New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 3587430 =59 (1.120)7 (0.140) 7/262 5.7 11.0% coding (684/744 nt) ugpQ glycerophosphodiester phosphodiesterase, cytosolic
?NC_000913_3_pae_tpiA 3587464 = 59 (1.210)coding (650/744 nt) ugpQ glycerophosphodiester phosphodiesterase, cytosolic
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAAGACGCCGGACTGCGGATTCTGGTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/3587585‑3587430
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgctggggtTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCA  >  NC_000913_3_pae_tpiA/3587464‑3587588
                                                                                                                                                                                                                                                                                         
CGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAAGACGCCGGACTGCGGATTCTGGTTTATACCGTCAATAAACCACAGCGCGCAGCAGAGGTGCTGCG                                                                                                                                      >  2:431119/1‑149
  CGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAAGACGCCGGACTGCGGATTCTGGTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCT                                                                                                                                    <  1:31093/149‑1
  CGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAAGACGCCGGACTGCGGATTCTGGTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCT                                                                                                                                    <  1:738222/149‑1
     ACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAAGACGCCGGACTGCGGATTCTGGTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGG                                                                                                                                 <  1:430093/149‑1
                                                                                                                           CCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCA                                                                                                   >  1:555644/1‑61
                                                                                                                           CCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCA                                                                                                   <  2:555644/61‑1
                                                                                                                             CCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTT         >  1:514093/1‑149
                                                                                                                              CAGCGCGCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGC                      <  1:899281/135‑1
                                                                                                                              CAGCGCGCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGC                      >  2:899281/1‑135
                                                                                                                                    GCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCA  >  1:121919/1‑149
                                                                                                                                    GCAGCAGAGTTGCTGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCA  <  2:514093/149‑1
                                                                                                                                                                                                                                                                                         
CGCGAACTGACCGCGCGGCTGGGCTGCGTCTCTATTCATCTCAATCATAAGTTACTCAATAAAGCGCGAGTGATGCAGTTGAAAGACGCCGGACTGCGGATTCTGGTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/3587585‑3587430
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgctggggtTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCA  >  NC_000913_3_pae_tpiA/3587464‑3587588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.