New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 183652 =132 (0.920)14 (0.110) 14/246 14.5 10.7% intergenic (+32/+57) cdaR/yaeH carbohydrate diacid regulon transcriptional regulator; autoregulator/UPF0325 family protein
?NC_000913_3_pae_tpiA 183685 = 112 (0.850)intergenic (+65/+24) cdaR/yaeH carbohydrate diacid regulon transcriptional regulator; autoregulator/UPF0325 family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTAAGCGTAAGATCCTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/183810‑183652
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaggggaatgCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCT  >  NC_000913_3_pae_tpiA/183685‑183833
                                                                                                                                                                                                                                                                                                                    
CTTAAGCGTAAGATCCTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTG                                                                                                                                                                >  4:356534/1‑150
   AAGCGTAAGATCCTCGACGACTTACTTCACCTGGAGTCAGTCGTATCCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAATCTAACGCGTAAATAATCCCGAACTGACGGTTATTTCGGGCATTCCCCTCTACCCTTCGGGCTGAGG                                                                                                                                                             >  1:505938/1‑150
   AAGCGTAAGATCCTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGG                                                                                                                                                             <  4:17821/150‑1
   AAGCGTAAGATCCTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGG                                                                                                                                                             <  1:650984/150‑1
    AGCGTAAGATCCTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGCTCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGG                                                                                                                                                            <  2:1025304/150‑1
        TAAGATCCTCGACGACGTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAAT                                                                                                                                                        >  2:859123/1‑150
               CTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCACGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGG                                                                                                                                                            >  4:560600/1‑139
               CTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCACGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGG                                                                                                                                                            <  3:560600/139‑1
                              CACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGA                                                                                                                                                          >  1:971373/1‑126
                              CACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGA                                                                                                                                                          <  2:971373/126‑1
                                                              CGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGA                                                                                                                                                               >  1:628461/1‑89
                                                              CGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGA                                                                                                                                                               <  2:628461/89‑1
                                                                                                         CGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATC                                                                                 >  2:1637161/1‑124
                                                                                                         CGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATC                                                                                 <  1:1637161/124‑1
                                                                                                                        TTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAG                                                <  4:237658/142‑1
                                                                                                                        TTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAG                                                >  3:237658/1‑142
                                                                                                                             GCATTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGA                                                                                                      <  1:764582/83‑1
                                                                                                                             GCATTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGA                                                                                                      >  2:764582/1‑83
                                                                                                                                TTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATT                                                                            <  1:151138/106‑1
                                                                                                                                TTCCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATT                                                                            >  2:151138/1‑106
                                                                                                                                  CCCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCCGTTCGGGATTATTTACGCGGTAGTGTTTCCAGATCGGCTTCAATCTCGCTGAGCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGC                              >  4:713398/1‑150
                                                                                                                                   CCCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCT                             >  3:49238/1‑150
                                                                                                                                    CCTCTACCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTT                            >  3:493740/1‑150
                                                                                                                                          CCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCC                                                                                                         <  2:188753/67‑1
                                                                                                                                          CCCTTCGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCC                                                                                                         >  1:188753/1‑67
                                                                                                                                               CGGGCTGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTT                 <  4:493740/150‑1
                                                                                                                                                     GAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACT                                                                  >  3:805781/1‑95
                                                                                                                                                     GAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACT                                                                  <  4:805781/95‑1
                                                                                                                                                      AGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCG          <  2:123195/150‑1
                                                                                                                                                       GGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGG         <  4:826407/150‑1
                                                                                                                                                          GAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCA      <  1:1025304/150‑1
                                                                                                                                                              GCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCT  <  3:102301/150‑1
                                                                                                                                                                                                                                                                                                                    
CTTAAGCGTAAGATCCTCGACGACTTACGTCACCTGGAGTCAGTCGTAACCAATAAGATCAGCGAGATTGAAGCCGATCTGGAAAAACTAACGCGTAAATAATCCCGAACTGACGGTTAATTCGGGCATTCCCCTCTACCCTTCGGGCTGAGGGGAATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/183810‑183652
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gaggggaatgCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTACGCTTAAGATCAACTTCGCTGCGGTCACGCT  >  NC_000913_3_pae_tpiA/183685‑183833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.