Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	43 [0]	[0] 43	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	40 (0.960)	38/266	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_pae_tpiA/1978357‑1978644                                                                                                                                                   |                                                                                                                                               aaaTGGGTCTTTACACTTATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                             <  1:599106‑M1/149‑4 (MQ=255)   aTGGGTCTTTACACTTATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                           <  1:303383‑M1/149‑6 (MQ=255)    tGGGTCTTTACACTTATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATACCACAAAACACTCAATTACTTACCCTACATGtttacg                                                                                                                                          >  2:336738‑M1/1‑143 (MQ=255)       gTCTTTACACTTATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                       >  1:127524‑M1/1‑140 (MQ=255)                gctttgtaagattTTTTCCAAACTCGAGGCAACTGTATTAGGCACTACAAGCATATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATTCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                                                               <  2:298849‑M1/143‑18 (MQ=255)                 ttATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                             >  1:427483‑M1/1‑130 (MQ=255)                 ttATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAAAACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                             >  2:194822‑M1/1‑130 (MQ=255)                  tATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt                                                                                                                            <  1:638196‑M1/149‑21 (MQ=255)                     cTAACA‑TGTTTCCGAACTCGACGCAACTGTAATAATCCCTCCACCCACGTACAACTGAGGGGGGTTGCGATTTTCAATAATGTGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                         <  2:43844‑M1/149‑24 (MQ=255)                       aaGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                                                                       <  1:473682‑M1/149‑26 (MQ=255)                          a‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCAGCGATTTTCAATAATGCGTCATGCAGATCACACAAAACACTCAATTACTTAACATACATGtataagtcatacttttgttttcggtgta                                                                                                                    >  2:377122‑M1/1‑121 (MQ=255)                                  cTAAATCGACGCAACTGTACTCGTCACTACATGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACCCTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                             <  2:493464‑M1/149‑36 (MQ=255)                                     aaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTAAACATAAATGtataagtaatacttttgttttaggtgtatttcaatctg                                                                                                          >  2:317013‑M1/1‑111 (MQ=255)                                          aCGCAACTGTACTCGGCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                                                                     >  1:412630‑M1/1‑106 (MQ=255)                                              aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                                                                 >  1:649638‑M1/1‑102 (MQ=255)                                                  ttaCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                             <  2:12342‑M1/148‑52 (MQ=255)                                                      tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGAAAACACAAAACACTCAATTACTTAACATAAATTtataaggcatacttttgttttgggtgtttttcaatatgttaaacagtttttctct                                                                                         >  1:219435‑M1/1‑94 (MQ=255)                                                        gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtcaaaacatttttcgctac                                                                                       >  2:149428‑M1/1‑92 (MQ=255)                                                            cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                   >  1:375772‑M1/1‑88 (MQ=255)                                                                 cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                                                              <  2:427483‑M1/149‑67 (MQ=255)                                                                      aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttgaaaagtttttcgctacgctagcaagcaaaa                                                                         >  1:117174‑M1/1‑78 (MQ=255)                                                                       taaaacggAGGGTGGCTGCTATTTTCAATAATGCGTGATGAAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                        <  2:262408‑M1/146‑73 (MQ=255)                                                                          aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtatacgtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                     >  1:716910‑M1/1‑74 (MQ=255)                                                                            cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAAATAAATGtataagtcagacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagctagcaaaaatgaaa                                                                   >  2:588871‑M1/1‑72 (MQ=255)                                                                                 ggggtCTTCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                              <  2:35400‑M1/149‑83 (MQ=255)                                                                                 gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttccatctgttaaaaagtttttcgctacgctaccacgcaaacatgcaacagga                                                              >  2:343632‑M1/1‑67 (MQ=255)                                                                                          gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaa                                                     >  1:136540‑M1/1‑58 (MQ=255)                                                                                                 aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatagaaatgggat                                              >  1:531380‑M1/1‑51 (MQ=255)                                                                                                     atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttgaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttg                                          <  1:149100‑M1/149‑103 (MQ=255)                                                                                                       gCGGGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                        <  2:2339‑M1/149‑105 (MQ=255)                                                                                                         gTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                   <  1:228320‑M1/104‑62 (MQ=255)                                                                                                         gTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                                                                   >  2:228320‑M1/1‑43 (MQ=255)                                                                                                           gATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                    <  1:194822‑M1/149‑109 (MQ=255)                                                                                                                   tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgaacagtcaaaat                            >  1:177127‑M1/1‑33 (MQ=255)                                                                                                                     acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                          <  1:631583‑M1/149‑119 (MQ=255)                                                                                                                               cTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaa                >  1:618154‑M1/1‑21 (MQ=255)                                                                                                                                tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa               >  1:199646‑M1/1‑20 (MQ=255)                                                                                                                                   aTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacaggcaaaataactcaccgtaaataa            >  1:317999‑M1/1‑17 (MQ=255)                                                                                                                                          aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctg     >  1:596584‑M1/1‑10 (MQ=255)                                                                                                                                           aCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc    <  1:149428‑M1/149‑141 (MQ=255)                                                                                                                                            cATAAATGtataagtcatactattgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgct   >  2:686493‑M1/1‑8 (MQ=255)                                                                                                                                             aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  <  1:80754‑M1/149‑143 (MQ=255)                                                                                                                                             aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  >  2:744563‑M1/1‑7 (MQ=255)                                                                                                                                                   |                                                                                                                                               AAATGGGTCTTTACACTTATCTAAGA‑TTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_pae_tpiA/1978357‑1978644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑