New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1351239	44 (1.000)	6 (0.140)	6/272	5.0	13.6%	intergenic (‑200/+168)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
	?	NC_000913_3_pae_tpiA	3908518 =	34 (0.800)					intergenic (‑152/+31)	pstB/pstA	phosphate ABC transporter ATPase/phosphate ABC transporter permease
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACA  >  NC_000913_3_pae_tpiA/1351100‑1351375                                                                                                                                            |                                                                                                                                         gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                 <  2:601543/149‑1 (MQ=255) gTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                 <  1:603967/149‑1 (MQ=255)    aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTCGCCAGGACCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGGGGGGACCCGATtgtg                                                                                                                              >  1:731776/1‑149 (MQ=39)            gAAAAGATACACGGTTATTATAATCAACCTGGCTGTGAGTAGCTTTTGTTGCCAGGTCCGACCGGTGTAGTCTGCGGCAGGTGGGGCGCTTTTCCCTTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                      <  2:152748/149‑1 (MQ=37)              aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                                                                    <  2:388040/149‑1 (MQ=255)                 gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTGTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                                                                 <  1:150687/149‑1 (MQ=255)                    aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTATTAcccc                                                                                                              >  2:303662/1‑149 (MQ=255)                        ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGGGGGGACCGTATTGTGGAAATATTGTTACCCCAGca                                                                                                          >  1:118241/1‑149 (MQ=255)                        ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGGCTTGTGCAAATATTGTTAACCCAGca                                                                                                          >  2:306246/1‑149 (MQ=255)                         gTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa                                                                                                         >  2:684180/1‑149 (MQ=255)                                aaaTCAAGCTGGCTGTGAGGACCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg                                                                                                  <  2:34224/148‑1 (MQ=255)                                 aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGCGGGGGCCGTATTGTGCAAATATTGTTCCCCCCGCaccaaaccgt                                                                                                 >  1:62269/1‑146 (MQ=38)                                 aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                 <  2:328966/149‑1 (MQ=255)                                  aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGGCCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc                                                                                                >  1:520323/1‑149 (MQ=255)                                      aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAggtccta                                                                                            >  2:609997/1‑144 (MQ=255)                                       ccTGGATGTGAGTAGCTATAGTTGCCAGGTACGACAGGGACAATTTGGTGCACGTCTTGGGCTTGTCTGCGCAGCCGATCCCTCTCCCCATATGGTTTATGGTACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                                                                           <  2:652509/149‑1 (MQ=255)                                          ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAg                                                                                        <  1:540488/149‑1 (MQ=255)                                           gCTGTGATTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                       <  2:371649/149‑1 (MQ=255)                                             tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc                                                                                     <  1:253624/149‑1 (MQ=255)                                                 aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGTCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                 <  1:609997/149‑1 (MQ=255)                                                  gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCGCGCACCCGAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGATCATACAGCCCCTAAc                                                                                >  1:541646/1‑149 (MQ=255)                                                     gCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCTAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt                                                                             <  2:520323/149‑1 (MQ=255)                                                         taGTTGCCAGGTCCGCCCGGAGCAGGCTGCCGCAGGGCGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                         <  1:67484/149‑1 (MQ=255)                                                            ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                      >  2:101782/1‑149 (MQ=255)                                                                cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCCGCAAAAAGGGTCAGACAGCCCCTAACCCTTTCATGGCGac                                                                  >  1:605367/1‑148 (MQ=38)                                                                  ggTCCGGCCGGAGAAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCGAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATgg                                                                <  2:5362/149‑1 (MQ=255)                                                                       gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTTCGGTATGCCTAGAACTCGCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTGCCCCAGCCACAAACAGGCTCATACAGGCCCGAACCCTTTCATGGCGATGGCTggg                                                           >  1:578855/1‑149 (MQ=255)                                                                             aGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                     <  1:355345/149‑1 (MQ=255)                                                                                  gcggCGGCAGGGGGGGCGCTTTTCCCCGCGCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                <  2:731776/146‑1 (MQ=255)                                                                                                 gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtct                                 <  1:559869/149‑1 (MQ=255)                                                                                                       ttCCCTTCACCCTAACCCTCGCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCTTCACCCTTTCAGGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCa                           <  2:326232/149‑1 (MQ=39)                                                                                                         ccccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCATTTCATGGCGATGGCTGGGGCGCTTCAGAGCTTGCCGAGTATTTTCCGGCAca                         >  2:609350/1‑148 (MQ=38)                                                                                                           ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGGCCTTGCCGAATATTCTCCAGCACCgc                       >  2:358221/1‑148 (MQ=255)                                                                                                           ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                <  1:710480/124‑1 (MQ=255)                                                                                                           ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                >  2:710480/1‑124 (MQ=255)                                                                                                             tCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTCCCGAATATTCCCCAGCACCGtct                     >  2:253516/1‑149 (MQ=255)                                                                                                                    aaCCCTCCCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt              <  1:107860/149‑1 (MQ=255)                                                                                                                        ctcgCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca          <  1:684180/149‑1 (MQ=255)                                                                                                                            ccccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac      <  1:101782/149‑1 (MQ=255)                                                                                                                              ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaa    >  1:693572/1‑149 (MQ=255)                                                                                                                               cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                     <  1:693774/99‑1 (MQ=255)                                                                                                                               cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGtt                                                     >  2:693774/1‑99 (MQ=255)                                                                                                                                agagGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca  <  2:529036/149‑1 (MQ=255)                                                                                                                                agagGGGCGAGGGGACCGTATTGTGCAAACATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCTTGGCGATGGCTGGGACGGCTCAGGCCTTGCCGAATGTTCTCCAGCACCGTCTCCATGTTTTCACCacaaca  <  2:529143/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         GTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACA  >  NC_000913_3_pae_tpiA/1351100‑1351375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.