Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,747 G→C intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,7470GC98.8% 584.1 / ‑2.7 168intergenic (+62/+784)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (93/73);  minor base T (1/1);  total (94/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 170 total aligned reads displayed.

TGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGT  >  NC_000913_3_pae_tpiA/4110600‑4110892
                                                                                                                                                   |                                                                                                                                                 
  cTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGccc                                                                                                                                               >  3:201947/1‑150 (MQ=255)
   tGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGcccc                                                                                                                                              >  4:34996/1‑150 (MQ=255)
    ggCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCccg                                                                                                                                             >  2:742929/1‑150 (MQ=255)
     gCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCccgc                                                                                                                                            >  3:131252/1‑150 (MQ=255)
        acacAACAAAATATAATGCCACTAAACCATGATTCAGCGGGGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGACGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCcgccgc                                                                                                                                         <  4:462641/150‑1 (MQ=255)
          acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                       >  1:774680/1‑150 (MQ=255)
            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAggccgc                                                                                                                                                 <  3:620635/138‑1 (MQ=255)
            aaCGAAATCTACTGACACTTAACCGTGCTTCAGGCGAGGGAAATCAGGATCATCAGTGTGAGAATGTGCGTTCAGGAGAAGAGTTAATGCAAATAAGCTCCACATATGAAAGCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGc                                                                                                                                     >  4:158311/1‑150 (MQ=255)
              cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCgg                                                                                                                                   >  4:320570/1‑150 (MQ=255)
              cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCgg                                                                                                                                   <  1:593180/150‑1 (MQ=255)
                 aaTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGttt                                                                                                                                <  3:341833/150‑1 (MQ=255)
                  aTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTc                                                                                                                               >  4:555669/1‑150 (MQ=255)
                      aCTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCa                                                                                                                           >  2:802747/1‑150 (MQ=255)
                       cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                          <  2:1005573/150‑1 (MQ=255)
                         gACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAtt                                                                                                                        <  3:236962/150‑1 (MQ=255)
                            gCTTAACCGTGCTTCAGCCGAGGACATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTa                                                                                                                     >  2:212643/1‑150 (MQ=255)
                             cTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTaa                                                                                                                    <  1:388478/150‑1 (MQ=255)
                              ttAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGATATTTTGCGTTAAGCCGAAGAGGTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCTCGCCGCGAGCGGTTTCGCCCATTCTCAg                                                                                                                   >  4:477492/1‑150 (MQ=255)
                              ttAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAg                                                                                                                   >  4:608407/1‑150 (MQ=255)
                                aaCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGACAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCAGCCGCGAGCGGTTTCGCCAATTCTAAGAt                                                                                                                 >  4:601362/1‑150 (MQ=255)
                                  ccGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAtt                                                                                                                        <  2:934370/141‑1 (MQ=255)
                                   cGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCtt                                                                                                              >  3:442299/1‑150 (MQ=255)
                                    gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTa                                                                                                             >  1:354043/1‑150 (MQ=255)
                                        ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGat                                                                                                         >  1:49641/1‑150 (MQ=255)
                                           aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAANATATg                                                                                                      >  3:171039/1‑150 (MQ=255)
                                            gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAGAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGCTCTTAAGATATgg                                                                                                     >  1:595120/1‑150 (MQ=255)
                                            gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCACCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                     <  2:483429/150‑1 (MQ=255)
                                              cGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGt                                                                                                   <  2:49641/150‑1 (MQ=255)
                                               gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATACGGCCCTTATAAGACTTAAGGACGCGCAGCGTCGAGCGGTTTCGCCAATTCTCAGATCTTAAGATATGGGTc                                                                                                  >  1:151474/1‑150 (MQ=255)
                                                  gAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGACGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCttt                                                                                               >  2:428588/1‑150 (MQ=255)
                                                    aaTTCAGTATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTg                                                                                             >  2:476226/1‑150 (MQ=255)
                                                    aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTg                                                                                             <  3:509927/150‑1 (MQ=255)
                                                      ttCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGcc                                                                                           >  4:33982/1‑150 (MQ=255)
                                                      ttCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATAAAAAACTGAACATGTCATATCAAACTCCTCTCCTCCTCGATCGTGGTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGcc                                                                                           >  1:876883/1‑150 (MQ=255)
                                                       tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCt                                                                                          >  2:484717/1‑150 (MQ=255)
                                                           gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCAc                                                                                      >  4:371231/1‑150 (MQ=255)
                                                              catcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGTCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGATCTTTTGCCTTCACGGc                                                                                   >  1:146429/1‑150 (MQ=255)
                                                                tcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTg                                                                                 >  1:124839/1‑150 (MQ=255)
                                                                  aGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGTCGCCCcgccgc                                                                                                                                         <  1:917709/92‑1 (MQ=255)
                                                                    tgtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCt                                                                             >  2:550496/1‑150 (MQ=255)
                                                                     gtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                            <  1:252414/150‑1 (MQ=255)
                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                           >  1:189577/1‑150 (MQ=255)
                                                                      tgAGATTTTGCGTTAAGCCTAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                           <  3:34996/150‑1 (MQ=255)
                                                                       gagaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTc                                                                                                                               <  4:537938/97‑1 (MQ=255)
                                                                       gagaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTc                                                                                                                               >  3:537938/1‑97 (MQ=255)
                                                                         gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                        <  3:555669/150‑1 (MQ=255)
                                                                           ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGc                                                                      <  4:201947/150‑1 (MQ=255)
                                                                           ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGc                                                                      <  1:856227/150‑1 (MQ=255)
                                                                             ttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCaa                                                                    <  2:983003/150‑1 (MQ=255)
                                                                                 gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                            <  1:707576/138‑1 (MQ=255)
                                                                                 gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCTGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                            >  2:707576/1‑138 (MQ=255)
                                                                                  ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTc                                                               >  3:331699/1‑150 (MQ=255)
                                                                                  ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTc                                                               <  1:948070/150‑1 (MQ=255)
                                                                                     aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcccc                                                            <  4:74101/150‑1 (MQ=255)
                                                                                              gTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                   <  2:909272/150‑1 (MQ=255)
                                                                                              gTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGa                                                   <  3:217455/150‑1 (MQ=255)
                                                                                               ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAg                                                  >  4:227643/1‑150 (MQ=255)
                                                                                                tAAGGAAAGTAAGTGACGGATATGAAATCCTGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACGCCCCTCGTGCCGAGt                                                 <  4:631377/150‑1 (MQ=255)
                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTccc                                                             >  3:316205/1‑137 (MQ=255)
                                                                                                 aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGtt                                                <  4:597737/150‑1 (MQ=255)
                                                                                                    gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                     >  2:252729/1‑94 (MQ=255)
                                                                                                     aaaGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAg                                            >  2:980392/1‑150 (MQ=255)
                                                                                                      aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTa                                                                                                             >  2:605037/1‑84 (MQ=255)
                                                                                                        gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAgcgc                                         >  2:883586/1‑150 (MQ=255)
                                                                                                        gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACGTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCGACACTCCCCTCGTGCCGCGTTTAAgcgc                                         >  4:253622/1‑150 (MQ=255)
                                                                                                          aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTGCACGGCTGCGCTTGCCAGCAACCCTCCCCTCGTGCCGAGTTTAAGCGCgg                                       <  2:91523/150‑1 (MQ=255)
                                                                                                             tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                    <  4:127108/150‑1 (MQ=255)
                                                                                                             ggCCGTATATGCTATCCGTCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGTTCTGTTGCCTTCACGGTTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                    <  2:151474/149‑1 (MQ=255)
                                                                                                               ccGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCGTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCGTCa                                  >  1:775341/1‑150 (MQ=255)
                                                                                                                cGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCaa                                 >  4:295540/1‑150 (MQ=255)
                                                                                                                  gATATGAAATCTGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACg                               >  2:134032/1‑150 (MQ=255)
                                                                                                                     atGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTcc                            <  1:822554/150‑1 (MQ=255)
                                                                                                                      tGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCct                           <  1:283642/150‑1 (MQ=255)
                                                                                                                      tGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCct                           <  3:370621/150‑1 (MQ=255)
                                                                                                                       gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAaca                                                                  >  1:641071/1‑110 (MQ=255)
                                                                                                                       gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                          >  3:593568/1‑150 (MQ=255)
                                                                                                                       gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                          >  1:484483/1‑150 (MQ=255)
                                                                                                                       gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGAGCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAaca                                                                  <  2:641071/110‑1 (MQ=255)
                                                                                                                        aaaTCCGGCAGCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCTGTTTCGCCAATTCTAAGATCTTAAGAGATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         >  4:537830/1‑150 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         >  3:400144/1‑150 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         <  2:774680/150‑1 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         >  1:239149/1‑150 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         <  3:390211/150‑1 (MQ=255)
                                                                                                                        aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTATAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                         >  1:605997/1‑150 (MQ=255)
                                                                                                                         aaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCtctc                        >  3:604800/1‑150 (MQ=255)
                                                                                                                           tCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCg                      <  3:292911/150‑1 (MQ=255)
                                                                                                                           tCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCg                      <  2:321360/150‑1 (MQ=255)
                                                                                                                             cGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                    <  4:442299/150‑1 (MQ=255)
                                                                                                                                    tGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGAGATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATa             <  1:40564/150‑1 (MQ=255)
                                                                                                                                     gTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGcc                                                                                           <  2:152930/71‑1 (MQ=255)
                                                                                                                                     gTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc            <  4:134225/150‑1 (MQ=255)
                                                                                                                                        aGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCg         >  1:250379/1‑150 (MQ=255)
                                                                                                                                          aCTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTAAAAGTCCTCTCCGCTCTCAATACCCGcc       >  1:740847/1‑150 (MQ=255)
                                                                                                                                           cTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGccc      >  1:679360/1‑150 (MQ=255)
                                                                                                                                            ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                     <  1:526379/54‑1 (MQ=255)
                                                                                                                                            ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcg                                                                               <  2:518368/76‑1 (MQ=255)
                                                                                                                                            ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcccc     <  2:18969/150‑1 (MQ=255)
                                                                                                                                            ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcccc     >  1:18969/1‑150 (MQ=255)
                                                                                                                                              aaGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAg   >  1:888261/1‑150 (MQ=255)
                                                                                                                                               aGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGt  <  1:319167/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGT  >  NC_000913_3_pae_tpiA/4110600‑4110892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: