New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 225456 =10 (0.080)25 (0.220) 21/242 9.7 72.9% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913_3_pae_tpiA = 3427056 NA (NA)intergenic (‑23/+20) alaU/ileU tRNA‑Ala/tRNA‑Ile
Rejected: Coverage evenness skew score above cutoff.

GCAGGTACACTCTGAAGTATTTTTTATTTAATCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTT  >  NC_000913_3_pae_tpiA/3426915‑3427175
                                                                                                                                             |                                                                                                                       
gcgggTGCACTCTGAAGTATTTTTTATTTAAGCACTACAGATATGGTGGAGCTATGCCGGATCGAACCGCAGACCTCCCGCGTGCAAAGCAGGCGCTCTCACAGCTGAGCTATAGCCCCATACCATGTAGTTACAACCTCTTCGAATTTg                                                                                                                 <  4:667105/147‑1 (MQ=1)
                                                                                                               atggCCCTATAACATGTAGTTAAAACCTCTTCAAATTTGGCGTGCACATTTGGTAGGCCTGAGTGGACTTAAACCACAGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCAtttt  >  2:480325/4‑150 (MQ=1)
                                                                                                                                             |                                                                                                                       
GCAGGTACACTCTGAAGTATTTTTTATTTAATCACTACAGAGATGGTGGAGCTATGCGGGATCGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGAGCTACAAGCCTGTAGAGGTTTTACTGCTCATTTT  >  NC_000913_3_pae_tpiA/3426915‑3427175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.