                                                                                                                    ggTTGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGTAACCGTGAGCTGAAATGCCTGGTGCATCCGAACGCCGTCTATATCATTAAGCTGGGGATTCGCGCACTGCCGGAACGTATCCTc    <  4:475958/150‑1 (MQ=255)                                                                                                                                     gTTGCGCCGGGGCAACGGGCGGGGGGCTGAAGGTGATCCGCATTCTGCTGCTGTTTAAGCAGGGGAACAGTGAGCTGAAACGACTGGTGCAACCGAACGCCGTCTATCGCATTGAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCg   >  4:4766/1‑150 (MQ=255)                                                                                                                                      ttGCGCCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGa  <  1:907809/150‑1 (MQ=255)                                                                                                                                          gcCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGa                                                                      <  1:170911/78‑1 (MQ=255)                                                                                                                                          gcCGGGGCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGa                                                                      >  2:170911/1‑78 (MQ=255)                                                                                                                                                |                                                                                                                                            CGCTGATGACAATTAACCAGGCGTTTTTCCAGGTGGTGTCGATGGCGACAACCGCCGGGTTTACAACTGACAGCATTGCCCGCTGGCCGCTCTTTTTGCCGGTACTGCTTTTATGTTCAGCCTTTATCGGCGGTTGCGCCGGGTCAACGGGCGGTGGCCTGAAGGTGATCCGCATCCTGCTGCTGTTTAAGCAGGGGAACCGTGAGCTGAAACGACTGGTGCATCCGAACGCCGTCTATAGCATTAAGCTGGGGAATCGCGCACTGCCGGAACGTATCCTCGA  >  NC_000913_3_pae_tpiA/4034046‑4034328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑