Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,472,342 (A)8→9 coding (108/135 nt) pyrL ← pyrBI operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,472,3341.A88.0% 241.8 / 27.0 75coding (116/135 nt)pyrLpyrBI operon leader peptide
Reads supporting (aligned to +/- strand):  ref base . (6/3);  new base A (29/37);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATAC  >  NC_000913_3_pae_tpiA/4472187‑4472481
                                                                                                                                                     |                                                                                                                                                         
tgCTTCAACAGCTCTGGTTGCGGGGTTGGTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaa                                                                                                                                                         >  3:255423/1‑150 (MQ=255)
    tCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aa                                                                                                                                                  >  4:515210/1‑150 (MQ=255)
      aaCAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aaaa                                                                                                                                               >  4:464703/1‑150 (MQ=255)
         aGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aaaaagc                                                                                                                                            >  1:776377/1‑147 (MQ=255)
                     gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAg                                                                                                                               >  2:770667/1‑150 (MQ=255)
                     gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAg                                                                                                                               >  2:732232/1‑150 (MQ=255)
                     gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAg                                                                                                                               >  4:142908/1‑150 (MQ=255)
                     gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCCCGATTGAg                                                                                                                               >  4:364244/1‑150 (MQ=255)
                       gTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAGGGACATCGCGACTAAGGTCGGTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAACAAAAGACCCTCGATTGAgg                                                                                                                              >  1:205910/1‑150 (MQ=255)
                       gTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAggg                                                                                                                             >  1:138174/1‑150 (MQ=255)
                            cTTTCATGTGCGCCGGTGAGGCCGGCACCAGATTAAGGTCATCGCGACTAAGATGGTTTATGGAAATGATATGTTTCTGATATAGAGGATTAGTCATCTTTTATCTCCTGACGCAAGGAAAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTgg                                                                                                                        <  4:336579/150‑1 (MQ=255)
                             tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCCTCGCGAATAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGAGTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTggg                                                                                                                       >  1:22319/1‑150 (MQ=255)
                             tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTggg                                                                                                                       >  3:341646/1‑150 (MQ=255)
                               tCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCAATCGAGGGGCtttttt                                                                                                                     <  1:111248/150‑6 (MQ=255)
                                 aGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATg                                                                                                                   >  2:783159/1‑150 (MQ=255)
                                  gTTTCGGCGCGGGCGCCAGCAACAGATGAAGGGCATGGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGAAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                                                  <  2:535159/150‑1 (MQ=255)
                                  gTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCATGGACAAAAA‑‑AAAAGCCCCTCGATTGAGGTGCTGGGAATgg                                                                                                                  <  2:535117/150‑1 (MQ=255)
                                  gTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGACTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                                                  <  2:624904/150‑1 (MQ=255)
                                   tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATggg                                                                                                                 >  2:231155/1‑150 (MQ=255)
                                     tCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AACAAGCCCCTCGATGGAGGGGCTCGGactccgtc                                                                                                               >  1:627430/1‑143 (MQ=255)
                                       gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGACTGAGGGGGTGGGATTGGTTGAt                                                                                                             >  2:34177/1‑150 (MQ=255)
                                        ccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATc                                                                                                            <  3:239692/150‑1 (MQ=255)
                                           cTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGAAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAAc                                                                                                         <  3:518699/150‑1 (MQ=255)
                                            tGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAAca                                                                                                        <  2:188902/150‑2 (MQ=255)
                                             gTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACgg                                                                                                       <  1:726433/150‑1 (MQ=255)
                                                gCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaa                                                                                                    <  1:246331/150‑1 (MQ=255)
                                                 ccAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGgaag                                                                                                   <  3:42392/150‑1 (MQ=255)
                                                     cACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCt                                                                                                                                       <  1:775887/110‑1 (MQ=255)
                                                     cACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCt                                                                                                                                       >  2:775887/1‑110 (MQ=255)
                                                        cAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACg                                                                                            >  2:815685/1‑150 (MQ=255)
                                                           aTTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCa                                                                                         <  1:321168/150‑1 (MQ=255)
                                                           aTTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCa                                                                                         <  3:353620/150‑1 (MQ=255)
                                                           aTTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCa                                                                                         <  3:26122/150‑1 (MQ=255)
                                                                 gTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGc                                                                                  >  1:106577/1‑150 (MQ=255)
                                                                     tCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTc                                                                               >  3:91864/1‑150 (MQ=255)
                                                                     tCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aaaaa                                                                                                                                              <  3:86457/88‑2 (MQ=255)
                                                                     tCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aaaaa                                                                                                                                              >  4:86457/1‑87 (MQ=255)
                                                                      cgcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aaaaa                                                                                                                                              >  4:313026/1‑86 (MQ=255)
                                                                      cgcgACTAAGGTCGTTTAGGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCaaaa‑‑aaaaa                                                                                                                                              <  3:313026/87‑2 (MQ=255)
                                                                       gcgACTAAGGGGGTTTATGGAAATGATATGTTACTGAGAAAACTGATTAGCCATCTTTTATCACCTGACGAATGAGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGtggt                                                                             <  2:321469/150‑4 (MQ=255)
                                                                          aCTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttttt                                                                          <  3:464703/150‑1 (MQ=255)
                                                                            tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGGCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                                                        <  1:465658/150‑1 (MQ=255)
                                                                            tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                                                        <  1:478125/150‑1 (MQ=255)
                                                                            tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                                                        >  3:74827/1‑150 (MQ=255)
                                                                            tAAGGACGTTTATGGAAATTATATGTTTCTGATATAGCGGATTATCCATCTTTTATCTCCTAACAACTGGGAAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGTTCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTc                                                                        <  4:15771/150‑1 (MQ=255)
                                                                               ggTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AAAACGCCACTCGATAGACGAGCAACGCACGGCAAAACAACCGGAAGAAAAAAGGCAGGCAAGCGTCTTTTTTCAGa                                                                     >  3:47508/1‑150 (MQ=255)
                                                                                gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAc                                                                    >  4:20660/1‑150 (MQ=255)
                                                                                   gTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                                                    >  2:341246/1‑131 (MQ=255)
                                                                                   gTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                                                    <  1:341246/131‑1 (MQ=255)
                                                                                    tttggggAAATGATATGTTTCTGGTATAGCGGATGAGCCATCTTGTATCGCCTGACGGCTGGGCAAAGA‑‑AAGAGCTCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCGGGCCAGTGGCTTTTTTCAGACGCgg                                                                >  4:445603/6‑150 (MQ=255)
                                                                                       aTGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTaa                                                             <  4:91864/150‑1 (MQ=255)
                                                                                        tGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAg                                                            >  2:295682/1‑150 (MQ=255)
                                                                                        tGGAAATGATATGGTTCTGATATAGCGGATTAACCATCTTTTAACTCCGGACGCCTTGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGAAAATGGGAGAACAACGCGTATAGATTCTTCAGGCCAGCGTGTTTTCTGAGACGGGGTAAg                                                            <  2:22319/150‑1 (MQ=255)
                                                                                            agtGATATGGGTCTGATAGAGCGGATTAGCCATGTTTGATTTCCGGACGAAAGAAAAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaa                                                        <  2:694617/148‑1 (MQ=255)
                                                                                                 tatGTTTGTGATATAGCGGATTAGCCATCTTTTATCGCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCATCGATTGAGGGGCTGGGAATGGGTGAAAAAAAAAAAAAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt                                                   <  2:673960/150‑1 (MQ=255)
                                                                                                 tatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt                                                   <  3:20660/150‑1 (MQ=255)
                                                                                                  atGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc                                                  <  1:815685/150‑1 (MQ=255)
                                                                                                   tGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                                                 <  1:770667/150‑1 (MQ=255)
                                                                                                     tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AAAAACCCCGTTGATGGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa                                               <  3:9272/150‑1 (MQ=255)
                                                                                                     tttCTAATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGACATTCTATTGAGGGGCTGGGAATGGGTGATTAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa                                               <  3:9323/150‑1 (MQ=255)
                                                                                                       tCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTCAGATAAAATGTCGAaca                                             >  1:37043/1‑150 (MQ=255)
                                                                                                         tGATATAGCGGAGTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca                                           <  3:469353/150‑1 (MQ=255)
                                                                                                              taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACCAAATGTCGAACACACTGaa                                      >  2:26915/1‑150 (MQ=255)
                                                                                                               aGCGGATTAGACATCTTTTATCTACTGACGCCTGGGAAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc                                     <  2:365153/150‑1 (MQ=255)
                                                                                                                gCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa                                   >  1:731727/1‑150 (MQ=255)
                                                                                                                   gATTAGCCATCTTTTATTTTTTTTTGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTTAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa                                 >  4:328797/1‑150 (MQ=255)
                                                                                                                   gATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATa                                 <  3:142908/150‑1 (MQ=255)
                                                                                                                        gCCATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGAATGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAACCCGGCAGGTCAGCGACTTTTTTCAGACGCGGTAAGACAAAAGGTGGAACACACTGAACCACACAgcc                            >  4:292445/1‑150 (MQ=255)
                                                                                                                          cATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAACAAGGCCGGCCAGCGTCTTTTTTCAGACGAGGTAAGAAAAACAGTCGACTAAACTGAACAATACAtcata                          >  1:376665/1‑147 (MQ=255)
                                                                                                                          cATCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcctc                          >  2:727050/1‑150 (MQ=255)
                                                                                                                            tCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGt                                                                                                                >  4:429610/1‑62 (MQ=255)
                                                                                                                            tCTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGt                                                                                                                <  3:429610/62‑1 (MQ=255)
                                                                                                                             cTTTTATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCg                       <  1:732232/150‑1 (MQ=255)
                                                                                                                              ttttATCTCCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGTATGGGTGATCAGCGGGACGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCgg                      <  1:34177/150‑1 (MQ=255)
                                                                                                                                     tcCTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTg               >  1:483077/1‑150 (MQ=255)
                                                                                                                                       cTGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACGGAACCATACATCCTCCCGGCAAATTGTc             >  3:465984/1‑150 (MQ=255)
                                                                                                                                        tGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTcc            <  1:727050/150‑1 (MQ=255)
                                                                                                                                        tGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTcc            >  1:326047/1‑150 (MQ=255)
                                                                                                                                        tGACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTcc            >  1:296122/1‑150 (MQ=255)
                                                                                                                                         gACGCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCg           <  3:256008/150‑1 (MQ=255)
                                                                                                                                            gCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa        >  4:197337/1‑150 (MQ=255)
                                                                                                                                            gCCTGGGCAAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa        <  2:475580/150‑1 (MQ=255)
                                                                                                                                                 ggCAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATAc  <  2:106577/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
TGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAA‑‑AAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATAC  >  NC_000913_3_pae_tpiA/4472187‑4472481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: