Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 349,642 A→G 9.1% intergenic (+70/‑370) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA349,6420AG 9.1% 226.3 / 10.4 93intergenic (+70/‑370)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (43/40);  new base G (5/4);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGA  >  NC_000913_3_pae_tpiA/349495‑349789
                                                                                                                                                   |                                                                                                                                                   
tacctctACGAAAGCATCAACTACTACCGTGACGAAGAGAAGGCCGACATCTTGTTTGACCGTAGCCAGGTGAAATAAAAAGCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGGCGCTTGCGCGACTTATCAGGCCTACGAg                                                                                                                                                    <  2:1012882/143‑1 (MQ=255)
  gCTGTACGAAAGCATCACCTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGt                                                                                                                                                  <  2:1044971/149‑1 (MQ=255)
   cTGTACGAAAGCATCAATATCTTCCAGTACGAAGCTAAGCTCGACAGGTTGTCTGCCGGTGGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTAAGAGGTg                                                                                                                                                 <  2:663211/149‑1 (MQ=37)
   cTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGCCGCGACTTATCCGGCCTACGAGGTg                                                                                                                                                 >  1:1418425/1‑149 (MQ=255)
    tGTACGACAGCATCAACTACTACCAGAACTAAGAGAAGCTCGACACCCTGTTTGCCCGTAGCCAGGTGAAATAACCAACACCCGCTGATTGTATTCGACACCCGATGCCTGATACATCGCTGACGCGACTTATCAGGCCCACGAGCTGc                                                                                                                                                >  2:98290/1‑149 (MQ=255)
    tGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGc                                                                                                                                                >  2:530736/1‑149 (MQ=255)
    tGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGAGGTGc                                                                                                                                                >  1:20905/1‑149 (MQ=255)
    tGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTAGACAGCAGATGCCTGATGCGCCGCTGCCGTGCCTTATCAGGCCTACGGGGTGc                                                                                                                                                >  2:149729/1‑149 (MQ=38)
       aCGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCATTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACt                                                                                                                                             <  1:982264/149‑1 (MQ=255)
         gAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGa                                                                                                                                           >  1:1198526/1‑149 (MQ=255)
           aaGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAAc                                                                                                                                         <  2:954188/149‑1 (MQ=255)
              cATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGt                                                                                                                                      >  1:128513/1‑149 (MQ=255)
              cATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGt                                                                                                                                      >  1:1414354/1‑149 (MQ=255)
                tCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAg                                                                                                                                    >  1:589981/1‑149 (MQ=255)
                tCAAATACAACCAGGACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAg                                                                                                                                    <  2:174311/149‑1 (MQ=255)
                 cAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCACGTTGATTGTATTCGACAACCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAgg                                                                                                                                   >  2:1219286/1‑149 (MQ=255)
                   actactACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTc                                                                                                                                 >  2:499712/1‑149 (MQ=255)
                     tactacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCgg                                                                                                                               >  1:506016/1‑149 (MQ=255)
                      actacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                                                                                                              <  1:1057764/149‑1 (MQ=255)
                       ctacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGAt                                                                                                                             >  2:1257171/1‑149 (MQ=255)
                       ctacCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGAt                                                                                                                             >  1:746374/1‑149 (MQ=255)
                        ttccAGTACGAATAGAAGCTCGACAACCTGTTTGCCCGTAGCCAAGTGAAATAAAAGACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATa                                                                                                                            <  1:1274419/147‑1 (MQ=25)
                         acCAGTACGGAGCGAAGCTCGGCCACCGGTGTGCCCGTAGCCCGGTGCAATAACAAATGCTTGCTGATGGTATTCGCCAGCCGATGCCCGATGCGTCGCTGACGCGACTTATCAGGCTTACGAGGTGCACTGAACTGTAGGTCGGATaa                                                                                                                           <  2:866968/149‑1 (MQ=18)
                           cAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                                         >  2:1329742/1‑149 (MQ=255)
                            aGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGATGACTCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGAc                                                                                                                        <  2:948848/149‑1 (MQ=255)
                              tACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACgg                                                                                                                      <  2:500424/149‑1 (MQ=255)
                               aCGAAGAGAAGCTCGACAACCGGTTTGACCGTAGGCAGGTTAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCATGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGa                                                                                                                     <  2:385263/149‑1 (MQ=25)
                                cGAAGAGAAGCTCGACAACCTGGTTGCCCGTAGCCAGGTGAAATAAACCACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt                                                                                                                    <  1:894442/149‑1 (MQ=255)
                                    gaagaGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAACACCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTTACGCGACTTATCAGGCCTACGAGGCGCACTGAACGGGTGGTCGGATAAGACGGATGGCg                                                                                                                <  2:1011829/145‑1 (MQ=25)
                                      gaAGCTAGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTAATGCGTCGCTGACGCGACTTATCAGGCCTAAGAGGTGCACAGAACTGTAGGTCGGATAAGACGGATAGCGTc                                                                                                              >  2:21415/1‑149 (MQ=25)
                                          cTCGACAACCTGTTTGCCCGTAGACAAGTGAAATAAAAACCGCCCGTTGATTGTAGTCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGGCTACGTGGTGCACGGAACGGTAGGTCGGATAACCCGGATGGCGGCGCac                                                                                                          >  1:817976/1‑148 (MQ=21)
                                           tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATc                                                                                                         >  1:1315697/1‑149 (MQ=255)
                                           tCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCACGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATc                                                                                                         >  2:238899/1‑149 (MQ=255)
                                             gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCg                                                                                                       >  2:1332712/1‑149 (MQ=255)
                                             gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCg                                                                                                       >  1:857249/1‑149 (MQ=255)
                                              aCAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                                                      >  1:127450/1‑149 (MQ=18)
                                                aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACa                                                                                                    >  1:1324298/1‑149 (MQ=255)
                                                aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACa                                                                                                    >  2:1294924/1‑149 (MQ=255)
                                                  ccTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTCCGGGGTGCACTGAACTGTAGGACGGATAAGACGCATAGCGTCGCATCCGCCAAc                                                                                                  >  2:576858/1‑149 (MQ=18)
                                                  ccTGATTGCCCGGAGCCAGGTGAAATAAAAACCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAAc                                                                                                  <  1:1234362/149‑1 (MQ=255)
                                                   cTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAAcc                                                                                                 >  1:167097/1‑149 (MQ=255)
                                                      tttGCCCGTAGCCAGGTGAAATAAAAAGCGCCCGTTGATTGTATTCGACAGTCGATGCCTGATGCGTCGCTGACGCGACTTATCATGCCTACGAGGTGCACTCAACGGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGAt                                                                                              <  1:123303/149‑1 (MQ=25)
                                                        tGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGc                                                                                            <  2:490408/149‑1 (MQ=255)
                                                          cccGTAGCCAGGTGAAATAAAACACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGTGTCGCATCCGACAACCGATGCCt                                                                                          <  1:879905/149‑1 (MQ=255)
                                                          cccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGTGTCGCATCCGACAACCGATGCCt                                                                                          <  1:880020/149‑1 (MQ=255)
                                                           ccGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTg                                                                                         >  2:666786/1‑149 (MQ=255)
                                                            cGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGACGCTAACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCAAACAACCGAttcgtgc                                                                                        >  2:1072570/1‑143 (MQ=21)
                                                              tAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                                                      >  2:296509/1‑149 (MQ=14)
                                                                 ccAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                                                   <  1:855672/149‑1 (MQ=255)
                                                                   aGGTTAAAAAAAAAACGCCCGTTGATTGGATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                                                 <  1:1080958/149‑1 (MQ=255)
                                                                    ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                                                <  1:1208755/149‑1 (MQ=255)
                                                                    ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                                                >  1:652457/1‑149 (MQ=255)
                                                                      tGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTg                                                                              >  1:1073964/1‑149 (MQ=14)
                                                                       gAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                             >  1:581647/1‑149 (MQ=255)
                                                                        aaaTAAAGAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAc                                                                            <  2:1316420/149‑1 (MQ=255)
                                                                         aaTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACg                                                                           <  1:1112722/149‑1 (MQ=255)
                                                                           tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTg                                                                         >  2:337192/1‑149 (MQ=255)
                                                                            aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                        <  2:1363073/149‑1 (MQ=255)
                                                                             aaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATAGCGTCGCATCCGACAACCAATGCCTGATGCGACGgtgacgtgac                                                                       >  2:338048/1‑149 (MQ=255)
                                                                              aaaaCGCTCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGTGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACt                                                                      <  1:97302/149‑1 (MQ=255)
                                                                                   gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                                         <  1:106641/93‑1 (MQ=255)
                                                                                   gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                                         >  2:106641/1‑93 (MQ=255)
                                                                                   gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                 >  1:1095053/1‑149 (MQ=255)
                                                                                   gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                 <  1:551731/149‑1 (MQ=255)
                                                                                   gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa                                                                 <  2:1234441/149‑1 (MQ=255)
                                                                                      cGTTGCTTGTATTCTACAGCCGATGCCTGATGAGTAGGTGACGCGCACTATATGCGCTGCGGCGGGCACTGAACTGTAGGTCGGAGAGGACCGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGc                                                              <  2:1002077/149‑1 (MQ=14)
                                                                                       gTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGcc                                                             >  1:1295488/1‑149 (MQ=255)
                                                                                        ttGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGCCTTATCAGGCCt                                                            >  1:1353464/1‑149 (MQ=255)
                                                                                           aTTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCTGCAACCGTTGTCTTGTGCGCCTTTTACTTGACTTAGCATGCCTACg                                                         >  1:660195/1‑149 (MQ=17)
                                                                                            ttGTATTCGAGAGCCGATGCCTGGTGCGTCGCTGACGCGACTTATCAGGCCTAGGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg                                                        <  2:147336/149‑1 (MQ=255)
                                                                                            ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGTAGTGACTTATCAGACCTACgg                                                        >  2:427304/1‑149 (MQ=255)
                                                                                              gTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGCCAACCGATGCCTGATGCGCCGCTGCCGGGACTTTTCAGGCCCACgggg                                                      >  2:255941/1‑149 (MQ=17)
                                                                                                  tCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                                                  <  1:1133464/149‑1 (MQ=255)
                                                                                                  tCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCa                                                  >  1:231970/1‑149 (MQ=255)
                                                                                                    gACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACt                                                >  1:650808/1‑149 (MQ=255)
                                                                                                       aaccGATGCCTGGTGCGTCGCTGACACGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                             <  2:608626/147‑1 (MQ=255)
                                                                                                          cGATGCCTGATGCGTCGCTGACGCGNCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTg                                          <  1:934166/149‑1 (MQ=255)
                                                                                                            aTGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGa                                                                                                                         >  2:455057/1‑68 (MQ=255)
                                                                                                             tGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg                                       <  1:573904/149‑1 (MQ=255)
                                                                                                                ctggtccGTCGCAGACGCGACTTATCAGGCCTACGAGGGGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTc                                    <  2:1418425/143‑1 (MQ=255)
                                                                                                                 tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACt                                                                                                                                             >  1:953417/1‑43 (MQ=255)
                                                                                                                 tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACt                                                                                                                                             <  2:953417/43‑1 (MQ=255)
                                                                                                                 tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCGGACGCGACTTATCAGGCCTAACGGGTGCCCTGAACTGTAGGTCg                                   >  2:170191/1‑149 (MQ=14)
                                                                                                                   aTGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa                                 <  1:1257171/149‑1 (MQ=255)
                                                                                                                      cGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa                              <  2:1396517/149‑1 (MQ=255)
                                                                                                                           cTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGc                         <  2:1414354/149‑1 (MQ=255)
                                                                                                                           cTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGCGCACTGAACTGTAGGTCGGATAAGACGc                         >  2:255030/1‑149 (MQ=255)
                                                                                                                                 cgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGACCTGTAGGTCGGATAAGACGCATAGCg                   >  1:788079/1‑149 (MQ=255)
                                                                                                                                   aCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                 <  1:127106/149‑1 (MQ=40)
                                                                                                                                               tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc     >  1:794699/1‑149 (MQ=255)
                                                                                                                                                  gAGGTGCACTGAACTGTAGGTCTAATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa  <  2:1269645/149‑1 (MQ=255)
                                                                                                                                                  gAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa  <  2:167097/149‑1 (MQ=18)
                                                                                                                                                  gAGGGGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAGCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGa  <  2:1194788/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAGCTGTACGAAAGCATCAACTACTACCAGTACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGA  >  NC_000913_3_pae_tpiA/349495‑349789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑