Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,159,112 G→A G236E (GGG→GAG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,159,1120GA100.0% 367.5 / NA 105G236E (GGG→GAG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/56);  total (49/56)

Only 100 of 106 total aligned reads displayed.

CAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAT  >  NC_000913_3_pae_tpiA/4158965‑4159251
                                                                                                                                                   |                                                                                                                                           
cAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCgaga                                                                                                                                           <  3:141938/150‑1 (MQ=255)
 aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGAt                                                                                                                                          <  3:256106/150‑1 (MQ=255)
 aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGAt                                                                                                                                          <  3:2353/150‑1 (MQ=255)
  ggggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATc                                                                                                                                         >  2:516481/1‑150 (MQ=255)
  ggggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATc                                                                                                                                         >  3:270603/1‑150 (MQ=255)
   gggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCa                                                                                                                                        <  1:412801/150‑1 (MQ=255)
   gggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTCGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCa                                                                                                                                        >  4:183908/1‑150 (MQ=255)
       aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACttt                                                                                                                                    <  2:99580/150‑1 (MQ=255)
        aaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTa                                                                                                                                   <  4:259315/150‑1 (MQ=255)
          aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACt                                                                                                                                 <  1:571163/150‑1 (MQ=255)
          aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACt                                                                                                                                 >  2:205353/1‑150 (MQ=255)
          aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACt                                                                                                                                 <  2:350736/150‑1 (MQ=255)
          aCTGCTGATGATGGCAGATGGTCAATGTTTGTGTGATCAGGAAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACCCTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACt                                                                                                                                 <  2:34088/150‑1 (MQ=255)
            tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGc                                                                                                                               <  2:573181/150‑1 (MQ=255)
             gctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGcc                                                                                                                              <  1:374733/150‑1 (MQ=255)
              ctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCa                                                                                                                             >  1:522299/1‑150 (MQ=255)
                gATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAgc                                                                                                                           >  2:340044/1‑150 (MQ=255)
                 aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAgcg                                                                                                                          >  1:392402/1‑150 (MQ=255)
                     tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCgctgg                                                                                                                      >  4:179385/1‑150 (MQ=255)
                      ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCgctggc                                                                                                                     <  3:183992/150‑1 (MQ=255)
                            tGGTCGCTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTgcc                                                                                                               <  4:223833/150‑1 (MQ=255)
                              gTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTgccgc                                                                                                             >  3:319822/1‑150 (MQ=255)
                               tCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTgccgcc                                                                                                            >  3:208920/1‑150 (MQ=255)
                                    gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                                                       <  2:299483/150‑1 (MQ=255)
                                    gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                                                       <  4:113106/150‑1 (MQ=255)
                                    gTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                                                       <  3:276724/150‑1 (MQ=255)
                                    gTTTGCGCGATCAGGCAATAGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATTGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgc                                                                                                       <  1:366960/150‑1 (MQ=255)
                                     tttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGGGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                                                                                      >  4:346378/1‑150 (MQ=255)
                                     tttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                                                                                      >  4:273268/1‑150 (MQ=255)
                                          gcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                                                                                 <  3:358231/150‑1 (MQ=255)
                                          gcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                                                                                 >  1:242008/1‑150 (MQ=255)
                                             aTCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgcg                                                                                              >  2:597971/1‑150 (MQ=255)
                                                  gCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGAt                                                                                                                                          >  1:221898/1‑101 (MQ=255)
                                                  gCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGAt                                                                                                                                          <  2:221898/101‑1 (MQ=255)
                                                  gCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATggg                                                                                         >  1:310049/1‑150 (MQ=255)
                                                    aaTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgggg                                                                                        <  4:277519/149‑1 (MQ=255)
                                                    aaTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgggg                                                                                        >  3:277519/1‑149 (MQ=255)
                                                    aaTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgt                                                                                       >  1:406563/1‑150 (MQ=255)
                                                    aaTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgt                                                                                       <  1:597971/150‑1 (MQ=255)
                                                     aTGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgtt                                                                                      >  1:272900/1‑150 (MQ=255)
                                                        ggTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgtt                                                                                   <  1:540766/150‑1 (MQ=255)
                                                         gTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGGCGGAGCGCAAACGCGATGGGGTTGttt                                                                                  <  1:449882/150‑1 (MQ=255)
                                                         gTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGCTGttt                                                                                  <  4:319822/150‑1 (MQ=255)
                                                           ttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                                >  1:488804/1‑150 (MQ=255)
                                                              tGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTg                                                                             <  4:120032/150‑1 (MQ=255)
                                                              tGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTg                                                                             >  3:385530/1‑150 (MQ=255)
                                                              tGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTg                                                                             >  1:7324/1‑150 (MQ=255)
                                                                  ttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAGCTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGt                                                                         <  4:45733/150‑1 (MQ=255)
                                                                   tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                                                                                  >  2:580479/1‑124 (MQ=255)
                                                                   tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                                                                                  <  1:580479/124‑1 (MQ=255)
                                                                   tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTg                                                                        <  1:570029/150‑1 (MQ=255)
                                                                    gAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc                                                                       >  1:36576/1‑150 (MQ=255)
                                                                     aaGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCa                                                                      <  1:564176/150‑1 (MQ=255)
                                                                       gCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGt                                                                         >  2:79343/1‑145 (MQ=255)
                                                                       gCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAtt                                                                    <  4:152627/150‑1 (MQ=255)
                                                                       gCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAtt                                                                    <  2:310049/150‑1 (MQ=255)
                                                                       gCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAtt                                                                    <  3:183908/150‑1 (MQ=255)
                                                                       gCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACAGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAtt                                                                    >  2:475339/1‑150 (MQ=255)
                                                                        ccGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTa                                                                   >  1:118434/1‑150 (MQ=255)
                                                                         cGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTaa                                                                  <  2:36576/150‑1 (MQ=255)
                                                                           gggCGGATGACGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTaacc                                                                >  4:358787/1‑148 (MQ=255)
                                                                           gggCGGATGAAGATACACACTTACGCGCGACCAGCATGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGc                                                                >  2:285029/1‑150 (MQ=255)
                                                                            ggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGcc                                                               >  3:96732/1‑150 (MQ=255)
                                                                              gggATGGAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCgg                                                             <  2:576150/149‑1 (MQ=255)
                                                                                gATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGaa                                                           <  4:211543/150‑1 (MQ=255)
                                                                                  tGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAAcc                                                         >  2:144327/1‑150 (MQ=255)
                                                                                     aGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcg                                                      <  1:175267/150‑1 (MQ=255)
                                                                                         acacacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGTCTGTGCCGCCGGAGCGCAAAcg                                                                                                >  1:59463/1‑104 (MQ=255)
                                                                                         acacacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGTCTGTGCCGCCGGAGCGCAAAcg                                                                                                <  2:59463/104‑1 (MQ=255)
                                                                                           acacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCGCGCCGCAc                                                >  4:313875/1‑150 (MQ=255)
                                                                                              cTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTAt                                             <  2:166562/150‑1 (MQ=255)
                                                                                               ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                                                                                 >  4:390955/1‑97 (MQ=255)
                                                                                               ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                                                                                 <  3:390955/97‑1 (MQ=255)
                                                                                                tCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTg                                           <  3:243076/150‑1 (MQ=255)
                                                                                                 ccGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTgg                                          <  1:407943/150‑1 (MQ=255)
                                                                                                    cgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCt                                       <  1:144327/150‑1 (MQ=255)
                                                                                                    cgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCt                                       >  1:237084/1‑150 (MQ=255)
                                                                                                     gcgACCAGCCTGGAACCTCTGCGCACCATGGTGGCGGCAGGTAGCGAGATAACTTTCACGCCCACATAGTGTTTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTg                                      <  3:273268/150‑1 (MQ=255)
                                                                                                     gcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                                <  2:73263/108‑1 (MQ=255)
                                                                                                     gcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                                                                                >  1:73263/1‑108 (MQ=255)
                                                                                                     gcgACCAGCCTGGAAACTCTGCGCAACAGGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTg                                      <  3:346378/150‑1 (MQ=255)
                                                                                                       gACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGt                                    <  1:516481/150‑1 (MQ=255)
                                                                                                        aCCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGtt                                   >  2:263532/1‑150 (MQ=255)
                                                                                                          cAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTa                                 >  3:147840/1‑150 (MQ=255)
                                                                                                          cAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTa                                 <  4:270603/150‑1 (MQ=255)
                                                                                                            gCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATc                               <  2:242008/150‑1 (MQ=255)
                                                                                                             ccTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCg                              >  4:226353/1‑150 (MQ=255)
                                                                                                               tGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTc                            <  3:192819/150‑1 (MQ=255)
                                                                                                                  aaaCTCTGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACAGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTg                         <  1:475339/150‑1 (MQ=255)
                                                                                                                      tctGCGCAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTc                     >  4:220693/1‑150 (MQ=255)
                                                                                                                           gcAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGc                >  3:18958/1‑150 (MQ=255)
                                                                                                                            cAACATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCt               >  1:602972/1‑150 (MQ=255)
                                                                                                                               cATGGTGGCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTgcg            <  1:433169/150‑1 (MQ=255)
                                                                                                                                   gtggCGGCAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGc        >  1:333891/1‑150 (MQ=255)
                                                                                                                                       cggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCt    <  1:205353/150‑1 (MQ=255)
                                                                                                                                       cggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCt    <  1:340044/150‑1 (MQ=255)
                                                                                                                                       cggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGCCTCACCGCTGCGCAGCCGcc    >  4:371776/1‑149 (MQ=255)
                                                                                                                                        ggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTa   <  4:385530/150‑1 (MQ=255)
                                                                                                                                        ggcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTa   >  2:339451/1‑150 (MQ=255)
                                                                                                                                         gcAGGTAGCGAGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAt  >  3:42607/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTAT  >  NC_000913_3_pae_tpiA/4158965‑4159251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: