New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 2700550 =50 (0.860)11 (0.220) 10/240 5.0 20.7% noncoding (31/77 nt) ohsC sRNA antisense regulator of shoB toxin
?NC_000913_3_pae_tpiA 2700601 = 41 (0.820)intergenic (+5/+17) ohsC/acpS sRNA antisense regulator of shoB toxin/holo‑[acyl‑carrier‑protein] synthase 1
Rejected: Coverage evenness skew score above cutoff.

CACGTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTT  >  NC_000913_3_pae_tpiA/2700411‑2700698
                                                                                                                                           |                                                                                                                                                    
cACGTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaa                                                                                                                                            <  4:269547/150‑1 (MQ=255)
    ttAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaaa                                                                                                                                           >  2:615010/1‑147 (MQ=255)
    ttAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTaaa                                                                                                                                           <  1:615010/147‑1 (MQ=255)
    ttAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTGTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAcc                                                                                                                                        <  3:56141/150‑1 (MQ=255)
        tCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTCGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCg                                                                                                                                    <  1:310497/150‑1 (MQ=255)
            tCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTcc                                                                                                                                <  3:147847/150‑1 (MQ=255)
               aTCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGGAAAAGGGAAGTACACTGATTCATGATCGTTGTTTTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTa                                                                                                                             >  1:216920/1‑150 (MQ=255)
                tCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTAc                                                                                                                            <  2:552671/150‑1 (MQ=255)
                    tatTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGt                                                                                                                        >  2:534746/1‑150 (MQ=255)
                    tatTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGt                                                                                                                        >  2:239389/1‑150 (MQ=255)
                         cTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCggg                                                                                                                   <  1:107290/150‑1 (MQ=255)
                          tttCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCgggg                                                                                                                  >  1:321209/1‑150 (MQ=255)
                            tcttcGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGt                                                                                                                >  3:79967/1‑150 (MQ=255)
                                     aGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACtttt                                                                                                       <  3:150725/150‑1 (MQ=255)
                                       gCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTa                                                                                                     <  4:368566/150‑1 (MQ=255)
                                       gCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTa                                                                                                     <  4:368563/150‑1 (MQ=255)
                                         tCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                                                                   <  4:193222/150‑1 (MQ=255)
                                         tCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                                                                   <  4:31831/150‑1 (MQ=255)
                                             gTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAgaga                                                                                               >  1:432434/1‑150 (MQ=255)
                                              tttttATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGaa                                                                                              >  4:48637/1‑150 (MQ=255)
                                               ttttATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAAc                                                                                             >  2:143613/1‑150 (MQ=255)
                                                  tATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGt                                                                                          <  3:205451/150‑1 (MQ=255)
                                                   aTGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGta                                                                                         <  1:335026/150‑1 (MQ=255)
                                                         aaGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTAttt                                                                                   >  1:30939/1‑150 (MQ=255)
                                                                cAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACtt                                                                            >  4:387620/1‑150 (MQ=255)
                                                                    ttGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAccccc                                                                                                                                     >  2:598069/1‑89 (MQ=255)
                                                                    ttGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAAccccc                                                                                                                                     <  1:598069/89‑1 (MQ=255)
                                                                              aaGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGt                                                              >  2:68975/1‑150 (MQ=255)
                                                                                ggtAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTAATTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTgg                                                            <  2:216920/148‑1 (MQ=255)
                                                                                  tAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                                                                   <  1:575867/109‑1 (MQ=255)
                                                                                  tAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTaaa                                                                                                   >  2:575867/1‑109 (MQ=255)
                                                                                             aTCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTg                                               >  2:36094/1‑150 (MQ=255)
                                                                                                 aTAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGc                                           <  1:385432/150‑1 (MQ=255)
                                                                                                    aTCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCa                                        >  2:70914/1‑150 (MQ=255)
                                                                                                           ttgttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCa                                 >  1:441710/1‑150 (MQ=255)
                                                                                                           ttgttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCACGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTTGCACAAGCATAGTGCCGCTCATCTGCCa                                 >  1:143471/1‑150 (MQ=255)
                                                                                                            tgttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAg                                <  3:54400/150‑1 (MQ=255)
                                                                                                              ttgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCg                              <  4:266880/150‑1 (MQ=255)
                                                                                                                gAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGtt                            <  2:30939/150‑1 (MQ=255)
                                                                                                                 aGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa                           <  1:385766/150‑1 (MQ=255)
                                                                                                                   ggTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCATCGTTACa                         >  2:255797/1‑150 (MQ=255)
                                                                                                                       cATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCa                     >  4:297139/1‑150 (MQ=255)
                                                                                                                           ctgcACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATAtt                 >  3:223839/1‑150 (MQ=255)
                                                                                                                             gcACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTg               <  2:441710/150‑1 (MQ=255)
                                                                                                                                caAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCaa            <  4:79967/150‑1 (MQ=255)
                                                                                                                                 aaaaTTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAAc           >  2:69920/1‑150 (MQ=255)
                                                                                                                                  aaaTTAAAGTTAAAAAGTAACACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACg          <  3:216445/150‑1 (MQ=255)
                                                                                                                                     tttaaGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAAGGccc       >  4:345306/4‑150 (MQ=255)
                                                                                                                                      tAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCa      <  2:146675/150‑1 (MQ=255)
                                                                                                                                          gTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCtt  >  2:54373/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
CACGTTAATCTTTCCATCAATATTGCTTTCTTCGTAAAGGCTCGAGTTTTTATGCTAAAGATTGCAAGTTGCTTGTAAAAGATAAGTACACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTT  >  NC_000913_3_pae_tpiA/2700411‑2700698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.