Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,112,1900AC58.9% 142.0 / 207.7 159intergenic (‑60/‑41)yiiQ/yiiRDUF1454 family putative periplasmic protein/DUF805 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (39/26);  new base C (51/42);  total (91/68)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 165 total aligned reads displayed.

GTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTG  >  NC_000913_3_pae_tpiA/4112042‑4112338
                                                                                                                                                    |                                                                                                                                                    
 ttGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAg                                                                                                                                                    >  2:616512/1‑150 (MQ=255)
   gCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAggg                                                                                                                                                  <  4:220961/150‑1 (MQ=255)
          cTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCGTGTCAAAAGGGTAGAATc                                                                                                                                           <  2:639981/150‑1 (MQ=255)
              tgtTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTgg                                                                                                                                       <  2:50601/150‑1 (MQ=255)
               gtTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGa                                                                                                                                      >  4:609618/1‑150 (MQ=255)
                  tgtgCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGaaaa                                                                                                                                   <  1:187533/150‑1 (MQ=255)
                  tgtgCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGaaaa                                                                                                                                   <  2:243180/150‑1 (MQ=255)
                   gtgCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGa                                                                                                                                              >  1:261017/1‑138 (MQ=255)
                        tGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAAc                                                                                                                             <  4:41135/150‑1 (MQ=255)
                           gcCTCTGGTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCAt                                                                                                                          >  2:574578/1‑150 (MQ=255)
                              tctGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCaa                                                                                                                       >  3:34321/1‑150 (MQ=255)
                              tctGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCAATGATTAAAAAGATATATGTTGAAATTTTCAAGAAGGTATAATCATGGAAAAGACAACCATCaa                                                                                                                       >  1:460186/1‑150 (MQ=255)
                                tgttgtAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGCCAGTACCTTTTGTTATGAATGGCTTCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAgg                                                                                                                     <  4:448440/150‑1 (MQ=255)
                                     tAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAgg                                                                                                                     <  2:20299/145‑1 (MQ=255)
                                     tAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACtt                                                                                                                >  1:5470/1‑150 (MQ=255)
                                        cGGTTAATGCAAAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGTCAACCATCAAGGAACTTACa                                                                                                             <  4:47634/150‑1 (MQ=255)
                                         ggTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACAt                                                                                                            <  1:329078/150‑1 (MQ=255)
                                          gTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTAGCTCGTAGTAAAACTTTATCTAGGGCCCTAGTCGAAAGGGTATAATCCGGGAAAAGACAGCCATCAAGGAACTTACAtt                                                                                                           >  1:776196/1‑149 (MQ=255)
                                              aTGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCa                                                                                                       >  4:514605/1‑150 (MQ=255)
                                                   gAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACt                                                                                                                 >  1:707972/1‑135 (MQ=255)
                                                   gAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTa                                                                                                               >  2:954736/1‑137 (MQ=255)
                                                   gAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTa                                                                                                               <  1:954736/137‑1 (MQ=255)
                                                           aagaAAAAACATCGTACTCCCTGGCTGCATTGACAGTACCTTGTGTTATGGCTGGCGCCGATTAATACGATATCTGTCGCCCTTGTCAGCAGGGTAGAATCCTGGAAAAGACCACCATCAAGGAGCTTACATGACCCTACAGGAATGGtt                                                                                          <  4:619290/149‑1 (MQ=255)
                                                              aaaaaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTAtt                                                                                       >  1:979587/1‑150 (MQ=255)
                                                              aaaaaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTAtt                                                                                       >  1:236621/1‑150 (MQ=255)
                                                              aaaaaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTAtt                                                                                       >  4:435110/1‑150 (MQ=255)
                                                                aaaaCAGCGTACACCCTGGCTTCATTGCCAGTACCTTTTGTTATGACCGGCTAAGATTCAATCGAATTACGATGTCTTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATtct                                                                                     <  1:616512/150‑1 (MQ=255)
                                                                aaaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATtct                                                                                     >  2:852218/1‑150 (MQ=255)
                                                                 aaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATtctc                                                                                    >  3:76297/1‑150 (MQ=255)
                                                                 aaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATtctc                                                                                    >  1:791851/1‑150 (MQ=255)
                                                                 aaaCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAgg                                                                                                                                                   <  2:247983/87‑1 (MQ=255)
                                                                 aaaCAGCATACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATtctc                                                                                    >  2:1028223/1‑150 (MQ=255)
                                                                   aCAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCAt                                                                                  <  1:21348/150‑1 (MQ=255)
                                                                         tACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAg                                                                            >  4:421538/1‑150 (MQ=255)
                                                                         tACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAg                                                                            <  4:630760/150‑1 (MQ=255)
                                                                          acacGCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGGCGCCCTTGTCAAAAGGGTAGAATCCTGAAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAgg                                                                           >  4:168275/1‑150 (MQ=255)
                                                                          acacCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAgg                                                                           >  2:264935/1‑150 (MQ=255)
                                                                           cacCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAAcc                                                                                                                            <  2:358580/100‑1 (MQ=255)
                                                                           cacCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAAcc                                                                                                                            >  1:358580/1‑100 (MQ=255)
                                                                           cacCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAggg                                                                          <  3:609618/150‑1 (MQ=255)
                                                                           cacCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAggg                                                                          <  1:662921/150‑1 (MQ=255)
                                                                               cTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTa                                                                      <  3:260325/150‑1 (MQ=255)
                                                                                tGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTAt                                                                     >  1:674796/1‑150 (MQ=255)
                                                                                 ggCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTAtt                                                                    <  1:1028223/150‑1 (MQ=255)
                                                                                   cTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTgg                                                                  >  2:70848/1‑150 (MQ=255)
                                                                                     tCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGAc                                                                <  3:641654/150‑1 (MQ=255)
                                                                                     tCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGAc                                                                >  1:765866/1‑150 (MQ=255)
                                                                                     tCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGa                                                                                                                    <  1:649895/98‑1 (MQ=255)
                                                                                     tCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGa                                                                                                                    >  2:649895/1‑98 (MQ=255)
                                                                                       aTTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGc                                                              >  2:749329/1‑150 (MQ=255)
                                                                                          gACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGt                                                           <  3:192529/150‑1 (MQ=255)
                                                                                             aGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGGAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGAt                                                        >  1:190156/1‑150 (MQ=255)
                                                                                               tACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGAttt                                                      >  3:462750/1‑150 (MQ=255)
                                                                                                 ccTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCt                                                    <  2:5470/150‑1 (MQ=255)
                                                                                                   ttttGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTgg                                                  >  3:555953/1‑150 (MQ=255)
                                                                                                   ttttGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTgg                                                  <  3:32608/150‑1 (MQ=255)
                                                                                                    tttGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTg                                                          <  2:613248/141‑1 (MQ=255)
                                                                                                    tttGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTg                                                          >  1:613248/1‑141 (MQ=255)
                                                                                                       gTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCATGATTTCTGGAttt                                              >  4:348635/1‑150 (MQ=255)
                                                                                                         tATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTa                                                                                                                                                >  2:221608/1‑50 (MQ=255)
                                                                                                           tGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACt                                                                                                                 >  1:1016475/1‑79 (MQ=255)
                                                                                                           tGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACt                                                                                                                 <  2:1016475/79‑1 (MQ=255)
                                                                                                            gACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATa                                         >  1:920995/1‑150 (MQ=255)
                                                                                                             aCTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAg                                        <  2:812831/150‑1 (MQ=255)
                                                                                                             aCTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAg                                        >  2:944098/1‑150 (MQ=255)
                                                                                                               tGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGc                                      >  2:296417/1‑150 (MQ=255)
                                                                                                                 gCTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATCCAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATGTCTGGATTTGGATAGGCCt                                    >  2:811663/1‑150 (MQ=255)
                                                                                                                 gCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCt                                    >  3:208913/1‑150 (MQ=255)
                                                                                                                  cTCCGATTAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCtg                                   <  3:141400/150‑1 (MQ=255)
                                                                                                                  cTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTGAAAGGGCGTATTGGACGCCGTGCTTTCGCAGAGTGGATAGGCCtg                                   >  1:574812/1‑150 (MQ=255)
                                                                                                                         tAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCt                                                                                                                                         <  2:210552/41‑1 (MQ=255)
                                                                                                                         tAAAACGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCt                                                                                                                                         >  1:210552/1‑41 (MQ=255)
                                                                                                                           aaaCGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTgg                                                  <  1:541795/126‑1 (MQ=255)
                                                                                                                           aaaCGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCAttt                                                                                <  3:561707/96‑1 (MQ=255)
                                                                                                                            aaCGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAg                         >  2:506809/1‑150 (MQ=255)
                                                                                                                            aaCGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAAGGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAg                         >  1:880056/1‑150 (MQ=255)
                                                                                                                            aaCGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                                                                                                 >  4:35055/1‑78 (MQ=255)
                                                                                                                            aaCGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAg                         >  4:457858/1‑150 (MQ=255)
                                                                                                                            aaCGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAg                         <  1:44282/150‑1 (MQ=255)
                                                                                                                             aCGCTATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                           >  4:502164/1‑131 (MQ=255)
                                                                                                                             aCGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGa                                           <  3:502164/131‑1 (MQ=255)
                                                                                                                             aCGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAgg                        >  1:286112/1‑150 (MQ=255)
                                                                                                                              cGATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTAc                                                                                                              <  2:855933/63‑1 (MQ=255)
                                                                                                                               gATATCTGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCa                      >  1:804390/1‑150 (MQ=255)
                                                                                                                                  atCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAtt                                               <  2:126582/122‑1 (MQ=255)
                                                                                                                                  atCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGAtt                                               >  1:126582/1‑122 (MQ=255)
                                                                                                                                     tGTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGt                                                                       <  2:771140/95‑1 (MQ=255)
                                                                                                                                      gTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGGCGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGt               <  3:424340/150‑1 (MQ=255)
                                                                                                                                      gTCGCCCTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGt               >  2:909665/1‑150 (MQ=255)
                                                                                                                                       tCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTg              >  4:170302/1‑150 (MQ=255)
                                                                                                                                           ccTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCttt          >  1:96284/1‑150 (MQ=255)
                                                                                                                                            cTTGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCATGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCtttt         <  3:348635/150‑1 (MQ=255)
                                                                                                                                             ttGTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGa                                                                 <  2:681569/93‑1 (MQ=255)
                                                                                                                                             ttGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTtc        >  1:938575/1‑150 (MQ=255)
                                                                                                                                               gTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTaaa                                                                             <  1:959668/79‑1 (MQ=255)
                                                                                                                                               gTCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTaaa                                                                             >  2:959668/1‑79 (MQ=255)
                                                                                                                                                tCAACAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCa     <  2:880056/150‑1 (MQ=255)
                                                                                                                                                tCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCa     <  3:477256/150‑1 (MQ=255)
                                                                                                                                                  aaaaGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTg                                                   >  4:562610/1‑102 (MQ=255)
                                                                                                                                                   aaaGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTtctatggt  >  4:396840/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTG  >  NC_000913_3_pae_tpiA/4112042‑4112338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑