Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 1,722,472 G→A 7.3% V118I (GTC→ATC)  ydhK → putative efflux protein (PET) component of YdhJK efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,722,4720GA 7.3% 174.0 / 3.3 64V118I (GTC→ATC) ydhKputative efflux protein (PET) component of YdhJK efflux pump
Reads supporting (aligned to +/- strand):  ref base G (36/23);  new base A (3/2);  total (39/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

GGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTTGTGCGGCGGCATGATGAT  >  NC_000913_3_pae_tpiA/1722328‑1722620
                                                                                                                                                |                                                                                                                                                    
ggACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTcgc                                                                                                                                                  <  1:142125/149‑1 (MQ=255)
   cGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGCGCGCACTTCACGAATAACGTCGCGTa                                                                                                                                               <  1:818509/149‑1 (MQ=255)
    gCATCGGTGGCAGTGTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGTGCGACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCAGGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTAt                                                                                                                                              <  1:191528/149‑1 (MQ=255)
          cTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTCGGCACTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCAttt                                                                                                                                        >  1:1103208/1‑149 (MQ=255)
                  ttGCTCGGAGCCATTGCTGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTTTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGc                                                                                                                                <  1:1190519/149‑1 (MQ=255)
                      tCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGc                                                                                                                            >  1:202341/1‑149 (MQ=255)
                      tCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGc                                                                                                                            >  1:201991/1‑149 (MQ=255)
                      tCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTAGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGc                                                                                                                            <  2:1178467/149‑1 (MQ=255)
                          aGCCATTGCGGCATGTCTTCTTGCCGGGCATACGTTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTaca                                                                                                                        <  2:846944/149‑1 (MQ=255)
                             cATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACgg                                                                                                                     <  2:731130/149‑1 (MQ=255)
                               ttGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCt                                                                                                                   >  1:966556/1‑149 (MQ=255)
                                 gCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTCTGACTTTCAACTGGCGGGCTAACCGGATGc                                                                                                                 >  2:580859/1‑149 (MQ=255)
                                 gCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGc                                                                                                                 <  2:177916/149‑1 (MQ=255)
                                   ggCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCca                                                                                                               >  1:855361/1‑149 (MQ=255)
                                   ggCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTAACCGGCTGCca                                                                                                               >  2:513649/1‑149 (MQ=255)
                                        tGCTTCTTGCCGGACATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCatcatc                                                                                                          >  2:908322/1‑149 (MQ=255)
                                          cttcttGCCGGGCATACGCTCAATGAGCCGTTGTTTTTTTTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGc                                                                                                        <  1:908913/149‑1 (MQ=255)
                                              ttGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCttt                                                                                                    <  1:1211283/149‑1 (MQ=255)
                                                        tACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTa                                                                                          >  2:475652/1‑149 (MQ=255)
                                                        tACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGAAGGTTa                                                                                          >  2:475293/1‑149 (MQ=255)
                                                        tACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACCGCTGCCATCATCGCCTTTCCGCAGGTTa                                                                                          >  2:322853/1‑149 (MQ=255)
                                                         aCGCTCAAGGAGCCGTGGTTTTGTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTCTCCCTGGGCCCGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTaa                                                                                         <  1:2344/149‑1 (MQ=255)
                                                           gCTCAATGAGCCGTGGAGTTGTCTAGTTAGCATGGCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAata                                                                                       >  2:1409460/1‑149 (MQ=255)
                                                             tCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTGATAtt                                                                                     >  2:684878/1‑149 (MQ=255)
                                                               aaTGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTT‑ATATTACt                                                                                  >  2:1151453/1‑149 (MQ=255)
                                                                 tGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACTTGCGACAATGTCCACGTCTCGAATGCCGGTGCGTATGCATTTCAACACGTGGGCTATACGTCTTCCATCATCTCCTTTCCGAGGGGTAATATTACTg                                                                                 >  1:700762/1‑149 (MQ=255)
                                                                  gAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGa                                                                                <  2:870700/149‑1 (MQ=255)
                                                                  gAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGa                                                                                <  1:146811/149‑1 (MQ=255)
                                                                      cGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGgcc                                                                            <  1:1122087/149‑1 (MQ=255)
                                                                        tGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTTCACGGCTGCCATTATCACCTTTCCTATGGTTAATTTTAATAAGgcagg                                                                          >  1:352809/1‑146 (MQ=255)
                                                                        tGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGgccag                                                                          >  1:1173322/1‑149 (MQ=255)
                                                                          gTTTTGTCTAATGAGCATGTCGGCGTGGCAAGGCTTTTGTACCTGGGCCTGTGGGCACTTCACGAATAGCGTCGCGTATGCATTTCAACTGGCGGGGTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGgcctgcc                                                                        >  2:815616/1‑149 (MQ=255)
                                                                                             tCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCa                                                     >  2:649352/1‑149 (MQ=255)
                                                                                             tCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCa                                                     >  2:430763/1‑149 (MQ=255)
                                                                                                gCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGgc                                                  >  2:1369316/1‑149 (MQ=255)
                                                                                                  ttggcttggctTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGgcgc                                                <  2:700762/148‑1 (MQ=255)
                                                                                                  ctggcttgggtTGCATACCTGGGCCTGTTCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGgcgc                                                <  2:585420/148‑1 (MQ=255)
                                                                                                     gcttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACATCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGtt                                                                                           >  1:1356464/1‑103 (MQ=255)
                                                                                                     gcttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACATCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGtt                                                                                           >  1:1356703/1‑103 (MQ=255)
                                                                                                     gcttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACATCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGtt                                                                                           <  2:1356464/103‑1 (MQ=255)
                                                                                                     gcttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACATCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGtt                                                                                           <  2:1356703/103‑1 (MQ=255)
                                                                                                       ttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATCACATCGCCGTGATAGTACACCTCGCCGAGGTCACGGCTGCCATCATCGCCTTTCCGATGGATAATATTACTGAGGCCAGCCAGCGGTGGGATATCGCTCAGGCGCGCGtt                                           >  1:1039822/1‑149 (MQ=255)
                                                                                                       ttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGtt                                           >  1:551483/1‑149 (MQ=255)
                                                                                                       ttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTAAGGCGCGCGtt                                           >  2:128806/1‑149 (MQ=255)
                                                                                                       ttggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGCATAAGAGGACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGtt                                           >  1:1027690/1‑149 (MQ=255)
                                                                                                        tggcttTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGttt                                          >  2:1330017/1‑149 (MQ=255)
                                                                                                          gcttTTGTACCTGGGCCTGTGCGCACTTCACGAATATCGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGc                                        <  2:602256/149‑1 (MQ=255)
                                                                                                            ttttGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGGCAGCTGTGGGAAACCGGTCAGGCGGGCGTCTGCGa                                      >  2:476395/1‑149 (MQ=255)
                                                                                                            ttttGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGa                                      <  2:855361/149‑1 (MQ=255)
                                                                                                                    cTGGGCCTGTGAGCACTTCACAAATAACGTCGCGTATGCATTTCAACTGTCGTGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGGGGTAATAg                              >  2:1408646/1‑149 (MQ=255)
                                                                                                                     tGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGt                             >  2:487448/1‑149 (MQ=255)
                                                                                                                      gggCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTc                            >  2:1167323/1‑149 (MQ=255)
                                                                                                                           tgtgCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAt                       >  2:795582/1‑149 (MQ=255)
                                                                                                                           tgtgCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAt                       >  1:214508/1‑149 (MQ=255)
                                                                                                                           tatgCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAt                       >  1:421992/3‑149 (MQ=255)
                                                                                                                             tgCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAttt                     >  1:466820/1‑149 (MQ=255)
                                                                                                                             tgCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCAAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGCGGTAATAGTCGGTAttt                     >  2:986/1‑149 (MQ=255)
                                                                                                                              gcgcACTTCACGAATAATGTCGCCTATGCATTTCAACTGGCCGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAtttt                    <  2:85392/149‑1 (MQ=255)
                                                                                                                              gcgcACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAtttt                    <  1:1311626/149‑1 (MQ=255)
                                                                                                                              gcgcACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTAtttt                    <  1:1311575/149‑1 (MQ=255)
                                                                                                                               cgcACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTtg                   >  1:419778/1‑149 (MQ=255)
                                                                                                                                gcACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTtgt                  <  1:329241/149‑1 (MQ=255)
                                                                                                                                     tCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTTGTgcggc             <  1:1143587/149‑1 (MQ=255)
                                                                                                                                       aCGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTTGTGcggcgg           >  2:1132548/1‑149 (MQ=255)
                                                                                                                                                gTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCCTCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTTGTGCGGCGGCatgatgat  <  1:912097/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGACGCATCGCTGGCAGTTTGCTCGGAGCCATTGCGGCACTGCTTCTTGCCGGGCATACGCTCAATGAGCCGTGGTTTTTTCTATTGAGCATGTCGGCGTGGCTTGGCTTTTGTACCTGGGCCTGTGCGCACTTCACGAATAACGTCGCGTATGCATTTCAACTGGCGGGCTACACGGCTGCCATCATCGCCTTTCCGATGGTTAATATTACTGAGGCCAGCCAGCTGTGGGATATCGCTCAGGCGCGCGTTTGCGAGGTAATAGTCGGTATTTTGTGCGGCGGCATGATGAT  >  NC_000913_3_pae_tpiA/1722328‑1722620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑