New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 225456 =8 (0.080)23 (0.250) 20/222 7.1 75.7% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913_3_pae_tpiA = 3427056 NA (NA)intergenic (‑23/+20) alaU/ileU tRNA‑Ala/tRNA‑Ile
Rejected: Coverage evenness skew score above cutoff.

AAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT  >  NC_000913_3_pae_tpiA/3427000‑3427076
                                                        |                     
aaaGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACGGCAAAttt  >  1:934399/1‑77 (MQ=1)
aaaGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACGGCAAAttt  <  2:934399/77‑1 (MQ=1)
                                                        |                     
AAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCCGTGCAAATTT  >  NC_000913_3_pae_tpiA/3427000‑3427076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.