New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	2305067 =	156 (1.400)	22 (0.230)	18/224	8.3	15.0%	noncoding (651/655 nt)	REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences	REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences
	?	NC_000913_3_pae_tpiA	2305106 =	115 (1.200)					intergenic (+713/+2)	eco/mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCAGTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/2305228‑2305067 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atgccggatgtggcgtatCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATC  >  NC_000913_3_pae_tpiA/2305106‑2305254                                                                                                                                                                                                                                                                                                                         GCAGTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGTAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACAAGATAAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCG                                                                                                                                                                   <  4:752497/150‑1  CAGTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGG                                                                                                                                                                  >  1:773882/1‑150   AGTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGA                                                                                                                                                                 <  1:1269588/150‑1    GTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGAT                                                                                                                                                                >  4:363948/1‑150    GTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGAT                                                                                                                                                                <  2:773882/150‑1     TACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATG                                                                                                                                                               >  2:35407/1‑150      ACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGT                                                                                                                                                              <  2:739102/150‑1       CACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTG                                                                                                                                                             >  1:219931/1‑150       CACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTG                                                                                                                                                             >  3:153812/1‑150        ACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAGAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGG                                                                                                                                                            <  3:183211/150‑1          CAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCG                                                                                                                                                          >  1:894643/1‑150           AGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGT                                                                                                                                                         >  4:416866/1‑150           AGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGT                                                                                                                                                         >  1:508585/1‑150                         GTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAAGGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCA                                                                                                                                           <  4:709115/150‑1                              ACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGG                                                                                                                                                            <  2:650082/128‑1                              ACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGG                                                                                                                                                            >  1:650082/1‑128                                          CGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGAT                                                                                                                                                                >  1:867361/1‑112                                          CGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGAT                                                                                                                                                                <  2:867361/112‑1                                               GCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGG                                                                                                                                                                  >  1:1213067/1‑105                                               GCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGG                                                                                                                                                                  <  2:1213067/105‑1                                                                          TTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGG                                                                                                                                                                  <  1:603355/78‑1                                                                          TTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGG                                                                                                                                                                  >  2:603355/1‑78                                                                                              AAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGC                                                                      >  1:335796/1‑150                                                                                                    AGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTG                                                                <  2:335796/150‑1                                                                                                                    TTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGC                                                                                        <  2:546549/110‑1                                                                                                                    TTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGC                                                                                        >  1:546549/1‑110                                                                                                                                GCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCA                                             <  2:705169/141‑1                                                                                                                                GCCACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCATGCGGCTTGTCGTAGTTCAACCCCAGCA                                             >  1:705169/1‑141                                                                                                                                   ACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTAT                                                                                 <  4:382310/102‑1                                                                                                                                   ACATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTAT                                                                                 >  3:382310/1‑102                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTAC                               >  1:690183/1‑150                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTG                                  <  2:567756/147‑1                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTG                                  >  1:567756/1‑147                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCA                                                >  4:204118/1‑133                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCA                                                <  3:204118/133‑1                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTC                                                                                                       <  2:957035/78‑1                                                                                                                                     ATCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTC                                                                                                       >  1:957035/1‑78                                                                                                                                      TCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTC                                                                                           <  1:812676/89‑1                                                                                                                                      TCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTC                                                                                           >  2:812676/1‑89                                                                                                                                      TCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGC                                                                                                                                   <  2:977592/49‑1                                                                                                                                      TCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGC                                                                                                                                   >  1:977592/1‑49                                                                                                                                           CATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCT                                                                            <  3:530595/99‑1                                                                                                                                           CATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCT                                                                            >  4:530595/1‑99                                                                                                                                                  TGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAGCTCGCGTT                  >  4:648753/1‑150                                                                                                                                                    CCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCT                >  2:782857/1‑150                                                                                                                                                    CCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCT                <  1:125642/150‑1                                                                                                                                                       gcTGTTGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTT             <  4:582524/148‑1                                                                                                                                                       GATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTT             <  4:582470/150‑1                                                                                                                                                        ATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTG            >  1:1103756/1‑150                                                                                                                                                             GCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCT       >  2:539354/1‑150                                                                                                                                                                TATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCA                                                >  3:370510/1‑106                                                                                                                                                                TATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCA                                                <  4:370510/106‑1                                                                                                                                                                  TCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATC  >  3:664334/1‑150                                                                                                                                                                                                                                                                                                                         GCAGTACACCAGCGAAGTGCTGGGGTTGAACTACGACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAAAAAGAAGTGGCGGATATTGCGTTGTAATGATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/2305228‑2305067 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atgccggatgtggcgtatCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATC  >  NC_000913_3_pae_tpiA/2305106‑2305254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.