Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 4,111,596 G→A 100% L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,111,5960GA100.0% 229.2 / ‑6.7 82L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (48/28);  minor base C (0/3);  total (48/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAT  >  NC_000913_3_pae_tpiA/4111453‑4111740
                                                                                                                                               |                                                                                                                                                
aGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTAAACCCCt                                                                                                                                             >  2:48241/1‑149 (MQ=255)
 ggAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCtt                                                                                                                                            >  2:494863/1‑149 (MQ=255)
          ccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCgaagtt                                                                                                                                   <  1:654795/149‑6 (MQ=255)
          ccgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGtt                                                                                                                                   <  2:1271152/149‑1 (MQ=255)
              cccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTcc                                                                                                                               <  2:1228120/149‑1 (MQ=255)
              cccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGAGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTca                                                                                                                               >  2:536672/1‑148 (MQ=255)
                 gCAGGGCCGAAGGTTTGTGTAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgag                                                                                                                            >  2:1232505/1‑147 (MQ=255)
                     ggCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCTTTTTCGCCGTTGTCCGCGacaa                                                                                                                        <  1:1068689/149‑1 (MQ=255)
                      gcagcaGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGccacc                                                                                                                       >  1:388269/1‑147 (MQ=255)
                      gcagcaGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCCAAGTTGCAGTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGacaac                                                                                                                       <  1:1333409/149‑1 (MQ=255)
                         gcagGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATa                                                                                                                    <  1:1135084/149‑1 (MQ=255)
                              tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCCCCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                               <  2:1150386/149‑1 (MQ=255)
                              tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                               <  2:51403/149‑1 (MQ=255)
                              tCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                               <  1:49165/149‑1 (MQ=255)
                               cTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAg                                                                                                              <  1:168409/149‑1 (MQ=255)
                                  tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGc                                                                                                           >  2:697532/1‑149 (MQ=255)
                                  tgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACACCTTTTCGCCGTTGTCCTCGACAACACAACCCACTGc                                                                                                           >  1:1387837/1‑149 (MQ=255)
                                     aaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAcc                                                                                                        >  2:1406942/1‑149 (MQ=255)
                                         gTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGCTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt                                                                                                    <  2:765918/149‑1 (MQ=255)
                                         gTATAGACGCTCGTCGGCACAAACGCTTTGCTTGTTGTCATTTATTCAAACCTTCACGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCTAAGGTCATCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt                                                                                                    <  2:807585/149‑1 (MQ=255)
                                          tataGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTGAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAATATAACGCAGGGAACCTTCTg                                                                                                   >  1:620812/1‑149 (MQ=255)
                                            taGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATACCGCAGTGCACCTTCTgcc                                                                                                 >  2:1113312/1‑147 (MQ=255)
                                            taGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc                                                                                                 >  1:588044/1‑149 (MQ=255)
                                              gataatCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAAGCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtctc                                                                                               <  2:451741/144‑1 (MQ=255)
                                               agaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCg                                                                                              >  1:1101938/1‑149 (MQ=255)
                                               agaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCg                                                                                              <  1:367355/149‑1 (MQ=255)
                                                  ttCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGgtg                                                                                           >  1:428596/1‑149 (MQ=255)
                                                    cATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGta                                                                                         <  1:991702/149‑1 (MQ=255)
                                                      tCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCCACCCCTTTTCGCCGTTGTCCGCGACAAAATAACGCAGTGCACCTTCTGTCTCGGTGaaat                                                                                       >  1:618404/1‑149 (MQ=255)
                                                            cagaagctttgcagtTATTCATTTGCTCAAACCCTCTGCCGTTTCACATATCGCCAGCCCAATCGGCTCTACAGCGAACGCCAGCCCCTTTTCGCCGTCGGCCGAGACAACATACCGCAGTGCACCTTCTGTCTCGGTGTAATAACgtt                                                                                 <  2:1265916/144‑1 (MQ=255)
                                                             aaaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCATCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgttt                                                                                >  1:279540/1‑149 (MQ=255)
                                                             aaaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgttt                                                                                >  1:187701/1‑149 (MQ=255)
                                                             aaaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgttt                                                                                >  1:1270670/1‑149 (MQ=255)
                                                                   tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATCACGTTTGttttt                                                                          >  2:1182056/1‑149 (MQ=255)
                                                                   tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATCACGTTTGttttt                                                                          >  2:117269/1‑149 (MQ=255)
                                                                    ttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCATCCCGTTTTCGCCGTTGGCCGCGACGACATAGCGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTc                                                                         <  2:1413967/149‑1 (MQ=255)
                                                                    ttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTc                                                                         <  1:928092/149‑1 (MQ=255)
                                                                        tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCCACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTTTCTCGGTGTAATAACGATCGTTTTTCCccg                                                                     >  2:1186047/1‑149 (MQ=255)
                                                                        tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccg                                                                     >  1:500097/1‑149 (MQ=255)
                                                                        tttttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGAGCCAGCTTAATCGGTTCAACAACGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATTACGTTTGTGTTTCCccg                                                                     >  1:485854/1‑149 (MQ=255)
                                                                              tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTta                                                               <  1:447272/149‑1 (MQ=255)
                                                                              tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCCTTTCGCCGTTGTCAGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTta                                                               >  2:1176030/1‑149 (MQ=255)
                                                                                aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagt                                                             >  2:1330533/1‑149 (MQ=255)
                                                                                aTTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCAGTGTAATACCGTTTGTTTTTCCCCTCCGTtagt                                                             >  2:87314/1‑149 (MQ=255)
                                                                                 tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagta                                                            >  1:988890/1‑149 (MQ=255)
                                                                                 tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTtagta                                                            >  1:1274166/1‑149 (MQ=255)
                                                                                   tATTCAAACCTTCCAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCACCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGc                                                          <  1:985499/149‑1 (MQ=255)
                                                                                     ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTACTAACGTTTGTTTTTCCCCTCCGTtagttacga                                                        >  2:687924/1‑144 (MQ=255)
                                                                                     ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCAGCCGTTAGTAACGa                                                        >  2:687944/1‑149 (MQ=255)
                                                                                      tCAAACCATCAAGGGATTCAGATGGCACCAGCTTAATCGCTTCAACCATTAAGGCCAACCCTCTTTCGCCGCTGTCCGCGACAACATAACGCAGTCAACCTCCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                       <  2:60086/149‑1 (MQ=255)
                                                                                        aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCCGTGCACCTTCTTTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTg                                                     >  1:1103349/1‑149 (MQ=255)
                                                                                        aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTg                                                     >  1:1103594/1‑149 (MQ=255)
                                                                                        aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGGAGCGACTg                                                     >  2:702463/1‑149 (MQ=255)
                                                                                        aaaCCTTCAAGCGATTCAGATAGCGCCAACTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAAGGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTg                                                     >  1:780384/1‑149 (MQ=255)
                                                                                            cTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAACGAAGTTCAACGACTTCTTGCCGTGGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTGTTCCCCGCCGTTAGTAGCGACTGCAGt                                                 >  1:1266490/1‑149 (MQ=255)
                                                                                              tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGCAGGTCAACCCCTTTTCGCCGTTGGCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATATCGTTTGTTTTTCCCACCCGTTAGTAGCCACTGCAGtgt                                               >  1:1066720/1‑147 (MQ=255)
                                                                                               cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtatt                                              >  2:786549/1‑149 (MQ=255)
                                                                                                aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                             >  1:1263139/1‑149 (MQ=255)
                                                                                                aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                             >  1:1108338/1‑149 (MQ=255)
                                                                                                aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGttttt                                             >  1:148981/1‑149 (MQ=255)
                                                                                                  gCGATTCAGATAGCGCCAGCTTCATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                           <  1:767648/149‑1 (MQ=255)
                                                                                                  gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                           <  2:988890/149‑1 (MQ=255)
                                                                                                  gCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                           <  1:739849/149‑1 (MQ=255)
                                                                                                   cGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCt                                          <  1:988631/149‑1 (MQ=255)
                                                                                                           aTAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                  <  1:612870/149‑1 (MQ=255)
                                                                                                                  cAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTgg                           >  2:1167257/1‑149 (MQ=255)
                                                                                                                      ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGGGTTTTGGTCAt                       >  1:410888/1‑149 (MQ=255)
                                                                                                                      ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGAGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                       >  2:105808/1‑149 (MQ=255)
                                                                                                                           cGGGTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATg                  <  1:353305/149‑1 (MQ=255)
                                                                                                                            ggTTCAAAAGCGAAGGTCACCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgg                 <  1:358377/149‑1 (MQ=255)
                                                                                                                            ggTTAAACAGCGAAGGTCAACCCCTTTCCGCCGTTTTCCTCGACAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgg                 <  2:1105003/149‑1 (MQ=255)
                                                                                                                             gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                >  1:1201697/1‑149 (MQ=255)
                                                                                                                              ttCCACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGGCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCACTAATgggg               <  1:27097/149‑1 (MQ=255)
                                                                                                                               tCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt              <  1:1077091/149‑1 (MQ=255)
                                                                                                                                 aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTTGCGACTGCAGGTTTTTCTGGCTTTGTGTTTTGGTCATTAATggggcgc            >  2:420874/1‑146 (MQ=255)
                                                                                                                                 aaCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa            <  1:1087839/149‑1 (MQ=255)
                                                                                                                                  aCAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg           <  1:697532/149‑1 (MQ=255)
                                                                                                                                     gCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCATCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTATTCTGCCTTTGTGTTTTGGTCATTAATGGGGTGCgtgt        >  2:778353/1‑149 (MQ=255)
                                                                                                                                      cGAAGGTCAACCCCTTTTCGCCTTTTTCCGCGACAACATAACGGAGTGCACCTGCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTATTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGtgtg       <  1:1356241/149‑1 (MQ=255)
                                                                                                                                      cGAAGGTCAACCCCTTTTCGCCGTTGTCTGCGACAACGTAACGCACTGCACCTTCTGTCCCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTGTGTGTTTTGGTCATTAATGGCGTGCAtgtg       >  1:1048121/1‑149 (MQ=255)
                                                                                                                                          ggTCAACCCCTTTTCGCCGTTGTTCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGa   >  1:1241434/1‑149 (MQ=255)
                                                                                                                                           gTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt  >  1:102814/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAT  >  NC_000913_3_pae_tpiA/4111453‑4111740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑