Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	77 [0]	[0] 77	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	71 (0.950)	54/258	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAAT  >  NC_000913_3_pae_tpiA/1978355‑1978649                                                                                                                                                     |                                                                                                                                                    aGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGt                                                                                                                                                    <  1:784500‑M1/149‑2 (MQ=255)    aaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtata                                                                                                                                                 <  2:915254‑M1/149‑5 (MQ=255)      tGGGTCTTTACACTTATCTAAGATTTTTCATAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataag                                                                                                                                               >  2:1190863‑M1/1‑143 (MQ=255)         gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                            <  1:1302529‑M1/149‑10 (MQ=255)            tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                         >  1:1209145‑M1/1‑137 (MQ=255)                     aTCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtttt                                                                                                                                <  2:1164960‑M1/149‑22 (MQ=255)                      tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACAGAAAACACTCCATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                               >  1:988730‑M1/1‑127 (MQ=255)                      tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                               >  1:291205‑M1/1‑127 (MQ=255)                      tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttg                                                                                                                               >  1:223258‑M1/1‑127 (MQ=255)                        tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                                                                             >  1:375743‑M1/1‑125 (MQ=255)                        tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                                                                             >  1:1292972‑M1/1‑125 (MQ=255)                         aaGATTTTTCAGAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                                                                            <  2:1156571‑M1/149‑26 (MQ=255)                          aGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                                                                           >  1:154932‑M1/1‑123 (MQ=255)                           gATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgt                                                                                                                          <  1:1300945‑M1/149‑28 (MQ=255)                            aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGCGGGGGGCTGCGCTTTTCAATAATGCGTGA‑TGCAGATCACACTAACCCCTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                                                                         >  1:1102305‑M1/1‑121 (MQ=255)                                   cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                  >  1:148919‑M1/1‑114 (MQ=255)                                   cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGAGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacatttgttttgggtgtatttaaat                                                                                                                  >  2:710917‑M1/1‑114 (MQ=255)                                   cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGAGCTGCGATTTTCAATAATGCGTAA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcaaacttttgtttttgttgtatttcaat                                                                                                                  >  2:157521‑M1/1‑114 (MQ=255)                                    tcaatcGACGACACTTTACTCGTCACTACACGCACATACAACGGAAGGGGGCTGCGATTTTCAATAATGCTTGA‑TGTAGATCACACAAAACACTCAATTACTTAACATAAATTtataagtcatacttttgtttttggtgtatttcaatc                                                                                                                 <  2:1140559‑M1/147‑37 (MQ=255)                                    tAAATCGACGCAACTGTACTCGTCACTACACGCACATGCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatncttttgttttgggtgtatttcaatc                                                                                                                 >  1:929038‑M1/1‑113 (MQ=255)                                     aaaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatct                                                                                                                <  1:148508‑M1/149‑38 (MQ=255)                                            cGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAACCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatccgttaaaa                                                                                                         >  1:1002159‑M1/1‑105 (MQ=255)                                             gaaactGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTATCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaac                                                                                                        <  1:781420‑M1/147‑46 (MQ=255)                                             gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttaggtgtatttcaatctgttaaaaa                                                                                                        <  1:1223850‑M1/149‑46 (MQ=255)                                                aCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtt                                                                                                     >  1:642253‑M1/1‑101 (MQ=255)                                                  tGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttt                                                                                                   >  1:64771‑M1/1‑99 (MQ=255)                                                   gTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                  <  1:1100194‑M1/149‑52 (MQ=255)                                                   gTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                  <  1:602221‑M1/149‑52 (MQ=255)                                                   gTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                  <  1:1374560‑M1/149‑52 (MQ=255)                                                   gTACTCGGCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                  <  1:1083815‑M1/149‑52 (MQ=255)                                                    tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgatttgcgtgtatttcaatctgttaaaaagtttttc                                                                                                 >  2:929736‑M1/1‑97 (MQ=255)                                                     aCTCGTCATTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATG‑GTCATTAGTGTGTTCACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                                <  1:332292‑M1/149‑54 (MQ=255)                                                     aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGGTTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTGACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                                <  1:203452‑M1/149‑54 (MQ=255)                                                          tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TACAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacg                                                                                           >  1:777343‑M1/1‑91 (MQ=255)                                                                cacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagca                                                                                     <  1:482297‑M1/149‑65 (MQ=255)                                                                 acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                                    >  1:952956‑M1/1‑84 (MQ=255)                                                                 acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                                                                    >  1:952910‑M1/1‑84 (MQ=255)                                                                     acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                                >  2:790064‑M1/1‑80 (MQ=255)                                                                       aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCCCCCAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                              <  1:1251585‑M1/149‑72 (MQ=255)                                                                       aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                              >  1:163989‑M1/1‑78 (MQ=255)                                                                       aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACGCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtattacaatatgttaaaaagtttttcgctacgctggcaagcaaaa                                                                              >  2:6721‑M1/1‑78 (MQ=255)                                                                       aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGGTA‑TGCAGACCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                              <  1:1251513‑M1/149‑72 (MQ=255)                                                                        tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctactcgatcaatcaaaaa                                                                             >  2:978999‑M1/1‑77 (MQ=255)                                                                        tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                             >  1:36636‑M1/1‑77 (MQ=255)                                                                           aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                          >  1:703703‑M1/1‑74 (MQ=255)                                                                           aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                          <  2:1215076‑M1/149‑76 (MQ=255)                                                                             cGGAGGGGGGTCGCTATTTTCAATAATGCGTGA‑TGCAAATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                        <  1:662121‑M1/149‑78 (MQ=255)                                                                              ggAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctactgtagcaagcaaaaatgaaac                                                                       >  2:642268‑M1/1‑71 (MQ=255)                                                                                aGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                     <  2:951179‑M1/149‑81 (MQ=255)                                                                                  gggggCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgatacgctatcaatcaaaaattaaacaaga                                                                   >  2:105707‑M1/1‑67 (MQ=255)                                                                                   ggggCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaa                                                                  <  1:587727‑M1/149‑84 (MQ=255)                                                                                          cGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcga                                                           >  1:1053384‑M1/1‑59 (MQ=255)                                                                                             ttttCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat                                                        >  1:434382‑M1/1‑56 (MQ=255)                                                                                                tCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggg                                                     <  2:198227‑M1/149‑97 (MQ=255)                                                                                                 cAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacacgaataatagaactggta                                                    >  2:1265598‑M1/1‑52 (MQ=255)                                                                                                  aataatGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaacatgaaacaggaataatcgaaatgggat                                                   >  1:713857‑M1/1‑51 (MQ=255)                                                                                                  aataatGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                                   >  1:713452‑M1/1‑51 (MQ=255)                                                                                                    taatGCGTGA‑TGCATCTCACACAAAACACTCAATTACCTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgt                                                 <  2:99314‑M1/149‑101 (MQ=255)                                                                                                        gCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                             >  2:1314892‑M1/1‑45 (MQ=255)                                                                                                            gA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                         <  2:1174904‑M1/149‑109 (MQ=255)                                                                                                             gaagaagatcaCACAAAACTCTCAATTACTTAAAATACATGtattagtcaaacttttgttttgggtgtatttcaatctgttaaaaagttattctctacaatagcaagcaaaaatgaaacatgaataatcgaaatgggatgttgcgcaca                                         <  2:1174996‑M1/144‑109 (MQ=255)                                                                                                                 cAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtca                                     >  1:1115122‑M1/1‑37 (MQ=255)                                                                                                                    aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgtacagtcaaaa                                  >  2:758666‑M1/1‑34 (MQ=255)                                                                                                                    aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                                  <  1:1085515‑M1/149‑116 (MQ=255)                                                                                                                       acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                               >  2:546730‑M1/1‑31 (MQ=255)                                                                                                                         acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataact                             >  2:974470‑M1/1‑29 (MQ=255)                                                                                                                           aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                           >  1:446785‑M1/1‑27 (MQ=255)                                                                                                                              acacTCAATTACATAACATAAATGtataagtcatacttttgttttgggtttaattcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccg                        <  2:503975‑M1/149‑126 (MQ=255)                                                                                                                                  tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa                    >  1:1260570‑M1/1‑20 (MQ=255)                                                                                                                                    aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaata                  >  2:1399540‑M1/1‑18 (MQ=255)                                                                                                                                      ttACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacaatcaaaataactcacgctaaatcat                >  2:114694‑M1/1‑16 (MQ=255)                                                                                                                                       tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc               <  1:790064‑M1/149‑135 (MQ=255)                                                                                                                                       tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc               <  2:223258‑M1/149‑135 (MQ=255)                                                                                                                                         cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtaaaaataactcaacatgaataatcat             >  2:440177‑M1/1‑13 (MQ=255)                                                                                                                                          ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc            >  1:1303280‑M1/1‑12 (MQ=255)                                                                                                                                                   aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa   <  2:1201881‑M1/149‑147 (MQ=255)                                                                                                                                                    tGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcacagaaaataataatgtgatataaat  >  2:981759‑M1/1‑2 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGA‑TGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAAT  >  NC_000913_3_pae_tpiA/1978355‑1978649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑