Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 2,106,741 A→T 100% S162S (TCT→TCA)  wbbH ← O‑antigen polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,106,7410AT100.0% 311.4 / NA 92S162S (TCT→TCA) wbbHO‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (46/46);  total (46/46)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_pae_tpiA/2106596‑2106886
                                                                                                                                                 |                                                                                                                                                  
ctctATTAACACCTACGATGAATGCATAAGAGATGATAACAATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑Ac                                                                                                                                                >  2:1224271/1‑149 (MQ=255)
ctctATTAACACCTACGATGAAAGTATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑Ac                                                                                                                                                >  1:739537/1‑149 (MQ=255)
 tctATTAACACCTACGATGAATGCATAAGAGATGATAAGCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑Acc                                                                                                                                               >  2:1017269/1‑149 (MQ=255)
 ggtATTAACACCTGCGATGAATGCATAAGCGATGATACCCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACACGTAAAGTAAATGTTTTACTTACNTTTGTATTAGTAAATTTTTTTG‑Acc                                                                                                                                               >  1:931415/3‑149 (MQ=255)
   tATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ATCaa                                                                                                                                             >  2:1388555/1‑149 (MQ=255)
        tcacaTACGAGGAATGCATAAGAGATGATAACCATAAAGTCAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAATACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCaaataaa                                                                                                                                        <  2:326009/148‑1 (MQ=255)
        acacCTACGATGAATGCAGAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCATTATATTAGTAAATTTTTTTG‑ACCaaataaa                                                                                                                                        <  2:526713/149‑1 (MQ=255)
          acCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAaataaata                                                                                                                                      >  1:865330/1‑149 (MQ=255)
           ccTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAataaataa                                                                                                                                     <  1:38443/149‑1 (MQ=255)
            cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACTAATtaaataaa                                                                                                                                    >  2:1109230/1‑149 (MQ=255)
            cTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAAtaaataaa                                                                                                                                    >  1:1414434/1‑149 (MQ=255)
             tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAg                                                                                                                                   >  2:255160/1‑149 (MQ=255)
             tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAg                                                                                                                                   >  2:1212616/1‑149 (MQ=255)
             tACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAg                                                                                                                                   <  2:903963/149‑1 (MQ=255)
                         aaaaGAAATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       <  2:983006/147‑1 (MQ=255)
                         aTAAGAGATGTTAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTATATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATCAATAAAGCAAAAGTAGTTa                                                                                                                       <  2:1089229/149‑1 (MQ=255)
                         aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTGAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       >  2:909073/1‑149 (MQ=255)
                         aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       <  2:1089334/149‑1 (MQ=255)
                         aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       <  2:807551/149‑1 (MQ=255)
                         aTAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTAATTACCTCTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       >  1:970140/1‑149 (MQ=255)
                         aTAAGAGACGATAACCATAAAGACAATTTCCTTATCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAGAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTa                                                                                                                       <  1:662429/149‑1 (MQ=255)
                            aggaatgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGaa                                                                                                                    <  2:91036/145‑1 (MQ=255)
                                atgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATAAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGAt                                                                                                                >  2:1394348/1‑149 (MQ=255)
                                 tgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGAtt                                                                                                               <  2:1145772/149‑1 (MQ=255)
                                  gatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTg                                                                                                              <  1:1002945/149‑1 (MQ=255)
                                  gatAACCATAAAGACAATTTGCTTAACAGTGTTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAGTAAATAAAGCAAAAGTAGTTAGAATGATTg                                                                                                              >  1:1048468/1‑149 (MQ=255)
                                     aaacATAAAGACAATTTTCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCt                                                                                                           <  1:497091/146‑1 (MQ=255)
                                       ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGAAGTTAGAATGATTGGCTga                                                                                                         >  2:553701/1‑148 (MQ=255)
                                         aTAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAATTAGTTAGAATGATTGGCTGCAt                                                                                                       <  1:723737/149‑1 (MQ=255)
                                         aTAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGGGGTGGTGTGTG‑ACCAAATAAATAAAGCAATAGTAGTTAGAATGATGGGCTGcgt                                                                                                       >  1:402065/1‑147 (MQ=255)
                                         aTAAAGACAATTTGATTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTACACGTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAt                                                                                                       <  1:1335071/149‑1 (MQ=255)
                                         aTAAAGACAAATTGCCTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTCCAATTGTATTAGTAAATTTTTTTG‑ACCAAAAAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAt                                                                                                       <  1:1335248/149‑1 (MQ=255)
                                         aTAAAGAAAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAt                                                                                                       <  1:356300/149‑1 (MQ=255)
                                          tAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCGAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATg                                                                                                      >  1:266227/1‑149 (MQ=255)
                                              gACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATg                                                                                                  >  2:1147440/1‑149 (MQ=255)
                                              gACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAACAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGAATGTATg                                                                                                  >  2:484358/1‑149 (MQ=255)
                                                 aaTTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGCATCGCTGCATGTATGCTg                                                                                               >  2:1269219/1‑149 (MQ=255)
                                                        ttcccAGTATTCAGTATAATTGCACAGATGCATCCAATAAAAACAAGTAAAGTAAATGGTTTACTTACCCTTGTATTAGTAAATTTTTTTG‑ACCACATAAATCAAGCACAAGTAGTTAGAATGATTGGCTGCATGTATGCTAAGAAAtc                                                                                        >  2:1145232/4‑148 (MQ=255)
                                                              gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGa                                                                                  <  1:66004/149‑1 (MQ=255)
                                                               tgttCAGTATAATTGCAAAAATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:854853/147‑1 (MQ=255)
                                                               tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 >  1:1335987/1‑149 (MQ=255)
                                                               tATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 >  1:1223259/1‑149 (MQ=255)
                                                               gaatCATTAGAACTGAAAAGATTAATACAAAAAAAAACATAAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt                                                                                 <  2:832142/146‑1 (MQ=255)
                                                                aTTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtg                                                                                <  2:598517/149‑1 (MQ=255)
                                                                 ttCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGAATTAAATCAA‑AAATCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                               >  1:1032829/1‑149 (MQ=255)
                                                                 ttCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAACTCAATAAACCAAAAGTAGTTATAATGATTGGCTACATTTATGCTGAGAAATTCCTTGAtgt                                                                               >  2:592956/1‑149 (MQ=255)
                                                                 ttCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                               >  2:199884/1‑149 (MQ=255)
                                                                 ttCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                               >  1:263992/1‑149 (MQ=255)
                                                                      tataATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCtt                                                                          <  1:174524/149‑1 (MQ=255)
                                                                        taATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                        <  2:1414434/149‑1 (MQ=255)
                                                                        taATTCCAAATATTAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATTATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                        <  2:324956/149‑1 (MQ=255)
                                                                          aTTGCAAAGATTAATAGAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTCTTTTG‑ACCAAATAACTAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAAc                                                                      <  1:464289/149‑1 (MQ=255)
                                                                              cAAAGATGAATACAATAAAAACAAGTAAACTAAATGTTTACCTTACCGTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGTTGTGTCTTCAACATCa                                                                  <  2:931415/149‑1 (MQ=255)
                                                                               aaaGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAg                                                                 >  1:1173499/1‑149 (MQ=255)
                                                                                aaGATCAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGc                                                                <  1:495028/149‑1 (MQ=255)
                                                                                  gATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAAAATCTGCAt                                                              >  2:91706/1‑149 (MQ=255)
                                                                                  gATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATCGTGGGCATGAAGGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAt                                                              >  1:472037/1‑149 (MQ=255)
                                                                                   aTGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtc                                                             >  2:63493/1‑149 (MQ=255)
                                                                                     gAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                           >  2:1075169/1‑149 (MQ=255)
                                                                                     gAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGCTTCCAGGTGTGCGGAGAAATTTCTTGATGTGTCTTCAACATCAGCGtctc                                                           >  1:108845/1‑149 (MQ=255)
                                                                                      aaTACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATctct                                                          <  2:865330/149‑1 (MQ=255)
                                                                                       aTACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCtt                                                         >  2:1112973/1‑149 (MQ=255)
                                                                                        tACAATAAAAACAAGTAAAGTAGATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTa                                                        <  2:1047103/149‑1 (MQ=255)
                                                                                          cAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATc                                                      >  1:1133259/1‑149 (MQ=255)
                                                                                           aaTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTT‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGAATCTCTTATCa                                                     >  2:754759/1‑149 (MQ=255)
                                                                                               aaaaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAAtt                                                 <  1:1125105/149‑1 (MQ=255)
                                                                                                      gtaaagtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAg                                          <  1:909073/149‑1 (MQ=255)
                                                                                                      gtaaagtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAg                                          >  1:86332/1‑149 (MQ=255)
                                                                                                      gtaaagtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAg                                          >  1:691907/1‑149 (MQ=255)
                                                                                                          agtacaTGTTTGACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                                      <  2:861684/149‑1 (MQ=255)
                                                                                                          agtaaaTCTTTTACTGACCATTGTATTAGTCACTTTTTTTG‑ACCAGAGAAAGAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTACGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                                      <  1:554473/149‑1 (MQ=255)
                                                                                                           gtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaag                                     >  1:584352/1‑149 (MQ=255)
                                                                                                               aTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGTGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCcaagtaag                                 <  1:1017269/149‑1 (MQ=255)
                                                                                                               aTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGATTTTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagtaag                                 <  1:1339841/149‑1 (MQ=255)
                                                                                                                tGTTTTACTTACCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGc                                >  2:562679/1‑149 (MQ=255)
                                                                                                                      acttacCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTcc                          <  1:1003211/149‑1 (MQ=255)
                                                                                                                        ttacCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTa                              <  2:21662/143‑1 (MQ=255)
                                                                                                                        ttacCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTa                              >  1:21662/1‑143 (MQ=255)
                                                                                                                         tacCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAAGAAATCAAAAGTAGTTATAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGa                       <  2:725771/149‑1 (MQ=255)
                                                                                                                          acCTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATCTAGCTAAGTAAGCTAGTCCCGaa                      <  1:1224271/149‑1 (MQ=255)
                                                                                                                            cTTTGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACt                    >  1:1029843/1‑149 (MQ=255)
                                                                                                                             tttGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                   >  1:215064/1‑149 (MQ=255)
                                                                                                                              ttGTATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgg                  <  2:643869/149‑1 (MQ=255)
                                                                                                                                 tATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAATCTAGTCCCGAACTGGTAg               >  1:1169434/1‑149 (MQ=255)
                                                                                                                                 tATTAGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAg               >  2:916357/1‑149 (MQ=255)
                                                                                                                                   ttAGTAAATTTTTTTG‑ACCACATAAATAAAGCAAAAGTAGTTAGAATGATTGGTCCTGCGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAATTGGTAGtt             <  1:1031859/149‑1 (MQ=255)
                                                                                                                                    tAGTAAATTTTTCTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTa            <  1:550492/149‑1 (MQ=255)
                                                                                                                                     aGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGGATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCATCATTTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTAc           <  2:440925/149‑1 (MQ=255)
                                                                                                                                     aGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTGACAGGTGGAAGTAAGCTAGTCCCGAACTGGTAGTTAc           <  1:1409853/149‑1 (MQ=255)
                                                                                                                                     aGTAAATTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTAc           <  1:445266/149‑1 (MQ=255)
                                                                                                                                         aaTTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGCACTGGTAGTTACtgac       >  1:608704/1‑146 (MQ=255)
                                                                                                                                         aaTTTTTTTG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTaa       <  1:199884/149‑1 (MQ=255)
                                                                                                                                              ttttG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCa  <  1:249140/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAG‑ACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTCA  >  NC_000913_3_pae_tpiA/2106596‑2106886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑