BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,756,693	C→A	C332* (TGC→TGA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,756,693	0	C	A	98.5%	472.3 / ‑2.7	137	C332* (TGC→TGA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base C (1/0); major base A (70/64); minor base G (1/1); total (72/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

Only 100 of 138 total aligned reads displayed.

CATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGC  >  NC_000913_3_pae_tpiA/1756546‑1756837

cATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTgaga                                                                                                                                                >  4:433719/1‑150 (MQ=255)

  tGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAAc                                                                                                                                              <  2:1878549/150‑1 (MQ=255)

   gATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACg                                                                                                                                             >  1:154179/1‑150 (MQ=255)

      cAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATTCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTac                                                                                                                                          >  2:2030728/1‑150 (MQ=255)

      cAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTac                                                                                                                                          <  2:97770/150‑1 (MQ=255)

      cAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAACCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTac                                                                                                                                          >  1:20214/1‑150 (MQ=255)

       aaaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTacc                                                                                                                                         <  1:674395/150‑1 (MQ=255)

        aaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTaccg                                                                                                                                        >  1:1303114/1‑150 (MQ=255)

        aaaaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACATaacg                                                                                                                                        >  1:953792/1‑150 (MQ=255)

             ccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGCAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACcgc                                                                                                                                   >  2:1791293/1‑150 (MQ=255)

             ccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACcgc                                                                                                                                   >  3:91811/1‑150 (MQ=255)

               aCGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGt                                                                                                                                 >  4:653466/1‑150 (MQ=255)

                 gCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGtga                                                                                                                               <  3:653466/150‑1 (MQ=255)

                    cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGngatga                                                                                                                            >  3:360420/1‑150 (MQ=255)

                        tCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAg                                                                                                                        >  3:695476/1‑150 (MQ=255)

                          gcgcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGcc                                                                                                                      <  3:433719/150‑1 (MQ=255)

                            gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGt                                                                                                                    <  4:459531/150‑1 (MQ=255)

                                 aGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                               <  1:1776078/150‑1 (MQ=255)

                                     ggTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGaa                                                                                                                           <  2:180797/134‑1 (MQ=255)

                                        gACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTc                                                                                                           >  3:609546/1‑147 (MQ=255)

                                        gACGTTGCAAACGCCATCCTAGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaac                                                                                                        <  2:413044/150‑1 (MQ=255)

                                            ttGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTac                                                                                                                                          <  1:1912436/112‑1 (MQ=255)

                                            ttGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTac                                                                                                                                          >  2:1912436/1‑112 (MQ=255)

                                            ttGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATg                                                                                                    >  2:191382/1‑150 (MQ=255)

                                             tGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGa                                                                                                   >  3:1209081/1‑150 (MQ=255)

                                                aaaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACaa                                                                                                >  2:1139760/1‑150 (MQ=255)

                                                    gCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGt                                                                                            <  2:416351/150‑1 (MQ=255)

                                                    gCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGt                                                                                            >  2:610728/1‑150 (MQ=255)

                                                      cATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaa                                                                                          >  4:468313/1‑150 (MQ=255)

                                                        tCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGGGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAAc                                                                                        >  2:976911/1‑150 (MQ=255)

                                                           tCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgc                                                                                     <  3:1237020/150‑1 (MQ=255)

                                                           tCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgc                                                                                     >  1:1759331/1‑150 (MQ=255)

                                                              aCGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCa                                                                                  <  1:1832571/150‑1 (MQ=255)

                                                                  tACTGACGCAGTGACGCTGTCTGGCGCATCCGAACAAGGTACCATCCCCCTCGCAGCGATTACAACCATGATGACTATATGAGAACGTACCGACCGCGTGATGACCAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                              <  3:638218/150‑1 (MQ=255)

                                                                   aCTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc                                                                             >  2:696852/1‑150 (MQ=255)

                                                                   aCTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTACTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc                                                                             >  1:1203016/1‑150 (MQ=255)

                                                                     tGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGa                                                                           <  4:226283/150‑1 (MQ=255)

                                                                     tGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGa                                                                           >  4:227667/1‑150 (MQ=255)

                                                                       aCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACACTGAACCACGCAAACTGCGCATTACCGAAg                                                                         <  2:944645/150‑1 (MQ=255)

                                                                        cGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGc                                                                        >  4:970980/1‑150 (MQ=255)

                                                                          cAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCgg                                                                      <  3:892970/150‑1 (MQ=255)

                                                                           aGTGATGCTGTCTGGCGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTCCTATCATGGCGACCATCTGAGAACGTACTGACCGCGTGATGAACAGCCGTCGCGAGCTCAACAATGACAACCGTAAACAGCGCAATACCGATGCgcg                                                                     >  4:321801/1‑148 (MQ=255)

                                                                             tGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTAt                                                                   <  2:359/150‑1 (MQ=255)

                                                                              gATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGcc                                                                                                                      >  2:1462944/1‑98 (MQ=255)

                                                                              gATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGcc                                                                                                                      <  1:1462944/98‑1 (MQ=255)

                                                                               aTGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGc                                                                 >  1:391824/1‑150 (MQ=255)

                                                                               aTGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGc                                                                 >  3:734325/1‑150 (MQ=255)

                                                                                 gCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCg                                                               <  1:1139760/150‑1 (MQ=255)

                                                                                 gCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCg                                                               >  1:2053099/1‑150 (MQ=255)

                                                                                   tGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                             <  3:131998/150‑1 (MQ=255)

                                                                                   tGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGGTTCTATCATGGCGACCATCTGAGAACGTACCTACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                             >  1:1033274/1‑150 (MQ=255)

                                                                                      cTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACcgc                                                                                                                                   <  2:1120909/77‑1 (MQ=255)

                                                                                         gTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCg                                                       >  2:500924/1‑150 (MQ=255)

                                                                                           gAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                                     >  3:641100/1‑150 (MQ=255)

                                                                                                 gCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAct                                               >  1:1796898/1‑150 (MQ=255)

                                                                                                    aaaGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgct                                            <  2:154179/150‑1 (MQ=255)

                                                                                                        gTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                                        >  4:820345/1‑150 (MQ=255)

                                                                                                         tAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                               <  3:884435/78‑1 (MQ=255)

                                                                                                         tAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                               >  4:884435/1‑78 (MQ=255)

                                                                                                         tAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaaa                                       <  4:52955/150‑1 (MQ=255)

                                                                                                         tAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaaa                                       <  2:1289932/150‑1 (MQ=255)

                                                                                                           aaTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                                     <  2:1172609/134‑1 (MQ=255)

                                                                                                           aaTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                                     >  1:1172609/1‑134 (MQ=255)

                                                                                                            aTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    <  3:215985/150‑1 (MQ=255)

                                                                                                            aTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    <  1:696852/150‑1 (MQ=255)

                                                                                                            aTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    <  4:641100/150‑1 (MQ=255)

                                                                                                            aTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    <  1:1326287/150‑1 (MQ=255)

                                                                                                            aTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAAAAAGGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                    <  3:666901/150‑1 (MQ=255)

                                                                                                             tgccggCTGGGAGCGGTTTCTATCATGGCGACCATCTGAGAACGTGCCGACCGCGTGAGGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                                   <  2:138644/145‑1 (MQ=255)

                                                                                                             tACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                                   <  3:161372/150‑1 (MQ=255)

                                                                                                             tACCCGCTGGAAGCGGGTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                                   <  2:20214/150‑1 (MQ=255)

                                                                                                               cccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                                 <  4:149962/150‑1 (MQ=255)

                                                                                                                ccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGAt                                >  2:62639/1‑150 (MQ=255)

                                                                                                                ccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCATAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGAt                                >  2:1067555/1‑150 (MQ=255)

                                                                                                                   cTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGaa                                                  >  1:104648/1‑129 (MQ=255)

                                                                                                                   cTGGAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGaa                                                  <  2:104648/129‑1 (MQ=255)

                                                                                                                      gAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCg                          <  1:564441/150‑1 (MQ=255)

                                                                                                                      gAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCg                          <  1:610728/150‑1 (MQ=255)

                                                                                                                      gAAGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCATAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCg                          <  1:1067555/150‑1 (MQ=255)

                                                                                                                       aaGCGGTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGc                         <  2:54952/150‑1 (MQ=255)

                                                                                                                           ggTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGAt                     <  3:491628/150‑1 (MQ=255)

                                                                                                                            gTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATc                    >  3:901152/1‑150 (MQ=255)

                                                                                                                            gTTTCTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCAGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATc                    >  4:721515/1‑150 (MQ=255)

                                                                                                                              ttCTATCATGGCGACCATCTGAGAACGTACCGACCGCGAGCTGAACAGCCGCCTCGAGTTCAACAAAGACAACCGTAACCTACGCATTACCGAAGCGGTATACCGTGGTGCCGTCGAAACTGCTGAACAAGTGGATGCTACGCTgcccgg                  >  2:1514390/1‑145 (MQ=255)

                                                                                                                                cTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg                <  4:639539/150‑1 (MQ=255)

                                                                                                                                cTATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg                <  4:734325/150‑1 (MQ=255)

                                                                                                                                 tATCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGt               >  4:981334/1‑150 (MQ=255)

                                                                                                                                  aTCATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGtt              <  4:1137394/150‑1 (MQ=255)

                                                                                                                                    cATGGCGACCATCTGGGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGc            <  1:976911/150‑1 (MQ=255)

                                                                                                                                    cATGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGc            <  3:1022277/150‑1 (MQ=255)

                                                                                                                                      tGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTa          >  4:881121/1‑150 (MQ=255)

                                                                                                                                      tGGCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTAAAAAACTGGATGCTCCGCTGATCGTGGTTGCTa          <  2:1759331/150‑1 (MQ=255)

                                                                                                                                        gCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaacaa                                                                                                      >  3:995474/1‑56 (MQ=255)

                                                                                                                                        gCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTAcc        >  4:1065600/1‑149 (MQ=255)

                                                                                                                                        gCGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACt        >  1:996298/1‑150 (MQ=255)

                                                                                                                                         cGACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTc       >  1:626272/1‑150 (MQ=255)

                                                                                                                                          gACCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCa      >  1:1409227/1‑150 (MQ=255)

                                                                                                                                           aCCATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAg     >  4:1148620/1‑150 (MQ=255)

                                                                                                                                             cATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAggg   >  3:1016732/1‑150 (MQ=255)

                                                                                                                                             cATCTGAGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAggg   <  3:227667/150‑1 (MQ=255)

CATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGC  >  NC_000913_3_pae_tpiA/1756546‑1756837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`