Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	482,230	0	G	A	100.0%	250.1 / NA	74	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/34);  total (40/34)

TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAA  >  NC_000913_3_pae_tpiA/482084‑482365                                                                                                                                                    |                                                                                                                                        tgacatAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCagg                                                                                                                                       <  2:54216/149‑1 (MQ=255)     atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTAt                                                                                                                                   >  1:808693/1‑149 (MQ=255)      tAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATc                                                                                                                                  >  1:1050130/1‑149 (MQ=255)           tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCa                                                                                                                             >  1:667569/1‑149 (MQ=255)           tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCa                                                                                                                             <  1:917286/149‑1 (MQ=255)           tttCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGCTTCTTACa                                                                                                                             >  2:1102691/1‑149 (MQ=255)                  acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTc                                                                                                                               >  1:502504/1‑140 (MQ=255)                  acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTc                                                                                                                               <  2:502504/140‑1 (MQ=255)                  acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                      >  2:84016/1‑149 (MQ=255)                  acacGACCGCGGTCGATAAAGTCGTTCACATAGCCCCCACATCTTTCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGtt                                                                                                                      >  1:542886/1‑149 (MQ=255)                   cacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGttt                                                                                                                     <  2:1048344/149‑1 (MQ=255)                     cGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGCCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTgg                                                                                                                   <  2:612166/149‑1 (MQ=255)                      gaagccgGTGGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                  <  2:52143/144‑1 (MQ=255)                      gACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                  >  1:307157/1‑149 (MQ=255)                           cgGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAc                                                                                                             <  2:76718/149‑1 (MQ=255)                            ggTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACg                                                                                                            >  2:525191/1‑149 (MQ=255)                              ttgATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCt                                                                                                          <  2:209863/147‑1 (MQ=255)                              tCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCt                                                                                                          >  2:294000/1‑149 (MQ=255)                                   aaaGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGAACGTTTGGATGTACGCTGGTCa                                                                                                     >  2:671764/1‑149 (MQ=255)                                     aGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCGAAAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGCACGTTTGGCCGTACGCTGGCCAAc                                                                                                   >  2:238578/1‑149 (MQ=255)                                     aGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTTTTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAAc                                                                                                   <  1:489347/149‑1 (MQ=255)                                          ttCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATc                                                                                              >  1:329277/1‑149 (MQ=255)                                             acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                           >  2:407019/1‑149 (MQ=255)                                             acaTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAgg                                                                                           <  2:307157/149‑1 (MQ=255)                                               aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGCAACACCCACCGCCTGCGATTACCCCTGGTCG‑CATCACGTCTTATCCAGAAAGGTCTCCACCAGACTCTAGGTACGTACGCTTGTCAACATATCACGGt                                                                                         >  2:96674/1‑149 (MQ=255)                                                tAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAACCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGTCGTACGCTGGTCAACATATCAGGGTg                                                                                        >  2:1073585/1‑149 (MQ=255)                                                  acTGGCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAGACTGCAGGGTATCTTCCAGACCGTTTGGATGTACGCTGGTCAACATATCAGGGTGCt                                                                                      >  1:1049119/2‑149 (MQ=255)                                                    tGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTc                                                                                    <  1:464290/149‑1 (MQ=255)                                                    tGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTc                                                                                    >  1:518681/1‑149 (MQ=255)                                                           ccATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACATCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCtt                                                                             <  1:487796/149‑1 (MQ=255)                                                                 aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGCAACGCCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                       <  1:859290/149‑1 (MQ=255)                                                                 aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                  <  1:84181/106‑1 (MQ=255)                                                                 aGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                  >  2:84181/1‑106 (MQ=255)                                                                 aGCGCCCACAGGTGTGTTAATTTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTgcaa                                                                       <  2:313991/149‑1 (MQ=255)                                                                          agTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                  <  1:195813/97‑1 (MQ=255)                                                                          agTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGa                                                                                                                  >  2:195813/1‑97 (MQ=255)                                                                           gtggtgTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGtt                                                             <  1:407019/149‑1 (MQ=255)                                                                               tgtTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTAcgc                                                         <  2:329277/149‑1 (MQ=255)                                                                                 ttAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCCCTGCTTCTGCAAGCAACTGGTTACgcgc                                                       >  1:264570/1‑149 (MQ=255)                                                                                   aaTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCt                                                     >  2:171012/1‑149 (MQ=255)                                                                                    aTGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACATACGCTGATCAACATATCAGGGTGCTTCCCTGCTTCTGCAAGCAACTGGTTACCCGCCta                                                    >  1:233323/1‑148 (MQ=255)                                                                                     tGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGa                                                   >  2:679076/1‑149 (MQ=255)                                                                                             aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCATGTGATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                           >  1:1043716/1‑149 (MQ=255)                                                                                             aTAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCCGttt                                           >  2:858633/1‑149 (MQ=255)                                                                                                gAAACACCCAGCGCCTGCGCTTTCTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCTACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                        <  1:933587/149‑1 (MQ=255)                                                                                                 aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGAcc                                                                                                                         <  1:230677/67‑1 (MQ=255)                                                                                                 aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGAcc                                                                                                                         >  2:230677/1‑67 (MQ=255)                                                                                                 aaaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTACg                                       >  2:329392/1‑149 (MQ=255)                                                                                                  aaCACCCAGCGCCTGCTCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                      <  1:239911/149‑1 (MQ=255)                                                                                                  aaCACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                      <  2:812995/149‑1 (MQ=255)                                                                                                    cacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa                                    <  2:667569/149‑1 (MQ=255)                                                                                                     acCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACAGTTTGGACGTACGATGGTCAACATATTAGGCTGCTTCTCTGATTCTGAAAGGAACTGGTTACGCGCCTGAGTAAGTTTTTAGTGAc                                   >  2:537528/1‑149 (MQ=255)                                                                                                       ccAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                 >  2:327123/1‑149 (MQ=255)                                                                                                        cAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCa                                                                                                                             >  2:992021/1‑56 (MQ=255)                                                                                                        cAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCa                                                                                                                             <  1:992021/56‑1 (MQ=255)                                                                                                        cAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa                                <  2:872073/149‑1 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTGGTCGATATCC‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                               <  1:758375/149‑1 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                               >  1:652433/1‑149 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                               <  1:755102/149‑1 (MQ=255)                                                                                                         aGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                               <  1:525191/149‑1 (MQ=255)                                                                                                             ccTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCCACATCTCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAACAAGCCCa                           >  2:375483/1‑149 (MQ=255)                                                                                                             ccTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATGAGGGTGCTTCGCTGCTTCTGCAAGCAACTGATTACGCTCCTGAGTCAGTTTTTCGTGGCCACGGccc                           >  2:13902/1‑148 (MQ=255)                                                                                                               tGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc                         >  2:890445/1‑149 (MQ=255)                                                                                                                  gcTGTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                      <  1:580055/149‑1 (MQ=255)                                                                                                                   cTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                     <  1:171012/149‑1 (MQ=255)                                                                                                                   cTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCCCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTgg                     >  1:344003/1‑149 (MQ=255)                                                                                                                    tttttCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTc                                                                                    <  1:954299/85‑1 (MQ=255)                                                                                                                    tttttCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTc                                                                                    >  2:954299/1‑85 (MQ=255)                                                                                                                    tttttCCTGGTCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt                    >  1:318745/1‑149 (MQ=255)                                                                                                                        tCCTGGGCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                <  2:128563/149‑1 (MQ=255)                                                                                                                              tCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc          >  1:691949/1‑149 (MQ=255)                                                                                                                              tCGATATCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc          <  1:890445/149‑1 (MQ=255)                                                                                                                                 atatCA‑ATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa       <  2:233190/149‑1 (MQ=255)                                                                                                                                 atagCA‑ATCGTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTAGGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa       <  2:222671/149‑1 (MQ=255)                                                                                                                                      a‑aTCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCCATCAGCtccaagtcaa  >  1:407985/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                        TGACATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCA‑ATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAA  >  NC_000913_3_pae_tpiA/482084‑482365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑