Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,315,8000AG21.1% 80.3 / 15.1 38intergenic (‑30/+56)yciF/yciGputative rubrerythrin/ferritin‑like metal‑binding protein/KGG family protein
Reads supporting (aligned to +/- strand):  ref base A (18/12);  new base G (4/4);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTTTCATTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCC  >  NC_000913_3_pae_tpiA/1315654‑1315948
                                                                                                                                                  |                                                                                                                                                    
tttttCATTTGATGTTGCTATTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATaa                                                                                                                                                   >  2:870058/1‑149 (MQ=255)
       tttGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGc                                                                                                                                            >  4:326195/1‑150 (MQ=255)
       tttGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGc                                                                                                                                            <  1:681982/150‑1 (MQ=255)
                    ttGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATc                                                                                                                               >  4:122396/1‑150 (MQ=255)
                           ttttGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTAAAAGCAGGTGAATAAACCCTTCTTCCGTGGTCTTCATATTCATATTTTTCTCCAGNGNAATCACNGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCa                                                                                                                        <  3:250217/150‑1 (MQ=255)
                                                  gTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTg                                                                                                 >  2:445771/1‑150 (MQ=255)
                                                   tttttCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCt                                                                                                                     >  2:362623/1‑129 (MQ=255)
                                                   tttttCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCt                                                                                                                     <  1:362623/129‑1 (MQ=255)
                                                       tCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCa                                                                                            <  3:544081/150‑1 (MQ=255)
                                                                        tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCGTATGGCTCGCa                                                                                                                                           >  4:179557/1‑86 (MQ=255)
                                                                        tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCGTATGGCTCGCa                                                                                                                                           <  3:179557/86‑1 (MQ=255)
                                                                        tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACg                                                                                                                                                  >  4:234737/1‑79 (MQ=255)
                                                                        tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACg                                                                                                                                                  <  3:234737/79‑1 (MQ=255)
                                                                            aGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCttt                                                                                                                            <  2:233394/97‑1 (MQ=255)
                                                                            aGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCttt                                                                                                                            >  1:233394/1‑97 (MQ=255)
                                                                               aGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTaccacc                                                                    <  1:870058/150‑1 (MQ=255)
                                                                               aGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTaccacc                                                                    >  2:548359/1‑150 (MQ=255)
                                                                                 gTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGt                                                                                                  >  2:268049/1‑118 (MQ=255)
                                                                                 gTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGt                                                                                                  <  1:268049/118‑1 (MQ=255)
                                                                                      taaataCATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTttgt                                                             >  3:367090/1‑150 (MQ=255)
                                                                                        aataCATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGCAgtga                                                                                                                                       <  4:185578/74‑4 (MQ=25)
                                                                                        aataCATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGCAgtga                                                                                                                                       >  3:185578/1‑71 (MQ=25)
                                                                                              tCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCCTTGTTGACCGcc                                                     >  4:520176/1‑150 (MQ=255)
                                                                                                ttCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAgg                                                                                          <  3:155736/111‑1 (MQ=255)
                                                                                                ttCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAgg                                                                                          >  4:155736/1‑111 (MQ=255)
                                                                                                          ttcatattcataTTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGc                                         >  1:461716/1‑150 (MQ=255)
                                                                                                             atattcatattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTc                                      <  2:461716/150‑1 (MQ=255)
                                                                                                             atattcatattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTc                                      <  1:286692/150‑1 (MQ=255)
                                                                                                                ttcatattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGa                                   >  3:297321/1‑150 (MQ=255)
                                                                                                                  catattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGc                                                                                    <  2:883622/99‑1 (MQ=255)
                                                                                                                  catattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGc                                                                                    >  1:883622/1‑99 (MQ=255)
                                                                                                                     attTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATgcgc                              >  3:537443/1‑150 (MQ=255)
                                                                                                                         ttCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTg                          <  4:367090/150‑1 (MQ=255)
                                                                                                                              cAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTAGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGAt                     >  4:355570/1‑150 (MQ=255)
                                                                                                                                         cTGCGGGCCGTATGGCTCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGa                      <  3:60966/138‑1 (MQ=255)
                                                                                                                                         cTGCGGGCCGTATGGCTCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGa                      >  4:60966/1‑138 (MQ=255)
                                                                                                                                          tGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt         >  4:226760/1‑150 (MQ=255)
                                                                                                                                                 cATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccgcc  >  2:464432/1‑150 (MQ=255)
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TTTTTCATTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCC  >  NC_000913_3_pae_tpiA/1315654‑1315948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑