BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,295,976	0	C	T	34.9%	121.0 / 100.1	106	intergenic (+266/+376)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand): ref base C (38/31); new base T (22/15); total (60/46)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC  >  NC_000913_3_pae_tpiA/4295828‑4296113

gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACg                                                                                                                                          >  3:46645/1‑150 (MQ=32)

 tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGc                                                                                                                                         >  3:235226/1‑150 (MQ=17)

   ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCt                                                                                                                                       <  1:332390/150‑1 (MQ=32)

   ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGAGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCt                                                                                                                                       <  4:222292/150‑1 (MQ=17)

    gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTg                                                                                                                                      >  3:425599/1‑150 (MQ=17)

     aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGa                                                                                                                                     >  3:308163/1‑150 (MQ=32)

      tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGAt                                                                                                                                    >  1:348626/1‑150 (MQ=17)

       aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATg                                                                                                                                   >  4:250439/1‑150 (MQ=17)

         ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCg                                                                                                                                 >  1:455264/1‑150 (MQ=32)

          gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGa                                                                                                                                >  3:228613/1‑150 (MQ=17)

          gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGa                                                                                                                                >  1:84977/1‑150 (MQ=17)

            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACg                                                                                                                              >  3:283724/1‑150 (MQ=17)

               tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCtt                                                                                                                           <  4:308163/150‑1 (MQ=32)

                 tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGc                                                                                                                         <  1:87677/150‑1 (MQ=21)

                 tGCCGCAGCCGACATCATCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGc                                                                                                                         <  1:482674/150‑1 (MQ=21)

                  gCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGcc                                                                                                                        <  2:652572/150‑1 (MQ=25)

                  gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTga                                                                                                                         >  4:65000/1‑149 (MQ=255)

                   ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCcg                                                                                                                       >  3:238069/1‑150 (MQ=21)

                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCcgc                                                                                                                      >  3:439459/1‑150 (MQ=21)

                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCcgc                                                                                                                      <  2:609033/150‑1 (MQ=21)

                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCcgc                                                                                                                      >  1:392105/1‑150 (MQ=21)

                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCcgc                                                                                                                      >  1:101163/1‑150 (MQ=25)

                    cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCcgc                                                                                                                      <  4:46645/150‑1 (MQ=37)

                     gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCcgcg                                                                                                                     <  4:128180/150‑1 (MQ=21)

                       aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGAt                                                                                                                                    <  1:523738/133‑1 (MQ=17)

                       aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGAt                                                                                                                                    >  2:523738/1‑133 (MQ=17)

                          cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCagcgggcga                                                                                                                <  1:815167/150‑6 (MQ=255)

                          cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                >  2:697232/1‑150 (MQ=21)

                          cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                >  3:83710/1‑150 (MQ=39)

                           gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                               <  3:109072/150‑1 (MQ=39)

                           gACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                               >  4:432436/1‑150 (MQ=17)

                            aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                              <  4:478629/150‑1 (MQ=21)

                              aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                            <  4:492633/150‑1 (MQ=21)

                              aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTACCGCGTCTTATCAg                                                                                                            >  1:725612/1‑150 (MQ=17)

                               tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                           >  3:165477/1‑150 (MQ=25)

                                cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                          >  1:588328/1‑150 (MQ=21)

                                cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                          >  2:421100/1‑150 (MQ=37)

                                cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGc                                                                                                          >  4:197330/1‑150 (MQ=17)

                                       tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                                                                   >  1:737917/1‑146 (MQ=9)

                                           gCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGa                                                                                                                                     <  3:523514/112‑1 (MQ=17)

                                           gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAccttaacc                                                                                               >  2:758059/1‑143 (MQ=12)

                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                   <  2:395438/128‑1 (MQ=25)

                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                   >  1:395438/1‑128 (MQ=25)

                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                           >  1:565820/1‑136 (MQ=25)

                                             gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                           <  2:565820/136‑1 (MQ=21)

                                                  ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCt                                                                                                                            <  3:346692/114‑1 (MQ=18)

                                                  ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCt                                                                                                                            >  4:346692/1‑114 (MQ=18)

                                                    aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                    <  1:50497/120‑1 (MQ=40)

                                                    aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                    >  2:50497/1‑120 (MQ=40)

                                                     ccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGc                                                                                                                         <  3:316301/113‑1 (MQ=18)

                                                         ggcTTATCAGCCCGACGCCAGACAGCGCAATAGCATGATTGAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                 <  3:99857/148‑1 (MQ=11)

                                                         gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                 <  1:112941/150‑1 (MQ=39)

                                                            ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                              >  1:740401/1‑150 (MQ=39)

                                                              aTCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                                                            <  1:742025/150‑1 (MQ=38)

                                                              aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGc                                                                                                                             <  3:492938/101‑1 (MQ=33)

                                                                     cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                          >  2:641370/1‑113 (MQ=255)

                                                                     cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                          <  1:641370/113‑1 (MQ=40)

                                                                            aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                                                              <  4:83710/150‑1 (MQ=37)

                                                                             gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCtt                                                                                                                           >  3:109290/1‑88 (MQ=35)

                                                                              aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                          >  1:799533/1‑136 (MQ=40)

                                                                               cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                           <  1:678102/150‑1 (MQ=38)

                                                                                  cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGAt                                                                                                                                    >  4:327835/1‑74 (MQ=34)

                                                                                        aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTg                                                                                                                          >  2:707763/1‑78 (MQ=25)

                                                                                        aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTg                                                                                                                          <  1:707763/78‑1 (MQ=255)

                                                                                         gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                            >  2:713552/1‑91 (MQ=255)

                                                                                         gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                            <  1:713552/91‑1 (MQ=255)

                                                                                          ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                >  4:378463/1‑150 (MQ=37)

                                                                                                 tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                         <  1:421100/150‑1 (MQ=37)

                                                                                                   gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                   <  3:301623/138‑1 (MQ=38)

                                                                                                    cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCTCGTGACTCTGCAGGTCGGATAATGCCATTCTGCTGCa                                      >  2:103631/1‑150 (MQ=11)

                                                                                                      tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc                                                                                                    >  4:474822/1‑86 (MQ=255)

                                                                                                      tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc                                                                                                    <  3:474822/86‑1 (MQ=255)

                                                                                                      tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGCTTTAGCGTCATTTTGTAGGTCGGATAATTCGTTTACGCCCCATc                                    >  2:551337/1‑150 (MQ=11)

                                                                                                           ttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa                               <  1:669437/150‑1 (MQ=38)

                                                                                                            tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCg                                                                                                                                 <  1:502254/51‑1 (MQ=255)

                                                                                                            tGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCg                                                                                                                                 >  2:502254/1‑51 (MQ=38)

                                                                                                             gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                             >  1:74160/1‑150 (MQ=39)

                                                                                                              tAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCa                            <  1:465289/150‑1 (MQ=37)

                                                                                                                ggTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCAGAGTGCTCATGTAGCTCCTATAAGGCGTTTATGCCGCATCCGACATCAAc                          <  2:455264/150‑1 (MQ=11)

                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGcc                       <  3:378463/150‑1 (MQ=39)

                                                                                                                    ggATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt                      >  2:709671/1‑150 (MQ=255)

                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGGCTCTTGCCGCGTCTTATCATGCCTACCCCAAACAGCGCAATAGCCTGATTTATCGTCATTTTGTAGCTCGGATAAGGCCTTTATGCCTCATCCGACACCAACGCCTg                     >  2:624109/1‑150 (MQ=11)

                                                                                                                       tAAGGCGTTTACGCCGCATCCGACA‑TCAATGCCTGATGCGACGCTTGc                                                                                                                         <  2:408056/48‑1 (MQ=255)

                                                                                                                       tAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGc                                                                                                                         >  1:408056/1‑48 (MQ=255)

                                                                                                                          ggCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtt                                                                    >  2:822470/1‑98 (MQ=255)

                                                                                                                          ggCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                >  3:264379/1‑150 (MQ=39)

                                                                                                                           gCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg                                                                       >  2:473716/1‑94 (MQ=255)

                                                                                                                             gTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGcc                                                                                                                        >  2:475473/1‑43 (MQ=255)

                                                                                                                             gTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGcc                                                                                                                        <  1:475473/43‑1 (MQ=255)

                                                                                                                             gTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATgagaga             >  2:603362/1‑148 (MQ=255)

                                                                                                                              tttACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                                                              >  1:56810/1‑100 (MQ=255)

                                                                                                                              tttACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                                                              <  2:56810/100‑1 (MQ=255)

                                                                                                                                    ccgcATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACcg      >  1:338822/1‑150 (MQ=25)

                                                                                                                                    ccgcATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAAGCGACGCTTAAcg      >  1:338834/1‑150 (MQ=255)

                                                                                                                                        aTCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc  <  3:432436/150‑1 (MQ=35)

                                                                                                                                                 tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAtgc                                                     >  4:327463/1‑89 (MQ=25)

                                                                                                                                                 tCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAtgc                                                     <  3:327463/91‑3 (MQ=25)

                                                                                                                                                   aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                <  1:447028/78‑1 (MQ=34)

                                                                                                                                                     cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAc                                <  2:1885/108‑1 (MQ=11)

                                                                                                                                                     cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAc                                >  1:1885/1‑108 (MQ=11)

GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACA‑TCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTC  >  NC_000913_3_pae_tpiA/4295828‑4296113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`