New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 364812754 (0.850)11 (0.190) 11/236 5.5 19.4% noncoding (65/84 nt) agrA inactive antisense sRNA
?NC_000913_3_pae_tpiA = 3648363 42 (0.720)noncoding (70/84 nt) agrB antisense sRNA antitoxin for the DinQ toxin
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGG  >  NC_000913_3_pae_tpiA/3647980‑3648276
                                                                                                                                                   |                                                                                                                                                     
aaCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGActt                                                                                                                                                     >  3:450024/1‑148 (MQ=255)
   tGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCaaa                                                                                                                                                  <  1:85954/150‑1 (MQ=255)
        aaTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCgg                                                                                                                                             >  2:521264/1‑150 (MQ=255)
          tCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtt                                                                                                                                           >  4:287628/1‑150 (MQ=255)
             tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttt                                                                                                                                        <  4:11731/150‑1 (MQ=255)
                     aTGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATcc                                                                                                                                <  1:399299/150‑1 (MQ=255)
                       gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCaa                                                                                                                                                   <  3:335135/129‑1 (MQ=255)
                       gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCaa                                                                                                                                                   >  4:335135/1‑129 (MQ=255)
                           gTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTc                                                                                                                          <  2:316523/150‑1 (MQ=255)
                               taAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGAc                                                                                                                      <  2:415579/150‑1 (MQ=255)
                                  aaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAg                                                                                                                   <  1:521264/150‑1 (MQ=255)
                                   aCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAgg                                                                                                                  <  3:287628/150‑1 (MQ=255)
                                    ccggCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGc                                                                                                                 <  3:393773/147‑1 (MQ=255)
                                    ccAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGAAAAGGc                                                                                                                 >  1:571557/1‑150 (MQ=255)
                                           gTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAatat                                                                                                          >  4:376869/1‑150 (MQ=255)
                                             ccTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                      <  1:128579/120‑1 (MQ=255)
                                             ccTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                      >  2:128579/1‑120 (MQ=255)
                                                   aaTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGa                                                                                                  <  1:653646/150‑1 (MQ=255)
                                                            aGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaa                                                                                         <  1:7566/150‑1 (MQ=255)
                                                            aGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaa                                                                                         >  1:525559/1‑150 (MQ=255)
                                                              tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                       >  1:432473/1‑150 (MQ=255)
                                                               aTGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGt                                                                                      <  3:380513/150‑1 (MQ=255)
                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                      <  3:75038/150‑1 (MQ=255)
                                                                                gcattAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAATAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAgg                                                                                                                  <  2:517544/102‑1 (MQ=255)
                                                                                gCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAgg                                                                                                                  >  1:517544/1‑105 (MQ=255)
                                                                                 cGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCg                                                                    >  3:377010/1‑150 (MQ=255)
                                                                                 cGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCg                                                                    <  4:212897/150‑1 (MQ=255)
                                                                                             gCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGaa                                                        >  2:743462/1‑150 (MQ=255)
                                                                                              cTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                       >  1:697267/1‑150 (MQ=255)
                                                                                                     aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGCCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTaa                                                                                                             <  1:3102/89‑1 (MQ=255)
                                                                                                     aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTaa                                                                                                             >  2:3102/1‑89 (MQ=255)
                                                                                                       tCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                            <  3:5248/120‑1 (MQ=255)
                                                                                                       tCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                            >  4:5248/1‑120 (MQ=255)
                                                                                                        ccAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccctcc                                             >  4:28065/1‑150 (MQ=255)
                                                                                                          aGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGc                                           <  1:743462/150‑1 (MQ=255)
                                                                                                            tCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                        <  2:80189/103‑1 (MQ=255)
                                                                                                            tCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                        >  1:80189/1‑103 (MQ=255)
                                                                                                                  ccaccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaa                                   <  4:211994/150‑1 (MQ=255)
                                                                                                                  ccaccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaa                                   >  3:211994/1‑150 (MQ=255)
                                                                                                                     ccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                            <  1:103726/106‑1 (MQ=255)
                                                                                                                     ccaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                            >  2:103726/1‑106 (MQ=255)
                                                                                                                              ggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                            <  1:41607/97‑1 (MQ=255)
                                                                                                                              ggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                            >  2:41607/1‑97 (MQ=255)
                                                                                                                                 tGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCt                    >  1:305546/1‑150 (MQ=255)
                                                                                                                                 tGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCt                    >  4:195813/1‑150 (MQ=255)
                                                                                                                                   gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGa                                                                                                        <  4:171441/64‑1 (MQ=255)
                                                                                                                                   gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGa                                                                                                        >  3:171441/1‑64 (MQ=255)
                                                                                                                                   gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                  <  3:376869/150‑1 (MQ=255)
                                                                                                                                          aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATAGAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt           >  2:515505/1‑150 (MQ=255)
                                                                                                                                             aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGc        <  2:525559/150‑1 (MQ=255)
                                                                                                                                                 gACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt    <  4:318276/150‑1 (MQ=255)
                                                                                                                                                  aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCa                                                                         <  4:206185/80‑1 (MQ=255)
                                                                                                                                                  aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCa                                                                         >  3:206185/1‑80 (MQ=255)
                                                                                                                                                  aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTg   >  1:729214/1‑150 (MQ=255)
                                                                                                                                                   cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg  >  3:133701/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGG  >  NC_000913_3_pae_tpiA/3647980‑3648276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.