New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3380606	110 (1.380)	3 (0.040)	3/260	NT	2.7%	intergenic (+17/‑137)	yhcB/degQ	DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
	?	NC_000913_3_pae_tpiA	= 3380611	108 (1.430)					intergenic (+22/‑132)	yhcB/degQ	DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
Rejected: Frequency below/above cutoff threshold.

CTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3380452‑3380606 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcaATGGCTGCGCCCGAAAAATAAATTAGTCGCGCTTCGCGCCAGTACGCAGCAGGCCGGATGCGCCTTCAGAATAGTCGCGAGGCATCTGCACCGGTGCCTGATCGTTGCTGGCTTCAGACTCTGCCAGACGATTACGGAACGGGTTTG  <  NC_000913_3_pae_tpiA/3380611‑3380465                                                                                                                                                                                                                                                                                                                CTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGCAGCGCGACTAATTTATTTTTCG                                                                                                                                                           >  2:639181/1‑149   tccgtCTGAAGCAAACCCGTTCCGGAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGG                                                                                                                                                         <  2:1025021/144‑1       GCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCA                                                                                                                                                     <  1:1126735/149‑1                            AATCGTCTGGCCGAGTCTGAAGCCAGCAACGATCAGGCACCTGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCGGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAATGGCTGCGCCCGAAAAATAA                                                                                                                                <  2:474501/149‑1                                                                                     GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAATGGCTGCGCCCGAAAAATAAATTAGTCGCGCTTCGCGCCAGTA                                                                                                         >  1:981259/1‑115                                                                                     GACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAATGGCTGCGCCCGAAAAATAAATTAGTCGCGCTTCGCGCCAGTA                                                                                                         <  2:981259/115‑1                                                                                                                                                      GGCGCAATGGCTGCGCCCGAAAAATAAATTAGTCGCGCTTCGCGCCAGTACGCAGCAGGCCGGATGCGCCTTCAGAATAGTCGCGAGGCATCTGCACCGGTGCCTGATCGTTGCTGGCTTCAGACTCTGCCAGACGATTACGGAACGGG      >  1:743400/1‑149                                                                                                                                                       cctCAATGACTGCCCCCGAAAAATAAATTAGCCGCGCTTCGCGCCAGTACGCAGCAGGCCGGATGCGCCTTCATAACAGTCGCGAGCCATCTGCACCGGTGCCTGATCGTTGCTGGCTTCAGACTCTGCCAGACGATTACGGAACGGGT     <  2:59863/146‑1                                                                                                                                                          CAATGGCTGCGCCCGAAAAATAAATTAGTCGCGCTTCGCGCCAGTACGCAGCAGGCCGGATGCGCCTTCAGAATAGTCGCGAGGCATCTGCACCGGTGCCTGATCGTTGCTGGCTTCAGACTCTGCCAGACGATTACGGAACGGGTTTG  <  2:284059/149‑1                                                                                                                                                                                                                                                                                                                CTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3380452‑3380606 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcaATGGCTGCGCCCGAAAAATAAATTAGTCGCGCTTCGCGCCAGTACGCAGCAGGCCGGATGCGCCTTCAGAATAGTCGCGAGGCATCTGCACCGGTGCCTGATCGTTGCTGGCTTCAGACTCTGCCAGACGATTACGGAACGGGTTTG  <  NC_000913_3_pae_tpiA/3380611‑3380465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.