Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,352,6590TG28.9% 194.3 / 132.5 137S54A (TCC→GCC) ghoSantitoxin of GhoTS toxin‑antitoxin pair; endonuclease for ghoT mRNA
Reads supporting (aligned to +/- strand):  ref base T (50/46);  new base G (23/16);  total (73/64)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 137 total aligned reads displayed.

AACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGG  >  NC_000913_3_pae_tpiA/4352512‑4352805
                                                                                                                                                   |                                                                                                                                                  
aaCAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTcc                                                                                                                                                  <  1:53190/150‑1 (MQ=255)
   aaGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTc                                                                                                                                               <  4:945375/150‑1 (MQ=255)
   aaGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTc                                                                                                                                               <  1:1409390/150‑1 (MQ=255)
   aaGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTc                                                                                                                                               <  2:79386/150‑1 (MQ=255)
   aaGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTc                                                                                                                                               >  3:581457/1‑150 (MQ=255)
    aGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCg                                                                                                                                              <  3:680841/150‑1 (MQ=255)
    aGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCg                                                                                                                                              <  2:694640/150‑1 (MQ=255)
         aaTACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATa                                                                                                                                         >  1:31893/1‑150 (MQ=255)
          aTACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATaa                                                                                                                                        <  1:1609708/150‑1 (MQ=255)
           tACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCaa                                                                                                                                           <  1:1331815/146‑1 (MQ=255)
           tACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCaa                                                                                                                                           >  2:1331815/1‑146 (MQ=255)
             cTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGa                                                                                                                                     <  1:412785/150‑1 (MQ=255)
                   ttgttTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGt                                                                                                                               >  4:312070/1‑150 (MQ=255)
                   ttgttTCTTTTGATTATCCATCATCTTATTCCTAAGTGTTCTTAAGATTAAGATCATTGATGTATGTTATGAATTTCTCCTCTATAGTGTCTGATGAATATGGGATACCACGCCAATTGAATGCACACTCCTTCTCAATACCGACATCGt                                                                                                                               >  2:1482340/1‑150 (MQ=255)
                    tgttTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGtt                                                                                                                              <  2:517392/150‑1 (MQ=255)
                     gttTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTa                                                                                                                             <  4:158457/150‑1 (MQ=255)
                        tCTTTTGATCATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGcc                                                                                                                          >  1:1119877/1‑150 (MQ=255)
                          ttttGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGc                                                                                                                        >  4:87903/1‑150 (MQ=255)
                            ttGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCaa                                                                                                                      <  3:356039/150‑1 (MQ=255)
                            ttGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCaa                                                                                                                      >  1:621506/1‑150 (MQ=255)
                                     catcatCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTACCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCg                                                                                                             >  4:852066/1‑150 (MQ=255)
                                      atcatcTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGa                                                                                                            >  3:276027/1‑150 (MQ=255)
                                      atcatcTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGa                                                                                                            >  1:939306/1‑150 (MQ=255)
                                       tcatcTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGaa                                                                                                           <  2:621506/150‑1 (MQ=255)
                                        catcTTAGTCCTCAGTATTCTTAAGTTTAAGCTCATTGATGTGTGATATGAATTTCTCCTCTATCGTGGCTGATGAATATTGTATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAg                                                                                                          >  4:864520/1‑150 (MQ=255)
                                          tcTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGAt                                                                                                        >  1:1595011/1‑150 (MQ=255)
                                           cTTATGCATCAGTGTTTTGTAGATTGAGATGGTTGATGTATGGTATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGCTGCCACGACAATAGAATGACAACTCCTTCGAAATAACGACATCGTTAGCCGCAAGTGAAATCGAAAAtt                                                                                                       <  4:167837/150‑1 (MQ=255)
                                              aTTCCTCAGTGTTCTTAAGATTCAGATTATTGATGTATCATATGAACTTGTCCTCTATCGTGGCTGTTGAGTATGGGATACCACGACAGTTGAATGAAAACACCTTGGCTATAACGACAGCGTTAGCCGCGAGTGGTATCGAAGTTTTaa                                                                                                    <  4:244305/150‑1 (MQ=255)
                                              aTTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTaa                                                                                                    <  4:142187/150‑1 (MQ=255)
                                                  cTCAGTGTTCTTAAGATTAAGATCTTTGACGTATGATATGAATTTCTCCTCTATCGTGGCCGATGAATATGGGATACAACCACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTCAGCCGCAAGTGAAATCGAAGATTTAATCAg                                                                                                >  2:1046344/1‑150 (MQ=255)
                                                  cTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCg                                                                                                                                >  1:970488/1‑118 (MQ=255)
                                                  cTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCg                                                                                                                                <  2:970488/118‑1 (MQ=255)
                                                  cTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAg                                                                                                >  3:463317/1‑150 (MQ=255)
                                                   tCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCaa                                                                                                                      <  2:59520/127‑1 (MQ=255)
                                                   tCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGCTTTAATCAgg                                                                                               >  1:832995/1‑150 (MQ=255)
                                                    cAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTc                                                                                                                                               >  3:149293/1‑101 (MQ=255)
                                                      gtgtTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTc                                                                                            <  1:1034262/150‑1 (MQ=255)
                                                           cTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATg                                                                                       >  3:876468/1‑150 (MQ=255)
                                                              aagattaagatCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCtt                                                                                    >  1:643325/1‑150 (MQ=255)
                                                               agattaagatCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTa                                                                                   <  2:1361548/150‑1 (MQ=255)
                                                                    aagatCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACtt                                                                              >  3:611075/1‑150 (MQ=255)
                                                                      gatCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTaa                                                                                                    >  2:1637876/1‑126 (MQ=255)
                                                                      gatCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTAc                                                                            >  2:994695/1‑150 (MQ=255)
                                                                       atCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTAcc                                                                           <  3:312070/150‑1 (MQ=255)
                                                                        tCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATATAATCAGGCTCAAATGCTTAGACTTACCg                                                                          >  2:1482072/1‑150 (MQ=255)
                                                                        tCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCg                                                                          >  3:554535/1‑150 (MQ=255)
                                                                           ttGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGat                                                                       >  4:528546/1‑150 (MQ=255)
                                                                                     atatgaATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTg                                                             >  2:947892/1‑150 (MQ=255)
                                                                                      tatgaATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGa                                                            <  1:871062/150‑1 (MQ=255)
                                                                                       atgaATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGAc                                                           >  3:716241/1‑150 (MQ=255)
                                                                                         gaATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTg                                                             >  1:318709/1‑146 (MQ=255)
                                                                                         gaATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTg                                                             <  2:318709/146‑1 (MQ=255)
                                                                                                tcctcTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACAtt                                                  <  2:1266595/150‑1 (MQ=255)
                                                                                                 cctcTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCa                                                                                                 <  4:839964/102‑1 (MQ=255)
                                                                                                 cctcTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCa                                                                                                 >  3:839964/1‑102 (MQ=255)
                                                                                                  ctctATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCGATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGa                                                                                 <  1:273725/117‑1 (MQ=255)
                                                                                                  ctctATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCGATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGa                                                                                 >  2:273725/1‑117 (MQ=255)
                                                                                                  ctctATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAAc                                                                                                                                       <  4:492757/63‑1 (MQ=255)
                                                                                                    ctATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGt                                                                                                                               >  1:734071/1‑69 (MQ=255)
                                                                                                    ctATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGt                                                                                                                               <  2:734071/69‑1 (MQ=255)
                                                                                                          tGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtg                                        >  4:620429/1‑150 (MQ=255)
                                                                                                           ggCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtga                                       <  4:357585/150‑1 (MQ=255)
                                                                                                           ggCTGATGAATATGGGATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtga                                       <  2:1595011/150‑1 (MQ=255)
                                                                                                            gCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtgat                                      <  1:1580745/150‑1 (MQ=255)
                                                                                                            gCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtgat                                      <  1:1509849/150‑1 (MQ=255)
                                                                                                             cTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtgatg                                     >  1:6383/1‑150 (MQ=255)
                                                                                                              tgatgaATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTaa                                                                                                    <  1:135975/86‑1 (MQ=255)
                                                                                                              tgatgaATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAg                                                                                                >  4:364886/1‑90 (MQ=255)
                                                                                                              tgatgaATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAg                                                                                                <  3:364886/90‑1 (MQ=255)
                                                                                                                    atatGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGAcagacg                              >  1:116266/1‑145 (MQ=255)
                                                                                                                    atatGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTAttt                              >  1:1128939/1‑150 (MQ=255)
                                                                                                                    atatGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTAttt                              <  2:846146/150‑1 (MQ=255)
                                                                                                                      atGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTcc                            <  3:852066/150‑1 (MQ=255)
                                                                                                                        gggATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCaa                                                      <  2:282184/122‑1 (MQ=255)
                                                                                                                        gggATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCaa                                                      >  1:282184/1‑122 (MQ=255)
                                                                                                                        gggATACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGt                          <  4:6130/150‑1 (MQ=255)
                                                                                                                         ggATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTc                         >  3:476423/1‑150 (MQ=255)
                                                                                                                         ggATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTc                         >  2:1454209/1‑150 (MQ=255)
                                                                                                                          gATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCa                        <  2:643325/150‑1 (MQ=255)
                                                                                                                            tACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAg                                                                                                                            >  1:443626/1‑48 (MQ=255)
                                                                                                                            tACCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAg                                                                                                                            <  2:443626/48‑1 (MQ=255)
                                                                                                                             aCCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTg                                                                   <  3:737098/104‑1 (MQ=255)
                                                                                                                             aCCACGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTg                                                                   >  4:737098/1‑104 (MQ=255)
                                                                                                                                aCGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTa                  >  3:594208/1‑150 (MQ=255)
                                                                                                                                aCGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTa                  >  2:1213261/1‑150 (MQ=255)
                                                                                                                                aCGACAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTa                  >  3:513876/1‑150 (MQ=255)
                                                                                                                                   aCAATTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACaa               <  1:747238/150‑1 (MQ=255)
                                                                                                                                     aaTTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGAtttt                                                              <  2:967146/101‑1 (MQ=255)
                                                                                                                                     aaTTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGAtttt                                                              >  1:967146/1‑101 (MQ=255)
                                                                                                                                     aaTTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtgat                                      >  1:1487577/1‑125 (MQ=255)
                                                                                                                                     aaTTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTtgat                                      <  2:1487577/125‑1 (MQ=255)
                                                                                                                                     aaTTGAATGAAAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGt             <  3:150133/150‑1 (MQ=255)
                                                                                                                                       ttGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGATAGTTGATGACTATTTCCGTCAGTTTTACAAGTAg           >  1:1205252/1‑150 (MQ=255)
                                                                                                                                       ttGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAg           >  1:994721/1‑150 (MQ=255)
                                                                                                                                          aatgaaAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACaa               >  3:945400/1‑143 (MQ=255)
                                                                                                                                            tgaaAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGATCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg      >  1:1204630/1‑150 (MQ=255)
                                                                                                                                            tgaaAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg      >  2:1400895/1‑150 (MQ=255)
                                                                                                                                             gaaAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGATGATTTAATCAGGCTCAAATGCTTGGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga     <  2:1119877/150‑1 (MQ=255)
                                                                                                                                             gaaAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga     >  2:41695/1‑150 (MQ=255)
                                                                                                                                             gaaAACGCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga     >  1:231486/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATTAAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACTCCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGG  >  NC_000913_3_pae_tpiA/4352512‑4352805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: