Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,604,632	C→A	A186E (GCA→GAA)	bglJ →	bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,604,632	0	C	A	100.0%	265.9 / NA	78	A186E (GCA→GAA)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/35);  total (43/35)

ACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAC  >  NC_000913_3_pae_tpiA/4604495‑4604774                                                                                                                                           |                                                                                                                                               acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTa                                                                                                                                     >  1:1041911/1‑149 (MQ=255) acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGACGAAGTAAGATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTa                                                                                                                                     >  1:1043593/1‑149 (MQ=255)     aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgt                                                                                                                                 >  1:447028/1‑149 (MQ=255)             gTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGa                                                                                                                         >  2:873245/1‑149 (MQ=255)             gTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGa                                                                                                                         >  1:641606/1‑149 (MQ=255)              tGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCgca                                                                                                                        >  2:487696/1‑147 (MQ=255)              tGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGaa                                                                                                                        <  1:1105242/149‑1 (MQ=255)                   cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTgg                                                                                                                   >  1:44785/1‑149 (MQ=255)                   cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGGGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCCAAACGATCCGTGAACATAAATTTAATGTGATGTCTAAACTgc                                                                                                                   >  1:397206/1‑148 (MQ=255)                    aTTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTggg                                                                                                                  >  1:909182/1‑149 (MQ=255)                     atAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGGAGCTACTCAATGACACAAATG‑GCCGAGCAGCTTAACCGCAATATCAAAACGATCCGTGAACCTAAATTTAATGCGATGTCGAACCTGGGc                                                                                                                 >  2:837809/2‑149 (MQ=255)                       aaCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGt                                                                                                               <  1:323363/149‑1 (MQ=255)                         ccAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCa                                                                                                             <  2:1041911/149‑1 (MQ=255)                         ccAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCa                                                                                                             >  2:599447/1‑149 (MQ=255)                         ccAAAGCAGGACGTTTCGCTCGACGGAGATAGTAATATTTCGCGTTAGGTCTGGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCa                                                                                                             <  2:1043593/149‑1 (MQ=255)                          cAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg                                                                                                            >  2:317032/1‑149 (MQ=255)                           aaaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGt                                                                                                           >  1:748403/1‑149 (MQ=255)                              gCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt                                                                                                        <  2:641606/149‑1 (MQ=255)                                    cGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa                                                                                                  <  2:832707/149‑1 (MQ=255)                                     gTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgt                                                                                                                                 >  1:620831/1‑117 (MQ=255)                                     gTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgt                                                                                                                                 <  2:620831/117‑1 (MQ=255)                                          gCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCGGTTCTGACGCAGGgttg                                                                                            >  1:912369/1‑149 (MQ=255)                                            ccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAGTTCGAAACTGGGCGTCCGTTCTGACGCAGGggtgtt                                                                                          >  2:711935/1‑149 (MQ=255)                                             gggcGGAGGGAGAAATATTGCGCTGTAGGTCGCGTGGCTAGTCAATGACACAAATT‑GCCGAGCAGGTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg                                                                                         <  2:521061/146‑1 (MQ=255)                                              gACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGa                                                                                                                               <  2:895308/110‑1 (MQ=255)                                              gACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGa                                                                                                                               >  1:895308/1‑110 (MQ=255)                                              gACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGGCGGGGtggtgg                                                                                        >  1:97574/1‑149 (MQ=255)                                              gACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg                                                                                        >  1:97601/1‑149 (MQ=255)                                              gACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg                                                                                        <  2:44785/149‑1 (MQ=255)                                               ccGGAGAGAGAAAAATTGCGCTTTATGTCGCGTGTCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                       <  1:1031603/148‑1 (MQ=255)                                               aCGGAGAGAGAAATATTGGGTTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                       <  2:207334/149‑1 (MQ=255)                                                 ggAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAgg                                                                                     >  1:880321/1‑149 (MQ=255)                                                     agagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGc                                                                                 >  2:381852/1‑149 (MQ=255)                                                        gaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGa                                                                              >  1:790681/1‑149 (MQ=255)                                                         aaaTATTGCGCTTTCTCTCGAATCACTACTCAAGGACACAACTTCGGCGA‑CGGCGTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGat                                                                             <  1:1079991/149‑1 (MQ=255)                                                           atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGACCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAGTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGatat                                                                           <  2:41645/149‑1 (MQ=255)                                                              atGCGCTTTATGTTGAGTTGCTACTCAATGACACAAATT‑GCCGGGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATTTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt                                                                        <  1:672126/148‑1 (MQ=255)                                                                gcgcTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTgg                                                                                                                   >  1:342820/1‑104 (MQ=255)                                                                gcgcTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTgg                                                                                                                   <  2:342820/104‑1 (MQ=255)                                                                 cgcTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTTATCTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                     <  1:712246/149‑1 (MQ=255)                                                                  gcTTTATGTCGCGTGGCTACTAAATGACACAAATT‑GCCGAGCAGCTTAAACGAGATATCAAAACGATGCGTGAACATAAATTTAAAGTGATGTCGAAACCGGGCGGCAGTTCTGACGCGGGGGTGGTGAAGGCCGCACAAATTCTTTta                                                                    >  2:923439/1‑149 (MQ=255)                                                                   cgttATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAACCGCAATATCAAAACGATCCGTGAACATAACTTTAATGTGATGTGGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGCCCGCAGCTATTCTGTtat                                                                   >  2:427434/3‑149 (MQ=255)                                                                   cTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtat                                                                   <  1:615128/149‑1 (MQ=255)                                                                    tttATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg                                                                                         >  2:690932/1‑126 (MQ=255)                                                                    tttATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg                                                                                         <  1:690932/126‑1 (MQ=255)                                                                       aTGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtat                                                               <  1:381852/149‑1 (MQ=255)                                                                          tCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCg                                                            >  1:1072643/1‑149 (MQ=255)                                                                               tGGCTAGCCAAAGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGAGCCGTGAACATAAATTTAATGTGATGTCGAATGTGGGCGTCATTTCTGACGTAGTGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAtt                                                       <  2:235306/149‑1 (MQ=255)                                                                                      tCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc                                                >  2:782485/1‑149 (MQ=255)                                                                                      tCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc                                                >  2:674563/1‑149 (MQ=255)                                                                                        aaTGACATAAATT‑GCCGAGCAGCTTAAACGCAATAACAAAACGATCCGTGAACATATGTCTAAGGGTATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa                                              <  1:257938/149‑1 (MQ=255)                                                                                         aTGACACAAATT‑GCCGAGCAGCCTAAACGCAATATCACAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAg                                             <  1:185340/149‑1 (MQ=255)                                                                                         aTGACACAAATT‑GCCGAGAAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAg                                             <  1:184262/149‑1 (MQ=255)                                                                                           gACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTa                                           <  2:193424/149‑1 (MQ=255)                                                                                             cacaAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGTGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                         <  2:600513/149‑1 (MQ=255)                                                                                             cacaAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                         >  1:487758/1‑149 (MQ=255)                                                                                              acaAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg                                        >  2:832310/1‑149 (MQ=255)                                                                                                aaaTT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgtg                                      >  1:142657/1‑149 (MQ=255)                                                                                                  aTT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                    >  1:486391/1‑149 (MQ=255)                                                                                                           gcagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAc                                                                                                     <  2:825637/76‑1 (MQ=255)                                                                                                           gcagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAc                                                                                                     >  1:825637/1‑76 (MQ=255)                                                                                                              gcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCGTTGCGACACAAGTAATGTGTTGCATCCCTAtt                         >  2:315067/1‑149 (MQ=255)                                                                                                                ttAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTaa                       >  2:709881/1‑149 (MQ=255)                                                                                                                  aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgt                                       >  1:732669/1‑131 (MQ=255)                                                                                                                  aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgt                                       <  2:732669/131‑1 (MQ=255)                                                                                                                       cAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                <  1:709881/149‑1 (MQ=255)                                                                                                                        aaTATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAATTCTGACGCAGGGTAGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATTCATCTTAATCCGCatg               >  2:172328/1‑149 (MQ=255)                                                                                                                           atCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCatgatg            <  2:880321/149‑1 (MQ=255)                                                                                                                              aaaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAAGTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCg         <  2:909182/149‑1 (MQ=255)                                                                                                                              aaaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCg         >  1:290057/1‑149 (MQ=255)                                                                                                                              aaaaCGATCCGTGAACATAAATTTAATGTGATGACGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTTTGAATCAGTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGCTGCCg         >  1:810030/1‑149 (MQ=255)                                                                                                                                aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                >  2:15462/1‑140 (MQ=255)                                                                                                                                aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                <  1:15462/140‑1 (MQ=255)                                                                                                                                    aTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa   <  1:699574/149‑1 (MQ=255)                                                                                                                                    aTCCGTGAACAGAAAAATGAGGTATTGTCGGAACTGTGCGTCAGTTCTGAAGCAGGGATGTTTGCGGCCGCAGATATTTTGTTATTTATGCGGCATGTCTAAACAAGTAATGTGTTGCAGCCCTTCTCATCCGCATGATGCCGGGATTa   >  2:904813/1‑149 (MQ=255)                                                                                                                                     tCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc  >  2:78494/1‑149 (MQ=255)                                                                                                                                     tCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc  <  2:611820/149‑1 (MQ=255)                                                                                                                                     tCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc  <  2:611805/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                               ACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATT‑GCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAC  >  NC_000913_3_pae_tpiA/4604495‑4604774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑