| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 1,197,502 | C→T | G11R (GGA→AGA) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,197,502 | 0 | C | T | 97.0% | 92.5 / NA | 33 | G11R (GGA→AGA) | ymfD | e14 prophage; putative SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/18); minor base G (0/1); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGA > NC_000913_3_pae_tpiA/1197364‑1197646 | aTTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGCGCAtatta > 2:219051/1‑149 (MQ=255)aTTCATCAGAATATCTAAGTTTTCCCCAGCCAAAATCAAGGGCGTGTCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGTTTTTGCTGCATTCTCTGGGCGAATATTAACTCTGTGCAtatta < 2:360429/149‑1 (MQ=255) tcatcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAACGTTTATACTTTACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATtata < 2:417571/149‑1 (MQ=255) tcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTCATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGtt > 1:268947/1‑149 (MQ=255) caGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTc < 1:573944/149‑1 (MQ=255) caGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTc < 1:573857/149‑1 (MQ=255) aaTATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGAATCTAATATACTCGAAAAGATATCTAGATGACATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCaaa < 1:647423/149‑1 (MQ=255) tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAAGTTTTGCTACATTCTCTGAGCGAATATTAACTCTGTGGATATTATAGTTTAAATCAAGt > 2:371955/1‑149 (MQ=255) ttttCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCa > 2:448527/1‑149 (MQ=255) aaGGGCGTGGCCATTTTTCTCAATGCTTCAAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTAtttt < 1:637283/149‑1 (MQ=255) gggCGGTGCTATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCGCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTa < 1:266670/149‑1 (MQ=255) aTTTTTCTCAAGGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACGCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAAtt > 1:420418/1‑149 (MQ=255) ctcATTGCTTCTAATATACTCGCAAAGACATTTAGAGGGCAACGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTGTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCtttta < 2:198622/149‑1 (MQ=255) tcAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttttat > 1:311201/1‑149 (MQ=255) gttttAATATACTCGCAAAGATATCTAGAGGTCAACGTATGATGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATc < 2:653187/145‑1 (MQ=255) ataCTCGAAAAAAAATATAAAGGGTATCGTATGAGGTTTTGATGCATTCTATGAGCGAATATTAACTCTCTGCATATTATAGTACAAATCAAGTACCATTTTTTTAATGTAAAAAAAAAAAAAAAATTCTTTTATTTTATCGTCCGGGa < 2:580444/149‑1 (MQ=255) gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTTCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTc > 1:165632/1‑149 (MQ=255) aGATATTTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTCAAAACTTCTTTTATTTTATCGTCCGGGATTTTTCCgac < 2:116463/149‑1 (MQ=255) aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCgac < 1:448527/149‑1 (MQ=255) aGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGAATTTTTCCgac > 2:545893/1‑149 (MQ=255) tCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCgacttgac > 2:325823/1‑149 (MQ=255) agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACttt < 1:209360/149‑1 (MQ=255) gagGGCATCTTATGAGGTGTGGCTGCATTCTCTGGGCGAATATTAACTCTGTGCAGATTATAGTTCGAAGCAAGTACCATTCAGTTAAGGTTATTTTTAGTGAAGAGTACTTTCATTTTATCGTCCGGGATTTTTCGTACTTGACTTTc > 2:540704/1‑149 (MQ=255) tGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCtt > 1:359527/1‑149 (MQ=255) tGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGAATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTACGGGATTTTTCCGACTTGACTTTCAGTTCTGGGGtt > 1:263903/1‑149 (MQ=255) aGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTaa < 2:372935/149‑1 (MQ=255) ttttGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttttat < 2:25289/99‑1 (MQ=255) ttttGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCttttat > 1:25289/1‑99 (MQ=255) tcgctGAGCGAATATTAACTCTGTGTATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTTTTTTATTTTATCGTCCGGGATTTTTTCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaaag < 2:280720/146‑1 (MQ=255) gtctGAGCGAAGATGAACTTGGCGTATATTATAGTTCAAAGCATGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTATTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGa < 2:70471/148‑1 (MQ=255) tctGAGCGTATATTAACGCTGTGCATATTATAGTGCAAAGCAAGTACCATTCAAATAATGTTATTTTTAGTGAAAAATCCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGat < 1:418631/149‑1 (MQ=255) aaTATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTGTTGAAACGGCCGGGATCCATCGTTCTTTTCTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACcac > 1:130305/1‑149 (MQ=255) aCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTga < 1:219051/149‑1 (MQ=255) | ATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGA > NC_000913_3_pae_tpiA/1197364‑1197646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |