New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3781125	53 (0.900)	10 (0.180)	10/254	5.7	16.4%	intergenic (+108/‑90)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
	?	NC_000913_3_pae_tpiA	= 3781138	51 (0.900)					intergenic (+121/‑77)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAAT  >  NC_000913_3_pae_tpiA/3780989‑3781286                                                                                                                                                      |                                                                                                                                                     cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                                                                      <  2:115662/150‑1 (MQ=255)      aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCGTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTACCTCGCCCCTCCGGGGAGAggg                                                                                                                                                 >  3:343638/1‑150 (MQ=255)               gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                        <  4:235500/150‑1 (MQ=255)                      cggctcaGTCGTTTGCCCCCCCACACCCTCACCCTCTCCCTCAGGGAGTGTGGGCCGTACCGCGCACTATGTTCTCCCTCCCCCCAAACCGACCCCGAACTCTTTTTTTCTCCCTCGCCCCTCCCGCGAGCGGCCCGGAGTGAGGGGaaa                                                                                                                                 <  2:137176/144‑1 (MQ=255)                         cATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                                                                                                              <  1:258632/150‑1 (MQ=255)                                     cgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                                       <  2:248223/129‑1 (MQ=255)                                     cgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                                       >  1:248223/1‑129 (MQ=255)                                      gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGTGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgggagaaaa                                                                                                                                           <  3:157206/124‑7 (MQ=255)                                      gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGTGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgggagaaaa                                                                                                                                           >  4:157206/1‑118 (MQ=255)                                             cccccTACCCCCCCCCCGCGGGGAGGGGGGACCGTTGGGCGTTGTGTGTCCCCCCCCCCTCGGAAACGACCCCCCCCTTTTTTTTTCCCCCCCCCCCCGCGGGGGAGGGGCCGGGGGGGGGGGGAAAAGGCCGCACTGCTCACCAAtttt                                                                                                          <  2:155592/148‑1 (MQ=255)                                                ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                                       >  1:311745/1‑150 (MQ=255)                                                ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                                       >  1:186057/1‑150 (MQ=255)                                                      ccTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                 >  1:359464/1‑150 (MQ=255)                                                       ctctCCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                                >  3:257607/1‑150 (MQ=255)                                                        tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                               >  2:34461/1‑150 (MQ=255)                                                        tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                               >  3:235003/1‑150 (MQ=255)                                                        tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                               >  1:71507/1‑150 (MQ=255)                                                            cgatcgggAGGGGGGACGGTTCGGCGCGTTATGTTCCCCCCCACCCTAAACCGCCCCCGCACTCTTTTTTCCCCCCCCCCCCCCCCGGGGGGGGGGCGGGGGTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                                           <  2:439155/145‑1 (MQ=255)                                                              tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCACTCACTCTGAAACGACACCGCACTCTTTTTTTCTTCCGCGGCCCTCCGGTGTGTGGTCCTGGGTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                                                         >  1:133926/1‑150 (MQ=255)                                                                   gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccccagatcg                                                                                    >  1:439155/1‑144 (MQ=255)                                                                        gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                               >  2:581006/1‑150 (MQ=255)                                                                             cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                        <  1:584000/88‑1 (MQ=255)                                                                             cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                        >  2:584000/1‑88 (MQ=255)                                                                                   gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                                  >  3:381420/1‑72 (MQ=255)                                                                                   gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                                  <  4:381420/72‑1 (MQ=255)                                                                                   gcgcTGTATGTACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                                                    >  1:449662/1‑150 (MQ=255)                                                                                    cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                                                   >  1:40100/1‑150 (MQ=255)                                                                                            gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTTTTTTTCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACCACGGGGGCAGTATGCTAAACATCGTa                                                           >  2:145086/1‑150 (MQ=255)                                                                                            gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGCGAGGGCCGGGGCGAGGGGAAAAGTTCGCACTGGTGACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                           >  3:245590/1‑150 (MQ=255)                                                                                              aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                         >  1:387940/1‑150 (MQ=255)                                                                                              aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                         >  1:29628/1‑150 (MQ=255)                                                                                                cccccctctctGAACCGACACCGCACTTTTTTTTTCTCCCGCGCCCCTCGGGGGGGGGGGCGGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                       <  2:29628/143‑1 (MQ=255)                                                                                                        tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGTGGCAGTATGCTAAACATCGTACTTTACGAACCa                                               >  3:335489/1‑150 (MQ=255)                                                                                                          tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                                                                          >  2:270142/1‑57 (MQ=255)                                                                                                          tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                                                                          <  1:270142/57‑1 (MQ=255)                                                                                                                aacacCGCTCTTTTTTTTTCGCCGCCCCCCCCCGGGGGAGAGGGCCGGGGCGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                       <  2:501166/149‑1 (MQ=255)                                                                                                                            tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                      >  4:109988/1‑43 (MQ=255)                                                                                                                            tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                      <  3:109988/43‑1 (MQ=255)                                                                                                                                 ttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAca                      >  2:300695/1‑150 (MQ=255)                                                                                                                                  cccccccCGCCCCGCGGGGGGGGGGGCGGGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcat                     <  2:311745/147‑1 (MQ=255)                                                                                                                                     cccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat                  >  4:279026/1‑150 (MQ=255)                                                                                                                                      ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAgatcgga                 >  1:360222/1‑147 (MQ=255)                                                                                                                                      ccTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTCCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc                 >  3:316718/1‑150 (MQ=255)                                                                                                                                       cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                >  2:65037/1‑150 (MQ=255)                                                                                                                                        tctccccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                                                             >  2:438990/4‑104 (MQ=255)                                                                                                                                        tctccccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                                                             <  1:438990/101‑1 (MQ=255)                                                                                                                                            cccggaggggCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt           >  2:148466/7‑150 (MQ=255)                                                                                                                                              cTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg         >  4:22118/1‑150 (MQ=255)                                                                                                                                               tCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc        >  3:405484/1‑150 (MQ=255)                                                                                                                                               cccGGGGGGGGGGCGGGGGTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc        <  4:257607/149‑1 (MQ=255)                                                                                                                                                 cGGGGGGAGGCAAGGCGTGGGGAGAAAAGGCCGTGCTGCACACCAATTTTCTGCTATCCTGCCCCCGCACTACGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGACATTCCGCCAAATACTGGCAACATCATCCGTCTTTgcgc      <  2:133926/150‑1 (MQ=255)                                                                                                                                                  ggggggggggCGGGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCt     <  4:235003/150‑1 (MQ=255)                                                                                                                                                  ggggAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCt     >  4:143455/1‑150 (MQ=255)                                                                                                                                                   gggAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                                     >  1:136475/1‑117 (MQ=255)                                                                                                                                                   gggAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                                     <  2:136475/117‑1 (MQ=255)                                                                                                                                                    ggAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGTTaa   >  2:625034/1‑150 (MQ=255)                                                                                                                                                     gagaGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACAAATGTTCTGCTATCCTGCCCCCGCACTAAGGGTGCAGTTTGCTAAACATCGTACTTTACGAACCAGAAAGTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTaac  >  2:496763/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAAT  >  NC_000913_3_pae_tpiA/3780989‑3781286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.