| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 314,816 | (G)5→6 | pseudogene (460/872 nt) | eaeH → | pseudogene, attaching and effacing protein homology;factor; Not classified |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 314,811 | 1 | . | G | 98.2% | 190.1 / ‑3.2 | 54 | pseudogene (455/872 nt) | eaeH | pseudogene, attaching and effacing protein homology;factor; Not classified |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (26/27); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑GGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCATTGGTGTT > NC_000913_3_pae_tpiA/314663‑314953 | aaagctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑g > 2:429683/1‑150 (MQ=255) aagctaaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAGTGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑gg < 2:554830/150‑1 (MQ=255) aaCCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑ggggggc > 2:40159/1‑148 (MQ=255) aGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATa < 1:559282/150‑1 (MQ=255) ggAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATAc < 3:506998/150‑1 (MQ=255) aTACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCg < 3:184553/150‑1 (MQ=255) aGGATTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATTTTGTTCACTCAGGGGGGCAATACATCGTACa < 4:514304/150‑1 (MQ=255) gAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGa < 3:302529/150‑1 (MQ=255) cTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCg < 4:420428/150‑1 (MQ=255) atGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCa < 4:98832/146‑1 (MQ=255) atGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCa > 3:98832/1‑146 (MQ=255) ggTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCaaa < 1:362216/150‑1 (MQ=255) ggTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCaaa < 1:779126/150‑1 (MQ=255) cgcgcgTCAAACTGGATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCGGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTGA‑GGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGtt < 2:544557/150‑1 (MQ=255) cgcgcgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGt < 4:275115/150‑1 (MQ=255) cgcgcgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAAGGCTTTATCCGATTTATGATACGACGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACGGACGATGGTACTCAGTTAAATATTGGt > 2:415230/1‑150 (MQ=255) gcgcgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGtt < 1:41085/150‑1 (MQ=255) gcgcgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGtt > 2:564478/1‑150 (MQ=255) cgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATc < 4:249194/126‑1 (MQ=255) cgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATc > 3:249194/1‑126 (MQ=255) cgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtg > 1:108138/1‑150 (MQ=255) cgTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtg < 3:504437/150‑1 (MQ=255) tCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtggc > 3:509216/1‑150 (MQ=255) cAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtggct > 1:291948/1‑150 (MQ=255) aaaCTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtggctg < 1:691244/150‑1 (MQ=255) aCTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtggctggc > 1:341419/1‑150 (MQ=255) gTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑ggg > 2:744412/1‑89 (MQ=255) gTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑ggg < 1:744412/89‑1 (MQ=255) aTAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCa < 1:427006/150‑1 (MQ=255) aTAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCa > 2:361003/1‑150 (MQ=255) aGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGaa > 1:769975/1‑150 (MQ=255) cGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTgg > 2:191323/1‑150 (MQ=255) tGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATg > 2:166199/1‑150 (MQ=255) aaGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGc > 3:315444/1‑150 (MQ=255) aGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCg < 3:105206/150‑1 (MQ=255) ggATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTAAGGAAATGACTGGATGGCgg > 3:373872/1‑150 (MQ=255) gATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCggg < 2:275935/150‑1 (MQ=255) ttcttcGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCggggg < 2:414072/150‑1 (MQ=255) tcttcGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGt < 2:499509/150‑1 (MQ=255) tcttcGCTGGAAATGCATTATCCGATTTATGATACGCCGACAAATCTGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGt < 1:463056/150‑1 (MQ=255) cttcGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTg > 2:792508/1‑150 (MQ=255) cGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAAt < 1:40159/150‑1 (MQ=255) gCTGGAAATGCTTCATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTCTTCAGGAAATGACTGGATGGCTGGGGTGAATa > 2:661330/1‑150 (MQ=255) aaTGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTa < 3:508001/150‑1 (MQ=255) tttATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGAt > 2:83398/1‑150 (MQ=255) ttATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACATATTTTCGTTTTCTTTTAAATATTGGTTTTGGCTGGCGTCATTTTTTAGTAAATGACTGGATGGCGGGGGTGAATTCTTTTATCGATc > 4:452206/1‑150 (MQ=255) tCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATg < 4:271954/150‑1 (MQ=255) agATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGAt < 2:137128/149‑1 (MQ=255) aTGATACGCCGACAAATATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATcc > 2:675016/1‑150 (MQ=255) aTACGCCGACAAATATGTTGTTCACTCAGGGGGGCACTACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGt > 4:415746/1‑150 (MQ=255) aaaTATGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATAccc < 4:235061/150‑1 (MQ=255) tatGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCa < 1:792508/150‑1 (MQ=255) atGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAt > 4:435687/1‑150 (MQ=255) atGTTGTTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAt > 4:497908/1‑150 (MQ=255) tgttgtTCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAtt > 3:341291/1‑150 (MQ=255) gttgttCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAttg > 1:806762/1‑150 (MQ=255) gttCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAttggtg > 1:603501/1‑150 (MQ=255) ttCACTCAGGGGGGCAATACATCGTCCAGACGATCGTACTCAGTCAAATATTGGTCATGGCAGGCGTCATTTTTAAGGAAATGTCTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAttggtgt < 1:415230/150‑1 (MQ=255) tCACTCAGGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCAttggtgtt > 2:127908/1‑150 (MQ=255) | AAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCA‑GGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTGGCGTCATTTTTCAGGAAATGACTGGATGGCGGGGGTGAATACTTTTATCGATCATGATTTATCCCGTAGTCATACCCGCATTGGTGTT > NC_000913_3_pae_tpiA/314663‑314953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |