New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	4139396 =	29 (0.740)	4 (0.110)	4/270	5.2	11.7%	coding (229/663 nt)	fsaB	fructose‑6‑phosphate aldolase 2
	?	NC_000913_3_pae_tpiA	4139434 =	33 (0.900)					coding (191/663 nt)	fsaB	fructose‑6‑phosphate aldolase 2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCG  >  NC_000913_3_pae_tpiA/4139255‑4139539                                                                                                                                              |                                                                                                                                                cTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAgg                                                                                                                                          >  2:453354/1‑149 (MQ=255) cTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAgg                                                                                                                                          >  1:380447/1‑149 (MQ=255)    ccAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGgcgc                                                                                                                                       <  1:252506/149‑1 (MQ=255)      aGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgctt                                                                                                                                     >  1:573051/1‑149 (MQ=255)         gCGGCTAATAACCCTTGTGCGGCGGTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgcttcgc                                                                                                                                  <  2:564196/149‑1 (MQ=255)                         gtgCGGCACTATAAACAGCAGTCCCAAGTCAATAAATACCCTCTTTTTTCACTATATTAATTGCTGACATACCTACATAAGTAACCCAGATTTTCACCACAATACCCGGAATAGCTTCGCTCATTCGCTTCGCTTCTTCCACCATcccc                                                                                                                  <  2:308806/149‑1 (MQ=255)                          tgtggcgCTATATACAGCAGTGCGAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCCCAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCTTCCCCt                                                                                                                 <  2:573126/146‑1 (MQ=255)                                     ataCCGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCgg                                                                                                      <  1:140231/149‑1 (MQ=255)                                      taCAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGc                                                                                                     >  1:486871/1‑149 (MQ=255)                                         agcagTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTGCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCCCa                                                                                                  >  2:188247/1‑149 (MQ=255)                                             gTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCAGCCCCTGCGCGTCGCGGCTCATGGt                                                                                              <  2:278182/149‑1 (MQ=255)                                              tGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTc                                                                                             >  1:476836/1‑149 (MQ=255)                                              tGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGACAGTCACCGGGATTGTCCCCACAATACCCGGAATAGCGTCGCGCGGGCGCTGCGCTTCTTCCACCATCCCCAGCGCGTCGCGGCTCATggac                                                                                             >  1:147895/1‑147 (MQ=255)                                                                    ttttttCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCtcat                                                                       >  2:668897/1‑149 (MQ=255)                                                                      gtttCAGTATTTTAATTGCTGCCAGACCTTAGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCtcatca                                                                     <  2:525700/148‑1 (MQ=255)                                                                            gTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGGGCAAACAGAATGCCCTCATCACCAATc                                                               <  2:670742/149‑1 (MQ=255)                                                                                   aaTTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCACCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCttttt                                                        <  1:304839/149‑1 (MQ=255)                                                                                         tgcCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGaa                                                  >  1:217954/1‑148 (MQ=255)                                                                                           ccAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAACCGCTTTTTGAAGAcgc                                                >  2:200443/1‑149 (MQ=255)                                                                                                  ttCGGAAGTCACCGGGATTTTCCCCACAATGCCCGGAATAGCGTCGCGCAGGCGGTTCGCTACTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGggagca                                         <  2:519122/149‑1 (MQ=255)                                                                                                            aCCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCACGTCGCGGCTCCTGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCatat                               >  2:640957/1‑149 (MQ=255)                                                                                                             ccGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATg                              <  1:140089/149‑1 (MQ=255)                                                                                                             ccGGGATTGTCACCACAACAGCCGGCATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGATTTTTGCAGACGCGGCAGCACTTCCCATATg                              <  2:529624/149‑1 (MQ=255)                                                                                                              cGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATgg                             >  1:277676/1‑149 (MQ=255)                                                                                                               gggATTTTCACCACACTACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGa                            <  1:128432/149‑1 (MQ=255)                                                                                                                          cacaATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTgg                 >  2:355312/1‑149 (MQ=255)                                                                                                                          cacaATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTgg                 <  2:573051/149‑1 (MQ=255)                                                                                                                                 cccGCAATAGGGTCGCGCAGGCGCTTCGCTGCTTCCACCATCGCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGTTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGAt          <  2:102245/149‑1 (MQ=255)                                                                                                                                         aGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCg  <  2:44625/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                CTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCG  >  NC_000913_3_pae_tpiA/4139255‑4139539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.