Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 2,670,017 G→A G329D (GGC→GAC)  hcaE → 3‑phenylpropionate dioxygenase, large (alpha) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,670,0170GA98.7% 256.9 / NA 75G329D (GGC→GAC) hcaE3‑phenylpropionate dioxygenase, large (alpha) subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/36);  minor base C (0/1);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGG  >  NC_000913_3_pae_tpiA/2669879‑2670156
                                                                                                                                          |                                                                                                                                           
aTTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAg                                                                                                                                  >  2:714372/1‑150 (MQ=255)
   aCCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGTTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTg                                                                                                                               >  4:492818/1‑150 (MQ=255)
     cgcgAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGaa                                                                                                                             <  1:250915/150‑1 (MQ=255)
     cgcgAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGaa                                                                                                                             >  3:470803/1‑150 (MQ=255)
        gAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAgtg                                                                                                                          <  2:400943/150‑1 (MQ=255)
                tGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGtt                                                                                                                  >  4:54152/1‑150 (MQ=255)
                tGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGtt                                                                                                                  <  2:568117/150‑1 (MQ=255)
                 gCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTc                                                                                                                 >  2:268013/1‑150 (MQ=255)
                  ccGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCt                                                                                                                <  3:412023/150‑1 (MQ=255)
                   cGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTg                                                                                                               >  1:725649/1‑150 (MQ=255)
                    gAAGCAGAACAACGTTTAGGTGAAGTTCGCACCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTCCCACGCTCCGCGTCTGGCATCCGCGCGACCCTGATTACGTTGAAGTGTGGGCGTTCTGt                                                                                                              <  2:78614/150‑1 (MQ=255)
                     aaGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTa                                                                                                             <  2:1722/150‑1 (MQ=255)
                       gCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTAtt                                                                                                           <  2:725649/150‑1 (MQ=255)
                        cAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTa                                                                                                          <  2:536656/150‑1 (MQ=255)
                              aacGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACa                                                                                                    >  4:168968/1‑150 (MQ=255)
                              aacGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACa                                                                                                    >  4:334175/1‑150 (MQ=255)
                                ggTTTAGGTGAAGTTCGCGCCCTGCGGCTGGCGGGTCATAACAATGTTTTCCCCACGCTTGCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGGGGGCGTTCTGTATTACTGACaaa                                                                                                  <  2:133387/149‑1 (MQ=255)
                                     aGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAgccgc                                                                                             <  1:353150/150‑1 (MQ=255)
                                       gTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGAcc                                                                                                                                           <  4:484848/102‑1 (MQ=255)
                                       gTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTgg                                                                                                                       >  2:228797/1‑122 (MQ=255)
                                       gTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTgg                                                                                                                       <  1:228797/122‑1 (MQ=255)
                                        tGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTc                                                                                          <  1:298178/150‑1 (MQ=255)
                                        tGAAGTTCGCGCCCCGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTc                                                                                          <  3:424161/150‑1 (MQ=255)
                                          aaGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCg                                                                                        >  4:513034/1‑150 (MQ=255)
                                             ttCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATg                                                                                     >  3:315952/1‑150 (MQ=255)
                                             ttCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATg                                                                                     >  3:272977/1‑150 (MQ=255)
                                               cgcgCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGaa                                                                                   >  4:55492/1‑150 (MQ=255)
                                                 cgcCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGt                                                                                 <  1:462552/150‑1 (MQ=255)
                                                     cTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTaaa                                                                             <  1:393632/150‑1 (MQ=255)
                                                           cTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCt                                                                       >  1:417501/1‑150 (MQ=255)
                                                             ggCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCttt                                                                     <  1:343189/150‑1 (MQ=255)
                                                                 ggTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGaa                                                                 <  3:200995/150‑1 (MQ=255)
                                                                      tAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAg                                                            >  3:25894/1‑150 (MQ=255)
                                                                       aaCAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGAt                                                                                      <  4:419881/123‑1 (MQ=255)
                                                                       aaCAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGAt                                                                                      >  3:419881/1‑123 (MQ=255)
                                                                            tatTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCAc                                                      <  4:315952/150‑1 (MQ=255)
                                                                              ttttCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTc                                                    >  3:471855/1‑150 (MQ=255)
                                                                              ttttCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTc                                                    <  4:297413/150‑1 (MQ=255)
                                                                                ttCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGt                                                  >  1:827162/1‑150 (MQ=255)
                                                                                    ccACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCtt                                              >  1:343395/1‑150 (MQ=255)
                                                                                    ccACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCtt                                              >  2:710987/1‑150 (MQ=255)
                                                                                     cACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCttt                                             >  2:809073/1‑150 (MQ=255)
                                                                                       cGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTg                                           <  4:286301/150‑1 (MQ=255)
                                                                                       cGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTg                                           <  1:189135/150‑1 (MQ=255)
                                                                                       cGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTg                                           <  3:334175/150‑1 (MQ=255)
                                                                                          tttCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTc                                        >  3:430629/1‑150 (MQ=255)
                                                                                              aTGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGCCCCTGATCAAGGTGAAGTGTGGGCGTCCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTATAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCctgc                                    <  2:684967/150‑1 (MQ=255)
                                                                                                ggCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCctgctg                                  <  3:513034/150‑1 (MQ=255)
                                                                                                 gCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTgg                                 >  1:231011/1‑150 (MQ=255)
                                                                                                  cTAAACGGCACTGCCATACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGt                                >  3:414919/1‑150 (MQ=255)
                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               >  2:700417/1‑150 (MQ=255)
                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               <  4:272977/150‑1 (MQ=255)
                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               >  4:114863/1‑150 (MQ=255)
                                                                                                   tAAACGGCACTGCCACACTCCGCGTCTGCCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGtt                               <  4:470803/150‑1 (MQ=255)
                                                                                                        ggCACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGATTTtctc                          >  1:667928/1‑150 (MQ=255)
                                                                                                          cACTGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGa                        <  4:25894/150‑1 (MQ=255)
                                                                                                             tGCCACACTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCa                     >  1:54851/1‑150 (MQ=255)
                                                                                                                cacaCTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  <  2:273856/150‑1 (MQ=255)
                                                                                                                cacaCTCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGATCTGTATTACTGACAAAACCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  >  4:495940/1‑150 (MQ=255)
                                                                                                                     tCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGaa                                                                                   <  2:175551/80‑1 (MQ=255)
                                                                                                                     tCCGCGTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGaa                                                                                   >  1:175551/1‑80 (MQ=255)
                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGt                                                                                 <  4:111016/79‑1 (MQ=255)
                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGt                                                                                 >  3:111016/1‑79 (MQ=255)
                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCgg          >  3:453947/1‑150 (MQ=255)
                                                                                                                        gcgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCgg          >  3:453977/1‑150 (MQ=255)
                                                                                                                         cgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGga         <  3:54152/150‑1 (MQ=255)
                                                                                                                         cgTCTGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGga         <  3:492818/150‑1 (MQ=255)
                                                                                                                             tGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  >  2:818543/1‑137 (MQ=255)
                                                                                                                             tGGCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGa                  <  1:818543/137‑1 (MQ=255)
                                                                                                                              ggCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACt    >  3:469695/1‑150 (MQ=255)
                                                                                                                              ggCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACt    >  1:809327/1‑150 (MQ=255)
                                                                                                                               gCATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTg   <  4:39384/150‑1 (MQ=255)
                                                                                                                                cATCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTgg  >  2:411587/1‑150 (MQ=255)
                                                                                                                                  tCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAgccgcc                                                                                            >  1:746550/1‑58 (MQ=255)
                                                                                                                                  tCCGCGCGACCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAgccgcc                                                                                            <  2:746550/58‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATTACCGCGAAACCTATGCCGAAGCAGAACAACGTTTAGGTGAAGTTCGCGCCCTGCGCCTGGCGGGTCATAACAATATTTTCCCCACGCTTTCATGGCTAAACGGCACTGCCACACTCCGCGTCTGGCATCCGCGCGGCCCTGATCAAGTTGAAGTGTGGGCGTTCTGTATTACTGACAAAGCCGCCTCCGATGAAGTTAAAGCCGCTTTTGAAAACAGCGCCACTCGTGCTTTTGGTCCTGCTGGTTTTCTCGAGCAGGATGACTCGGAGAACTGG  >  NC_000913_3_pae_tpiA/2669879‑2670156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑