Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 564,303 G→A 6.2% P60S (CCC→TCC)  sfmZ ← response regulator family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA564,3030GA 6.2% 221.7 / 2.7 78P60S (CCC→TCC) sfmZresponse regulator family protein
Reads supporting (aligned to +/- strand):  ref base G (38/34);  new base A (2/3);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGAGTA  >  NC_000913_3_pae_tpiA/564155‑564451
                                                                                                                                                    |                                                                                                                                                    
tCATTGCATTTACCGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCggg                                                                                                                                                      <  2:564559/149‑1 (MQ=255)
   ttGCATTTACTTACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTTATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCaa                                                                                                                                                   <  2:375493/149‑1 (MQ=255)
   ttGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCaa                                                                                                                                                   >  1:925126/1‑149 (MQ=255)
   ttGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCaa                                                                                                                                                   >  1:968313/1‑149 (MQ=255)
    tGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAg                                                                                                                                                  >  1:479955/1‑149 (MQ=255)
       aTTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCt                                                                                                                                               >  2:1243387/1‑149 (MQ=255)
       aTTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCt                                                                                                                                               >  2:399075/1‑149 (MQ=255)
        tttACTGACAAAACCGTCAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCta                                                                                                                                              <  1:406751/149‑1 (MQ=255)
                   aaCCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATg                                                                                                                                   <  2:1416945/149‑1 (MQ=255)
                        ttAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGCttca                                                                                                                              >  1:256865/1‑147 (MQ=255)
                        tgaGCGCCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCTATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTaa                                                                                                                              <  2:356590/147‑1 (MQ=255)
                           gCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATc                                                                                                                           >  2:65054/1‑149 (MQ=255)
                           gCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATc                                                                                                                           >  2:64776/1‑149 (MQ=255)
                            cACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCa                                                                                                                          >  2:710613/1‑149 (MQ=255)
                              ccAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAAc                                                                                                                        <  2:354061/149‑1 (MQ=255)
                              ccAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAAc                                                                                                                        <  1:404309/149‑1 (MQ=255)
                               cAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAACACCGTCTGCTCCGGGGAAGTCTATATCCATCATGCTTAAATCAACa                                                                                                                       >  2:832295/1‑149 (MQ=255)
                               cAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCGAGTCTATATCCATAATGATTAAATCAACa                                                                                                                       <  2:1226035/149‑1 (MQ=255)
                               cAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACa                                                                                                                       >  2:392073/1‑149 (MQ=255)
                                aGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAg                                                                                                                      <  2:523142/149‑1 (MQ=255)
                                aGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAg                                                                                                                      >  2:553428/1‑149 (MQ=255)
                                aGCTTGTATCGCTCTGCCAGCATAAAAGCATTATGATTTCTATGTTAAAACTAACACTTTCTCTGTTTTCTGGATTTGTTTGAACATTTTCAGGAAGGTAAAACCGTCTGTTCCGTGCAAGTCTATATCAATAATTATTAAGTCATCAg                                                                                                                      >  2:875262/1‑149 (MQ=255)
                                  cTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGa                                                                                                                    <  1:994007/149‑1 (MQ=255)
                                  cTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGa                                                                                                                    <  1:993941/149‑1 (MQ=255)
                                  cTTGTATCGCTCTGCCAGCATAAAAGCACTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATAACCATAATGATTAAATCAACAGGa                                                                                                                    <  1:1235308/149‑1 (MQ=255)
                                    tGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACg                                                                                                                  >  2:15957/1‑149 (MQ=255)
                                     gTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACgg                                                                                                                 <  1:495954/149‑1 (MQ=255)
                                        tCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGACAAGTCTATATCCATAATGATTAAATCAACAGGACGGGtt                                                                                                              >  1:503663/1‑149 (MQ=255)
                                        tCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGACAAGTCTATATCCATAATGATTAAATCAACAGGACGGGtt                                                                                                              >  1:503531/1‑149 (MQ=255)
                                            tctGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGga                                                                                                          <  2:609842/149‑1 (MQ=255)
                                            tctGCCAGCAGAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCGCTGTGCGCTGGATTTGTTGGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGga                                                                                                          <  2:1222236/149‑1 (MQ=255)
                                               gCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGAt                                                                                                       <  2:1377559/149‑1 (MQ=255)
                                               gCCAGCATAAAAGCATTCTGATTTAGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGAt                                                                                                       <  2:713285/149‑1 (MQ=255)
                                                  aGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCGATATCCATATTGATTAAATCAACAGGACGGGTTCGGAGATAAt                                                                                                    >  1:72273/1‑149 (MQ=255)
                                                     aTAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGa                                                                                                 <  2:399058/149‑1 (MQ=255)
                                                     aTAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGACAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCCTAATGATTAAATCAACAGGACGGGTTCGGAGATGATAGa                                                                                                 >  2:401819/1‑149 (MQ=255)
                                                      tAAAAGCATTCTGATTTCGATGATGAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACATGACGGGTTCGGAGATAATCGAt                                                                                                >  2:374864/1‑149 (MQ=255)
                                                       aaaaGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATg                                                                                               <  2:114481/149‑1 (MQ=255)
                                                        aaaGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGACTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCAATgg                                                                                              >  2:153621/1‑149 (MQ=255)
                                                        aaaGCATTCTGATGTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGGCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATgg                                                                                              <  1:364710/149‑1 (MQ=255)
                                                        aaaGCATTCGGATTTCGATGATAAAAATAACCCTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCAGAATGATTAAATCAACAGGACGGGTTCAGAGATAATCGATgg                                                                                              <  1:364230/149‑1 (MQ=255)
                                                           gCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGTATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTa                                                                                           <  2:6589/149‑1 (MQ=255)
                                                                 tGATTTCGATGATAAAAATAACACTTTCCCTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAAAAGGACGGGTTCGGAAATAATCGAAGGATATGCAAt                                                                                     >  2:26446/1‑149 (MQ=255)
                                                                   aTTTCGATGATAAAAATAACACTTTCACTGTGCTGTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATaa                                                                                   >  2:1150732/1‑149 (MQ=255)
                                                                   aTTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGTTTCGGAGATACTCGCTGGTTATGCGATaa                                                                                   >  2:559072/1‑149 (MQ=255)
                                                                      tCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAatcc                                                                                >  2:865021/1‑148 (MQ=255)
                                                                        gatgatAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAatcatc                                                                              <  1:399075/149‑1 (MQ=255)
                                                                        gatgatAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAatcatc                                                                              >  2:187822/1‑149 (MQ=255)
                                                                         atgatAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGAGTTCGGAGATAATCGATGGTTATGCGATAATCATcc                                                                             >  2:542021/1‑149 (MQ=255)
                                                                             tAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGttt                                                                         >  2:812130/1‑149 (MQ=255)
                                                                                   tAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATCATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGa                                                                   >  2:363646/1‑149 (MQ=255)
                                                                                          ttCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAAGGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGGTATGCGATAATCATCCGTTTTCAGGACAATCTg                                                            <  1:1161934/149‑1 (MQ=255)
                                                                                             aCTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCaa                                                         >  1:1266862/1‑149 (MQ=255)
                                                                                                    tctGGATTTGTTTGATCCTTTTCAGGAAGTTAGAACCGGCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTg                                                  <  1:1252287/149‑1 (MQ=255)
                                                                                                      tGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCtata                                                                                                                                            >  1:1048787/1‑57 (MQ=255)
                                                                                                      tGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCtata                                                                                                                                            <  2:1048787/57‑1 (MQ=255)
                                                                                                        gATTTGCTTGATCCTTTT‑AGAAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGttttt                                             <  1:243527/149‑1 (MQ=255)
                                                                                                           ttgtttgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGttttttt                                           <  1:1122147/149‑1 (MQ=255)
                                                                                                            tgtttgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTGACTGTTTTTTTg                                          >  1:185128/1‑149 (MQ=255)
                                                                                                            tgtttgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGACAATTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATTAGCCTTGCTCAGGACAATCTGCAATTCACTGTTTTTTTg                                          <  1:707205/149‑1 (MQ=255)
                                                                                                               ttgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCaa                                       <  1:75002/149‑1 (MQ=255)
                                                                                                               ttgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGACAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCaa                                       <  2:503531/149‑1 (MQ=255)
                                                                                                               ttgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGACAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCaa                                       <  2:503663/149‑1 (MQ=255)
                                                                                                                tgATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAAc                                      >  1:1125390/1‑149 (MQ=255)
                                                                                                                  aTCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAg                                    <  2:1332507/149‑1 (MQ=255)
                                                                                                                   tCCTTTTCAGGCAGGTAAAACCGTCTGTTACATTTGAGTGGATATCCATAATGATTAAATCAGCAGGACGGGTGCGGAGATAATCTATGGTTATGCGATAATCATCCGTTTTCAGGACAAACTGCAACTAACTGATATTTTGCAACAGa                                   >  2:671936/1‑149 (MQ=255)
                                                                                                                         tCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCa                             <  1:460281/149‑1 (MQ=255)
                                                                                                                              aaGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCACCAGCACTTCAATAGa                        >  1:545023/1‑149 (MQ=255)
                                                                                                                                ggTAAAACCGTCTGTTCCGGGCAAGGCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACa                      >  1:1117375/1‑149 (MQ=255)
                                                                                                                                  tAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTATCAGGACCATTTTTTTTTTTTTTTTTTTTTGCAACAGACCTTCACTAGACAtt                    >  2:1360781/1‑149 (MQ=255)
                                                                                                                                      actgTCTTTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTGTTTGGTGCAGAACTTCAATAGACATTCtga                <  2:1011691/146‑1 (MQ=255)
                                                                                                                                       ccGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCa                                                                      >  2:804870/1‑94 (MQ=255)
                                                                                                                                       ccGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCa                                                                      <  1:804870/94‑1 (MQ=255)
                                                                                                                                       ccGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCttat               >  2:468168/1‑149 (MQ=255)
                                                                                                                                          tCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGTTACTTATCTTCAATAGACATTCTgatgat            >  1:382014/1‑149 (MQ=255)
                                                                                                                                          tCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGAAATTCTgatgat            >  2:1042839/1‑149 (MQ=255)
                                                                                                                                              ttCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCACCAGAACTTCAATAGACATTCTTATGATAGGa        >  2:1195690/1‑149 (MQ=255)
                                                                                                                                                 cGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGa     <  1:65054/149‑1 (MQ=255)
                                                                                                                                                 cGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGa     <  1:64776/149‑1 (MQ=255)
                                                                                                                                                  gggCAAGTTTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGAg    <  2:540250/149‑1 (MQ=255)
                                                                                                                                                  gggCAAGTCTATATCCATAATGATTAAATCAACAGGTCGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGAg    <  2:1273061/149‑1 (MQ=255)
                                                                                                                                                    gCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGGTCGGAGCTGATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGAGTa  <  1:553428/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCTCTGGATTTGTTTGATCCTTTTCAGGAAGGTAAAACCGTCTGTTCCGGGCAAGTCTATATCCATAATGATTAAATCAACAGGACGGGTTCGGAGATAATCGATGGTTATGCGATAATCATCCGTTTTCAGGACAATCTGCAATTCACTGTTTTTTTGCAACAGAACTTCAATAGACATTCTGATGATAGGATGAGTA  >  NC_000913_3_pae_tpiA/564155‑564451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑