New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 161327590 (0.630)18 (0.130) 16/248 13.6 17.8% intergenic (‑24/+40) glsB/sad glutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
?NC_000913_3_pae_tpiA = 1613279 82 (0.610)intergenic (‑28/+36) glsB/sad glutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1613121‑1613275
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcaggtctTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGGTTCCCGAT  <  NC_000913_3_pae_tpiA/1613279‑1613131
                                                                                                                                                                                                                                                                                                                
GCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCA                                                                                                                                                            >  2:1271836/1‑150
 CAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAG                                                                                                                                                           >  2:1087406/1‑150
     CCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCT                                                                                                                                                       >  3:437707/1‑150
                AACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTG                                                                                                                                            <  1:639996/150‑1
                    GTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGG                                                                                                                                         <  1:1167377/149‑1
                    GTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGG                                                                                                                                         >  2:1167377/1‑149
                                 AGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAA                                                                                                                           <  4:790781/150‑1
                                         AATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGT                                                                                                                   <  4:849132/150‑1
                                                             CCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGA                                                                                                                             <  1:995748/120‑1
                                                             CCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGA                                                                                                                             >  2:995748/1‑120
                                                                    AATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGT                                                                                                                                                         >  1:1461750/1‑85
                                                                    AATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGT                                                                                                                                                         <  2:1461750/85‑1
                                                                       GAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGA                                                                                                                                  <  3:190054/105‑1
                                                                       GAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGA                                                                                                                                  >  4:190054/1‑105
                                                                                        GCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTG                                                                                                                                            >  3:618873/1‑78
                                                                                        GCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTG                                                                                                                                            <  4:618873/78‑1
                                                                                                              ATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTG                                                                                                                                            >  3:173271/1‑56
                                                                                                              ATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTG                                                                                                                                            <  4:173271/56‑1
                                                                                                                   CCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCA                                                                                                                                     <  2:563148/58‑1
                                                                                                                   CCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCA                                                                                                                                     >  1:563148/1‑58
                                                                                                                     ATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAG                                                                                                                                 <  1:386771/60‑1
                                                                                                                     ATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAG                                                                                                                                 >  2:386771/1‑60
                                                                                                                                                ACCGCAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGAC            >  3:955044/1‑150
                                                                                                                                                  CGCAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGG          <  2:1099751/150‑1
                                                                                                                                                    CAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTA                                     >  3:744901/1‑121
                                                                                                                                                    CAGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTA                                     <  4:744901/121‑1
                                                                                                                                                     AGGTCTTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGGTTC       >  4:420182/1‑150
                                                                                                                                                          TTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGGTTCCCGAT  <  4:685851/150‑1
                                                                                                                                                          TTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGGTTCCCGAT  >  3:475372/1‑150
                                                                                                                                                                                                                                                                                                                
GCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1613121‑1613275
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcaggtctTTTCAGACCTGCGGTCCAGGAGTAGAAAGTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGGTTCCCGAT  <  NC_000913_3_pae_tpiA/1613279‑1613131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.