Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 3,563,755 T→C 6.7% intergenic (+237/+32) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,563,7550TC 6.7% 299.4 / 6.1 110intergenic (+237/+32)glpD/yzgLsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
Reads supporting (aligned to +/- strand):  ref base T (49/53);  new base C (4/4);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 113 total aligned reads displayed.

CTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCAATAATACCGGCAAG  >  NC_000913_3_pae_tpiA/3563607‑3563900
                                                                                                                                                    |                                                                                                                                                 
cTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAGGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCcatcat                                                                                                                                                   >  1:604088/1‑149 (MQ=255)
 tACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATa                                                                                                                                                  >  1:1248025/1‑149 (MQ=255)
   cggggTTTATGCAATATATGGAATTTGCAGGGTCTTGGAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTGTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATAcc                                                                                                                                                <  2:194771/146‑1 (MQ=38)
    gTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATAccc                                                                                                                                               <  2:466911/149‑1 (MQ=255)
       gTTTATGCAATATATTGAATTTGCAAAGTCTTGTAGGCCAGATAAGACGTTCACGTCTCATCCGGCATGAACAAAGCGCACTTTGTGGAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAATACCCCGCCATCATACCCacc                                                                                                                                            >  2:837308/1‑147 (MQ=25)
        tttATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAGAATCTAACTTACTTTTTAATTCAAGGAATTACCGCAAAGCCCACGCCATCATACACAACg                                                                                                                                           >  2:442798/1‑149 (MQ=255)
          tATGCAATATATTGAATTTTCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGGCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTa                                                                                                                                         <  2:293086/149‑1 (MQ=255)
           aTGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTaa                                                                                                                                        <  1:1280732/149‑1 (MQ=255)
            tGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAAGCTAACCTACTTTTTAATTCAGGGAATTACCGCAAACCCAACGACATCATACCCAACATaaa                                                                                                                                       >  2:223928/1‑148 (MQ=37)
              cAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACaa                                                                                                                                     >  1:1406828/1‑149 (MQ=255)
                atatatTGAATTTGCCTGGTCTTGTAAACCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCACCCCCAACGTAACaaaa                                                                                                                                   >  2:576525/1‑149 (MQ=38)
                atatatTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCACACCCAACGTAACaaaa                                                                                                                                   >  2:576580/1‑149 (MQ=255)
                atatatTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCACACCCAACGTAACaaaa                                                                                                                                   >  2:103812/1‑149 (MQ=255)
                atatatTGAATTTGCATGGTATTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAAGCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACaaaa                                                                                                                                   <  1:174994/149‑1 (MQ=255)
                    atTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACGTTTGAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCa                                                                                                                               <  1:916698/149‑1 (MQ=255)
                     ttGAATTTGCATGGTCTTGTAGGCCAGATAAGACGGTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAg                                                                                                                              <  1:754731/149‑1 (MQ=255)
                     tggAATTTGCATGGTCTTGTATGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAg                                                                                                                              <  2:688851/147‑1 (MQ=255)
                      tGATTTTGCATGGTCTTGTGGGCCAGATAAGAGGTTCACGTCGCAGCAGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAgg                                                                                                                             <  2:287616/149‑1 (MQ=39)
                       gAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGc                                                                                                                            >  1:1316618/1‑149 (MQ=255)
                         aTTTGCATGGTATTGTAGGCCAGATAAGACGTTCACGTCGCATGCGGCATGAACACAGCGCACTTTGTCAAAAATCTAACCGACTTTTGAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAGCTCAGGCAc                                                                                                                          >  1:814281/1‑149 (MQ=255)
                          ttggCATGGTCTTGTAGGCCAGATAAGACGTTAACGTCGCATCCGGCATGAACAAAGCGCACTTTGTAAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGGAAAGCCCACCACACGATATCAAACGTAACAAAACTCAGGccga                                                                                                                         >  2:813958/4‑146 (MQ=37)
                              cATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCACCATACCCAACGTACCAAAACCCAGGCAGGTTcc                                                                                                                     >  1:1159207/1‑149 (MQ=255)
                               aTGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTccc                                                                                                                    <  1:166584/149‑1 (MQ=255)
                               aTGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTccc                                                                                                                    >  1:1282390/1‑149 (MQ=255)
                                 ggTCTTGTAGGGTAGATAGGGCGCTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCa                                                                                                                  <  1:794435/149‑1 (MQ=39)
                                 ggTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCa                                                                                                                  <  2:1067180/149‑1 (MQ=255)
                                              aGATAAGACGTACACGTCGCATCCGGCATGAACAAAGAGCACTTTGTCAAAAATCTAACATACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGAACGCTCCCCACCCGCCCCggcag                                                                                                     >  2:779514/1‑145 (MQ=255)
                                                aTAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGaaaa                                                                                                   <  2:1275145/149‑1 (MQ=255)
                                                  aaGACGTTCACGTCGCATCCGTCATGAAAAAAGCCCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAAt                                                                                                 <  1:1235469/149‑1 (MQ=255)
                                                  aaGACCGTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAAt                                                                                                 <  1:1066297/149‑1 (MQ=255)
                                                   aGACGTTCACGTCGCATCCGGCATGAACAATGCGCACTTTGTCAAAAATCCAACCTACTTTTTAATTCAGGGAATTACCGCAAAACCGACGCTAGCATACCCGCATTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATa                                                                                                <  1:1040828/149‑1 (MQ=255)
                                                   aGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATa                                                                                                <  1:789054/149‑1 (MQ=255)
                                                   aGACGTTCACGTCGCACCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAACTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATa                                                                                                <  2:1260375/149‑1 (MQ=255)
                                                   aGACGGTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCACGCCCCCTTGGGACTTGTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATa                                                                                                <  1:41162/149‑1 (MQ=255)
                                                    gACGTTCACGTCGCATCTGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAg                                                                                               <  2:650807/149‑1 (MQ=255)
                                                    gACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAACTCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCCTCATACCCAACGTAACAAAACCCACACCCATACCCAACTCACCCCGAGACAAAAAg                                                                                               >  2:414707/1‑149 (MQ=255)
                                                     aCGTTCACGTCGCATCCGGCATGAACAAAGTGCACTTTGTCAAAAATCTAACCTACTTTTTAGTTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAGAATCAGGCACGTTACCCACTCGCCCCGAGAAAAATAGc                                                                                              >  2:1268250/1‑149 (MQ=255)
                                                     aCGTTCACGTCGCATCCGGCAAGAACACAGCGCACTTTGCCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGc                                                                                              <  1:1012217/149‑1 (MQ=255)
                                                      cGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCa                                                                                             <  1:645270/149‑1 (MQ=255)
                                                          cACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGCAAAATAGCATTaa                                                                                         <  2:171639/149‑1 (MQ=255)
                                                              tCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATgcg                                                                                     >  1:1350492/1‑149 (MQ=255)
                                                                gCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAGATTGGCCTTAATGCGcc                                                                                   >  1:61864/1‑149 (MQ=255)
                                                                 cATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACAGCAAAGCCAACGCCATAATACCCAACGTAACAACACTCAGGCACGTTCACCACTCGCCACGAGAAAAATCGCATTCATACGcca                                                                                  >  2:23511/1‑148 (MQ=255)
                                                                  aTCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCa                                                                                 >  2:951683/1‑149 (MQ=255)
                                                                  aTCCGGCATGAACAAAGCGAACAAAGTCAAAAATCTCCCGCACCTTATCTTGCATTTAATTACCGCAACGACCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCa                                                                                 <  1:1026282/149‑1 (MQ=255)
                                                                   tCCGGCATGAACAAAGCGCACTTTGTCAACAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAg                                                                                >  1:945160/1‑149 (MQ=255)
                                                                       gCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGAGCCCAGCGcc                                                                            >  1:43032/1‑149 (MQ=255)
                                                                        cATGAACAAGGCGCACTGTGTCAAAAATCCAACCTACTTTTTAATTCAGGGAATGACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCa                                                                           <  1:173807/149‑1 (MQ=255)
                                                                         aTGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATAACCGCAAATCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACCCGCCCCGAGAAAAATAGCATTAATGAGCCCAGCGccgg                                                                          >  1:702304/1‑147 (MQ=255)
                                                                          taaataaaGCCCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCACAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCTCACCAGCCCCGAGACGAAAAACAGTCATACGCCGAGCGCCAGc                                                                         >  2:608058/6‑149 (MQ=255)
                                                                          tGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGc                                                                         >  1:887652/1‑149 (MQ=255)
                                                                           gAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTATTTTATTGTATTACCGAAAAGCCCACGCCATCATACCCAACGTAACAAAACTTAGGCACGTTCCCCACTCGCCCCGAGAAAAATAACATTAATGCGCCAAGAGCCAGCa                                                                        >  2:853265/1‑149 (MQ=255)
                                                                           gAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGccacca                                                                        >  2:455148/1‑149 (MQ=255)
                                                                           gAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCa                                                                        <  2:421711/149‑1 (MQ=255)
                                                                             aCAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATa                                                                      >  1:63787/1‑149 (MQ=255)
                                                                             aCAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGAAAAGCCCACGCCATCATACCCAACAAAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAAACAAATAACATTAACGCGTCCAGAGCCATCATa                                                                      >  2:521385/1‑149 (MQ=255)
                                                                              cAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATaa                                                                     >  1:996069/1‑149 (MQ=255)
                                                                              cAAAGCGCACTTTGTCAAAAATCGAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATaa                                                                     <  2:922620/149‑1 (MQ=255)
                                                                               aaaGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCTACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAACTCAAGCATaaa                                                                    >  2:465855/1‑149 (MQ=255)
                                                                                 aGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATaaaaa                                                                  <  2:604088/149‑1 (MQ=255)
                                                                                    gcACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACGCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCGGCATAAAAAttt                                                               >  1:514631/1‑149 (MQ=255)
                                                                                    gcACTTTGTCAAAAATCTAACCAACTTTTTCATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAAttt                                                               >  1:733572/1‑149 (MQ=255)
                                                                                    gcACTCTGTCATAAATCTACCCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGTCCGTTCCCTACTCGCCCCGAGAAAAATAGCATTAATGCGGCCAGCGCCAGCATAAAAAttt                                                               <  1:1019359/149‑1 (MQ=255)
                                                                                        tttGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTCACTCGCCCAGCGCCAGCATAAAAATTTTGAg                                                           <  2:673014/149‑1 (MQ=255)
                                                                                        tttGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCAAGCGCCAGCATAAAAGTTTTgcg                                                           >  2:1182559/1‑147 (MQ=255)
                                                                                            tCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGCGAAAAATAGCATTAATGCGCCCAACGCCAGCATAAAAATTTTGagaaga                                                       >  2:52106/1‑149 (MQ=255)
                                                                                             cAAAAATCCAACCTCCTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGaa                                                      <  1:442798/149‑1 (MQ=255)
                                                                                                  aTCTAACCTACTTTTTAATTCAGGGATTTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCAGTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTaa                                                 >  1:810039/1‑149 (MQ=255)
                                                                                                    cTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAt                                               <  2:1406828/149‑1 (MQ=255)
                                                                                                     tAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCATGGCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAtt                                              <  2:887066/149‑1 (MQ=255)
                                                                                                       aCCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAttgt                                            >  1:276324/1‑149 (MQ=255)
                                                                                                       aCCTACTTTTTAATTCAGGGAATGACCGCAAAGCCCACGCCATCATACCCAACGGAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCAAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAttgt                                            <  2:492264/149‑1 (MQ=255)
                                                                                                        ccTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAttgtt                                           <  2:1282390/149‑1 (MQ=255)
                                                                                                         cTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAttgttg                                          >  1:1309498/1‑149 (MQ=255)
                                                                                                         cTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAAttgttg                                          >  1:1309488/1‑149 (MQ=255)
                                                                                                           aCTTTTTAATTCAGGTAATTACAGCAAAGCCCACCCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCACCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGc                                        <  2:451287/149‑1 (MQ=255)
                                                                                                           aCTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGc                                        <  2:1247237/149‑1 (MQ=255)
                                                                                                             tttttGATCCAGGGAATTACCGCAAAGCCCACGCCCTCATACCTAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGt                                      <  2:694227/149‑1 (MQ=255)
                                                                                                             aaattAATTCAGGAAATTACCACAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACCCACCCCGAGAAAAATAGCATTAATGCGCCCAAAACCAACAAAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGt                                      <  2:1316618/146‑1 (MQ=255)
                                                                                                                  aaTTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATaa                                 <  1:1192969/149‑1 (MQ=255)
                                                                                                                    ttCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAggg                               >  1:855858/1‑149 (MQ=255)
                                                                                                                         ggAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACACGCCCCGAGAAAAATAGCATTAATGCGCCCCGCGCCCGCATAAAAATTTTAAGCAGACGAAAAATTGTGGCCGTGATaaaggcaag                          >  2:409240/1‑142 (MQ=255)
                                                                                                                             ttACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACaa                      >  1:500773/1‑149 (MQ=255)
                                                                                                                              tACCGCACAGCCCACGCCATCACACCCGACGTAACAAAACTCACGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCGGCGCCAGCATAAAAATGTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACaac                     <  1:576525/149‑2 (MQ=255)
                                                                                                                              tACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACaaa                     <  2:1005324/149‑1 (MQ=255)
                                                                                                                              tACCGCAAAGCCCACGCCATCACACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACaaa                     <  1:576580/149‑1 (MQ=255)
                                                                                                                              tACCGCAAAGCCCACGCCATCACACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACaaa                     <  1:103812/149‑1 (MQ=255)
                                                                                                                              aaCCGCAAACCCCACGCCATCATACCCAACGTAACAAAACTCATGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCAAGCCCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACaaa                     <  2:365489/148‑1 (MQ=255)
                                                                                                                               aCCTCAAAGCCCATGCCATCATACCAAACTTAACAAAACTCAGTCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGACCCGCGCCCGCCTGGACATGTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAAc                    <  2:599765/149‑1 (MQ=255)
                                                                                                                               aCCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAAc                    <  1:223928/149‑1 (MQ=255)
                                                                                                                                catcAAAGACCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCTAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTTGCGTGATAAGGATATTACAAACt                   <  1:875919/146‑1 (MQ=255)
                                                                                                                                 cGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACtt                  <  2:435807/149‑1 (MQ=255)
                                                                                                                                    agaGCCCACGCCCTCATACCCTATGTAACAAGACTCAGGCACGTTCCCCACTCGTCACGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCaa               <  2:264841/147‑1 (MQ=255)
                                                                                                                                    aaaGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCaa               <  2:1399439/149‑1 (MQ=255)
                                                                                                                                       gCCCACGCCATCATACTCAACGTAACCAAACCCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCaataa            <  2:98805/149‑1 (MQ=255)
                                                                                                                                           aCGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCAATAATAcc        >  1:654460/1‑149 (MQ=255)
                                                                                                                                            cGCCATCATACCCAACGTAACAAAACTCAGTCAAGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATAAACCCAGCGACAGCCTAAAAAATATGAGAAGAAGAAAAATTCTAGGCATGATAAAGAAATTCCAAACATCAATAATAACg       >  2:274765/1‑149 (MQ=255)
                                                                                                                                            cGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATGCCGGTATTGCAAACCTCcatcagaacg       >  1:324717/1‑142 (MQ=255)
                                                                                                                                                atcttACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCAATAATACCGGCaa   <  2:1204916/149‑1 (MQ=255)
                                                                                                                                                 tcatACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCAATAATACCGGCAAg  >  1:1158493/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAAAGCGCACTTTGTCAAAAATCTAACCTACTTTTTAATTCAGGGAATTACCGCAAAGCCCACGCCATCATACCCAACGTAACAAAACTCAGGCACGTTCCCCACTCGCCCCGAGAAAAATAGCATTAATGCGCCCAGCGCCAGCATAAAAATTTTGAGCAGAAGGTAAATTGTTGGCGTGATAAGGATATTACAAACTTCAATAATACCGGCAAG  >  NC_000913_3_pae_tpiA/3563607‑3563900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: