Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 2,091,738 C→T 7.2% V214V (GTC→GTT)  hisD → bifunctional histidinal dehydrogenase/ histidinol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,091,7380CT 7.2% 182.1 / 2.6 64V214V (GTC→GTT) hisDbifunctional histidinal dehydrogenase/ histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base T (2/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

TTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCC  >  NC_000913_3_pae_tpiA/2091590‑2091886
                                                                                                                                                    |                                                                                                                                                    
ttGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTc                                                                                                                                                      <  1:121517/149‑1 (MQ=255)
     gATGAGATCCTTTATGCGGCGCAGCTGTGTGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGTTAACGCCTTTGTCACCgc                                                                                                                                                 >  2:272901/1‑148 (MQ=255)
      aTGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCTGGGTAACGCCTTTGTCACCGaa                                                                                                                                                <  1:743705/149‑1 (MQ=255)
          gaTCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAACATCTTCGGGCCGGGTAACGCCTTTGTCCCCGAAGCGa                                                                                                                                            >  2:253266/1‑149 (MQ=255)
          gaTCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGa                                                                                                                                            >  2:812862/1‑149 (MQ=255)
                 tacgcggcgCATCGCGGCGGTGTGCAGGACGTGTTTAACGCCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACCGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCa                                                                                                                                     <  1:1083041/146‑1 (MQ=255)
                         gcAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTTACCGAAGCGAAACGTCAGGTGAGcc                                                                                                                             >  1:599075/1‑149 (MQ=255)
                         gcAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTTACCGAAGCGAAACGTCAGGTGAGcc                                                                                                                             >  1:599242/1‑149 (MQ=255)
                                tgCGGTGTGCAGGACGTGGTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCt                                                                                                                      <  1:1263195/149‑1 (MQ=255)
                                 gCGGTGTGCAGGACGTGTTTCACGTCGGCGGCGCACAAGAAGGTGGTGCACTGGCGTGTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTg                                                                                                                     <  2:1211312/149‑1 (MQ=255)
                                  cGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTgg                                                                                                                    >  2:913874/1‑149 (MQ=255)
                                      gtgCAGGACGTGTTTAACGGGGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTGCGGGCCGTGTAACGCCTTTGTCACCGAAGCGAAACGTCGGGTGAGCCAGCGTCTGGACgg                                                                                                                <  1:1018539/149‑1 (MQ=255)
                                       tgCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGt                                                                                                               >  1:1223728/1‑149 (MQ=255)
                                           ggACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTTACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTgcgg                                                                                                           <  2:599075/149‑1 (MQ=255)
                                           ggACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTTACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTgcgg                                                                                                           <  2:599242/149‑1 (MQ=255)
                                            gACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTgcggc                                                                                                          <  1:1291005/149‑1 (MQ=255)
                                               gtgtTTAACGGCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgat                                                                                                       <  2:1078336/149‑1 (MQ=255)
                                               gcgtTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgat                                                                                                       <  1:873825/147‑1 (MQ=255)
                                                tgtTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGTTGCGTcgatc                                                                                                      >  1:183105/1‑149 (MQ=255)
                                                tgtTTAACGTCGGCGGCGCACAGACCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgatc                                                                                                      <  1:1393555/149‑1 (MQ=255)
                                                  tttAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGcgatcga                                                                                                    >  1:673748/1‑149 (MQ=255)
                                                  tttAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGAACTGCATGCTGGAATTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCCCCGCAGCGAAACGTCAGGGGAGCCAGCGTCTGGACGGTGCGGcgaacga                                                                                                    >  1:977533/1‑149 (MQ=255)
                                                    tAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTTACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGata                                                                                                  <  1:862922/149‑1 (MQ=255)
                                                     aaCGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGatat                                                                                                 >  1:1083394/1‑149 (MQ=255)
                                                        gTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCACAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTAGGGCCGGTACGGCGATCGATCTGcc                                                                                              >  2:583033/1‑149 (MQ=255)
                                                                 gcACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGccc                                                                                     >  1:1022903/1‑149 (MQ=255)
                                                                    caGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAGTCTTCGGGCCGGGTAACGCCTTGGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCggc                                                                                  >  1:1092961/1‑147 (MQ=255)
                                                                    caGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCAGGGTAACGGGTTTGTCACCGGAGCGAAACGTCAGGTGAGGCAGCGTCTGGACGGTGCGGCGGTCGTTTTGCCCGCTGGCCGGtg                                                                                  >  2:157904/1‑148 (MQ=255)
                                                                      ggCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCgg                                                                                <  2:1093055/149‑1 (MQ=255)
                                                                       gCCATTGCCGCCCTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATAGGCCCGCACGCCCCGCGGa                                                                               >  2:171554/1‑149 (MQ=255)
                                                                        ccATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGaa                                                                              <  1:245095/149‑1 (MQ=255)
                                                                        ccATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGaa                                                                              <  1:287330/149‑1 (MQ=255)
                                                                        ccATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGaa                                                                              <  1:1302710/149‑1 (MQ=255)
                                                                        ccATTGCCCCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGaa                                                                              <  1:387891/149‑1 (MQ=255)
                                                                        ccATGGCCGCACTGGCGTTTGGTACGGAATCGGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGaa                                                                              <  2:454795/149‑1 (MQ=255)
                                                                          aTTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAACATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGt                                                                            >  2:1102724/1‑149 (MQ=255)
                                                                           ttGCCGCACTGGCGTTTGGTACGGCATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTg                                                                           >  1:1048569/1‑149 (MQ=255)
                                                                              gcGCACGGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTg                                                                        <  1:1385183/148‑1 (MQ=255)
                                                                               cGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTgg                                                                       >  2:954458/1‑149 (MQ=255)
                                                                               cGCACTGGCGTTTGGTACCGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTgg                                                                       <  1:646233/149‑1 (MQ=255)
                                                                                gCACTGGCGTTTGGTACGGTATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGTCGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAATGCTGGt                                                                      <  1:1020036/149‑1 (MQ=255)
                                                                                             gTACGGAATCTGTGCCAAAAGTGGCCACCGTCAACGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGACCGTCGGGAGTGCTGGTGATTTCTGACaac                                                         >  2:311206/1‑147 (MQ=255)
                                                                                             gTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGGAGTGCTGGTGATTGCTGACAgc                                                         >  1:374529/1‑149 (MQ=255)
                                                                                              tACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAgcg                                                        >  1:205023/1‑149 (MQ=255)
                                                                                                    aTCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTa                                                  <  2:1330798/149‑1 (MQ=255)
                                                                                                    aTCGGTGCCAAAAGCGGACAAAAGCGTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTa                                                  <  2:741982/149‑1 (MQ=255)
                                                                                                     tCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTAc                                                 >  1:921515/1‑149 (MQ=255)
                                                                                                        gtgCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCGGGTGGTTGCTGACAGCGGCGCTACGcc                                              >  1:826925/1‑149 (MQ=255)
                                                                                                          gCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCgg                                            >  1:858684/1‑149 (MQ=255)
                                                                                                           ccAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGa                                           <  1:1143960/149‑1 (MQ=255)
                                                                                                            cAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGAt                                          >  1:74361/1‑149 (MQ=255)
                                                                                                             aaaaGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGAtt                                         <  2:580971/149‑1 (MQ=255)
                                                                                                                 gTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGt                                     <  1:67644/149‑1 (MQ=255)
                                                                                                                  tGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTg                                    <  1:353565/149‑1 (MQ=255)
                                                                                                                       aaaaTCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGTTGTGCTGGTGATTGCTGACAGCGGAGCTACGCCGGATTTCGTGGCTTc                               >  1:330785/1‑149 (MQ=255)
                                                                                                                       aaaaTCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGAGTTCGTGGCTTc                               >  2:1368328/1‑149 (MQ=255)
                                                                                                                         aaTCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTg                             >  1:630777/1‑149 (MQ=255)
                                                                                                                            tgtctggCCGGGAAACGTCTTTGTCACCGTAGCTAAACGTCAGGTGAGCCAGCGTCTTGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGAtt                          <  2:31835/144‑1 (MQ=255)
                                                                                                                             ttCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGAttt                         >  1:1261071/1‑149 (MQ=255)
                                                                                                                              tCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTg                        <  2:967031/149‑1 (MQ=255)
                                                                                                                                    cGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGctctct                  <  2:205023/149‑1 (MQ=255)
                                                                                                                                            gCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCCGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGa          <  2:601506/149‑1 (MQ=255)
                                                                                                                                             ccTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGaa         <  2:1146094/149‑1 (MQ=255)
                                                                                                                                             ccTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGCCTGaa         <  2:1048569/149‑1 (MQ=255)
                                                                                                                                                ttGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAacac      <  1:1188232/149‑1 (MQ=255)
                                                                                                                                                    cACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGcc  <  1:907615/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGGGTAACGCCTTTGTCACCGAAGCGAAACGTCAGGTGAGCCAGCGTCTGGACGGTGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCC  >  NC_000913_3_pae_tpiA/2091590‑2091886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑