Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,158,703	A→G	T100A (ACA→GCA)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,158,703	0	A	G	100.0%	247.9 / NA	70	T100A (ACA→GCA)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/40);  total (30/40)

CAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTG  >  NC_000913_3_pae_tpiA/4158558‑4158849                                                                                                                                                  |                                                                                                                                                   cAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGt                                                                                                                                                <  1:228402/150‑1 (MQ=255)     cGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGa                                                                                                                                            <  4:293191/150‑1 (MQ=255)          aGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTa                                                                                                                                       <  2:899335/150‑1 (MQ=255)              ttgttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTg                                                                                                                                   <  1:261420/150‑1 (MQ=255)               tgttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGc                                                                                                                                  <  3:141700/150‑1 (MQ=255)                    aCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGCCCGTACCTGCTACCg                                                                                                                             <  4:497342/150‑1 (MQ=255)                        aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGt                                                                                                                                        <  2:874031/135‑1 (MQ=255)                        aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGt                                                                                                                                        >  1:874031/1‑135 (MQ=255)                           cGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtatta                                                                                                                      <  4:119118/150‑1 (MQ=255)                            gggAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCAAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                                     <  4:456296/150‑1 (MQ=255)                               aaTGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATccc                                                                                                                  <  3:420769/150‑1 (MQ=255)                                   ctgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtg                                                                                                              >  3:388182/1‑150 (MQ=255)                                    tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGAAGTCCGGACCGCTGCACATTGGTTAGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                                                                             >  2:666095/1‑150 (MQ=255)                                       tggtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                          >  3:61344/1‑150 (MQ=255)                                       tggtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                          >  4:12252/1‑150 (MQ=255)                                        ggtggATCAGGCGCGTACCGTGCTGCGTGAGCTTAATTGCCCTAATGAGATGTCAAACCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCa                                                                                                         <  1:923834/150‑1 (MQ=255)                                         gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                        >  3:433122/1‑150 (MQ=255)                                         gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                        >  2:670932/1‑150 (MQ=255)                                              tCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGAc                                                                                                   >  1:961290/1‑150 (MQ=255)                                                aGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCt                                                                                                 >  1:589702/1‑150 (MQ=255)                                                  gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCCCATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               <  4:476437/150‑1 (MQ=255)                                                  gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               >  4:491325/1‑150 (MQ=255)                                                           gTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTACGGAACGCTGAACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTg                                                                                      <  3:422102/150‑1 (MQ=255)                                                                gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCCATGCTGCACCAGACCTTTCCAAAGCTGGAAAt                                                                                 <  3:387634/150‑1 (MQ=255)                                                                    gAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGATACCGCGTATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                             <  4:361038/150‑1 (MQ=255)                                                                       gTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTg                                                                          <  2:844841/150‑1 (MQ=255)                                                                          aaaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                       >  4:306521/1‑150 (MQ=255)                                                                           aaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                             <  2:972312/143‑1 (MQ=255)                                                                           aaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                             >  1:972312/1‑143 (MQ=255)                                                                            aGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGa                                                                     <  4:593912/150‑1 (MQ=255)                                                                             gTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGaa                                                                    >  4:341023/1‑150 (MQ=255)                                                                              tCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                                     >  2:552861/1‑100 (MQ=255)                                                                              tCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                                     <  1:552861/100‑1 (MQ=255)                                                                               ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCa                                                                                                                           <  1:222863/93‑1 (MQ=255)                                                                               ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCa                                                                                                                           >  2:222863/1‑93 (MQ=255)                                                                                 ttAAAGAGATGGCAAGCCCGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCGCTCGGCCCGTACCTGCGACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                <  1:362283/150‑1 (MQ=255)                                                                                 ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                >  2:221268/1‑150 (MQ=255)                                                                                 ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                >  1:323764/1‑150 (MQ=255)                                                                                  tAAAGAGATGGCAAGCCAGCAGGGCGGGACTTCGCCCGGCCCGCTGTTCAGGCGGCCGATGGCGGTTTGCGGACCGTACCGGCTACCTCATATGATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcaca                                                               <  1:877695/150‑1 (MQ=255)                                                                                   aaaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAg                                                              >  2:493963/1‑150 (MQ=255)                                                                                       agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTgg                                                                                                                                             <  1:416512/67‑1 (MQ=255)                                                                                       agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTgg                                                                                                                                             >  2:416512/1‑67 (MQ=255)                                                                                              aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTg                                                                          <  2:927500/127‑1 (MQ=255)                                                                                              aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTg                                                                          >  1:927500/1‑127 (MQ=255)                                                                                              aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGtt                                                   >  2:390316/1‑150 (MQ=255)                                                                                                gCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTAc                                                 <  1:897359/150‑1 (MQ=255)                                                                                                      agGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgcgc                                           <  4:429642/150‑1 (MQ=255)                                                                                                         gCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCGCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAAc                                        >  2:370367/1‑150 (MQ=255)                                                                                                         gCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAAc                                        <  4:61344/150‑1 (MQ=255)                                                                                                         gCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAAc                                        <  4:498200/150‑1 (MQ=255)                                                                                                          cGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCTCCAGTTACTGGCGCAACt                                       <  2:574701/150‑1 (MQ=255)                                                                                                             gaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCATAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    <  3:491325/150‑1 (MQ=255)                                                                                                             gaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    >  2:228717/1‑150 (MQ=255)                                                                                                             gaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    <  2:961290/150‑1 (MQ=255)                                                                                                             gaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    >  4:580539/1‑150 (MQ=255)                                                                                                                gATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgc                                             >  1:487446/1‑138 (MQ=255)                                                                                                                gATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgc                                             <  2:487446/138‑1 (MQ=255)                                                                                                                  tGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCTTTTGATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCg                               <  1:460892/150‑1 (MQ=255)                                                                                                                  tGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCg                               <  2:323764/150‑1 (MQ=255)                                                                                                                  tGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCACATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCg                               <  1:461006/150‑1 (MQ=255)                                                                                                                   gTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCgg                              >  2:490652/1‑150 (MQ=255)                                                                                                                     ccGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCa                            >  3:280344/1‑150 (MQ=255)                                                                                                                             cTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              >  4:70733/1‑76 (MQ=255)                                                                                                                             cTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              <  3:70733/76‑1 (MQ=255)                                                                                                                                cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGc                                                                                                                            >  2:828558/1‑43 (MQ=255)                                                                                                                                cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGc                                                                                                                            <  1:828558/43‑1 (MQ=255)                                                                                                                                            aTTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCaaa                          >  2:398835/1‑129 (MQ=255)                                                                                                                                            aTTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCaaa                          <  1:398835/129‑1 (MQ=255)                                                                                                                                             ttCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCgcgc    <  4:433122/150‑1 (MQ=255)                                                                                                                                               cccGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTg  <  3:580539/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTG  >  NC_000913_3_pae_tpiA/4158558‑4158849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑