New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	3173468 =	54 (1.030)	6 (0.120)	6/266	6.2	10.3%	intergenic (+12/+36)	ygiN/parE	quinol monooxygenase/DNA topoisomerase IV, subunit B
	?	NC_000913_3_pae_tpiA	3173493 =	54 (1.090)					intergenic (+37/+11)	ygiN/parE	quinol monooxygenase/DNA topoisomerase IV, subunit B
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATGATGAAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/3173622‑3173468 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgggcaaTGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCT  >  NC_000913_3_pae_tpiA/3173493‑3173615                                                                                                                                                                                                                                                                                        ATGATGAAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCTGGCGAAGGAACGCTCGGAAGATCGCCGCAACTGGTTGCATGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCC                                                                                                                                   >  2:839913/1‑149   GATGAAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCGGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGG                                                                                                                                 <  2:451746/149‑1       AAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAA                                                                                                                             <  2:443776/149‑1                               TGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGC                                                                                                                                    <  1:547603/118‑1                               TGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGC                                                                                                                                    >  2:547603/1‑118                                                           CTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACC                                                                                                           >  1:38223/1‑115                                                           CTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACC                                                                                                           <  2:38223/115‑1                                                                                                                  GTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCT                                                                                >  1:140387/1‑87                                                                                                                  GTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCT                                                                                <  2:140387/87‑1                                                                                                                   TTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCACTCTCCGCCATGTCGCCTTTCTCTGGCAACCAGTTGCGGCGATCTTCCGAGCGTGTCTTCCCCAGCGGCATATCAATCATCGCGTCA                 >  2:39862/1‑149                                                                                                                                  ATTGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCCTCGCCAGCAGCATATCCATCATCGCGTCAGTGCGCTGATCGTCT  >  1:576149/1‑149                                                                                                                                                                                                                                                                                        ATGATGAAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAGATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAAAGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/3173622‑3173468 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgggcaaTGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCT  >  NC_000913_3_pae_tpiA/3173493‑3173615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.