Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 272,881 C→T pseudogene (35/1108 nt) ykfC → CP4‑6 prophage; conserved protein;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA272,8810CT98.6% 246.2 / ‑4.3 70pseudogene (35/1108 nt)ykfCCP4‑6 prophage; conserved protein;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/31);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

TGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAAT  >  NC_000913_3_pae_tpiA/272736‑273025
                                                                                                                                                 |                                                                                                                                                
tggcGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGTATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATgg                                                                                                                                              >  2:51728/1‑150 (MQ=255)
  gcGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGc                                                                                                                                            <  2:92553/150‑1 (MQ=255)
  gcGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGc                                                                                                                                            >  3:323342/1‑150 (MQ=255)
   cGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCa                                                                                                                                           <  1:515591/150‑1 (MQ=255)
        cTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCAc                                                                                                                                      >  1:186556/1‑150 (MQ=255)
                gtagtCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGt                                                                                                                              >  1:734318/1‑150 (MQ=255)
                     ccGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTa                                                                                                                         <  4:256048/150‑1 (MQ=255)
                      cgcgATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTAc                                                                                                                        >  1:3820/1‑150 (MQ=255)
                       gcgATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACg                                                                                                                       <  1:122885/150‑1 (MQ=255)
                               gAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATTATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAAc                                                                                                               <  2:541058/150‑1 (MQ=255)
                               gAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAAc                                                                                                               >  2:670198/1‑150 (MQ=255)
                                      cATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGgctgct                                                                                                        <  3:491266/150‑1 (MQ=255)
                                      cATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGgctgc                                                                                                         <  2:713786/149‑1 (MQ=255)
                                      cATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGgctgc                                                                                                         >  1:713786/1‑149 (MQ=255)
                                      cATTACATGGCGAAGGGAAGCAATTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGgctgct                                                                                                        >  1:278147/1‑150 (MQ=255)
                                       aTTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGctgctg                                                                                                       >  2:253286/1‑150 (MQ=255)
                                           cATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTc                                                                                                   >  3:228396/1‑150 (MQ=255)
                                            aTGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGCTCCGTCCCTACGGATTCAACGGCTGCTGCGTCt                                                                                                  <  2:431414/150‑1 (MQ=255)
                                            aTGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCt                                                                                                  <  3:351116/150‑1 (MQ=255)
                                               gCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGAt                                                                                               >  2:338658/1‑150 (MQ=255)
                                                 gAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTc                                                                                                                  >  2:403509/1‑129 (MQ=255)
                                                 gAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTc                                                                                                                  <  1:403509/129‑1 (MQ=255)
                                                 gAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATaa                                                                                             <  1:670198/150‑1 (MQ=255)
                                                  aaGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAac                                                                                            <  1:815856/150‑1 (MQ=255)
                                                         gCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCa                                                                                     >  1:287223/1‑150 (MQ=255)
                                                           aGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGa                                                                                   >  4:241642/1‑150 (MQ=255)
                                                                gAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAAtggc                                                                              <  2:186556/150‑1 (MQ=255)
                                                                      gtTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGgctggctg                                                                        >  4:323622/1‑150 (MQ=255)
                                                                       tttGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGctgctg                                                                                                       >  3:246077/1‑118 (MQ=255)
                                                                       tttGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGctgctg                                                                                                       <  4:246077/118‑1 (MQ=255)
                                                                          gCGACGTGAAGTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTCGCCATATGGGCAGCCACCGAACCGTCCCAACAGATTCAACGGCTGCTGCGTCTGATACCACAACCAGACTGGCTGGCCGAAgc                                                                    >  2:80734/1‑150 (MQ=255)
                                                                               gTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAg                                                                                                                                          >  2:572916/1‑75 (MQ=255)
                                                                               gTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAg                                                                                                                                          <  1:572916/75‑1 (MQ=255)
                                                                               gTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAACGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCTTACTGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGgcgc                                                               <  2:172695/150‑1 (MQ=255)
                                                                                 gAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCAATCCGTCCCTACGGATTCAACGgctg                                                                                                          <  3:186395/105‑1 (MQ=255)
                                                                                 gAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCAATCCGTCCCTACGGATTCAACGgctg                                                                                                          >  4:186395/1‑105 (MQ=255)
                                                                                  aaTTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATCCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGa                                                            <  1:573964/150‑1 (MQ=255)
                                                                                   aTTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGAt                                                           >  1:23372/1‑150 (MQ=255)
                                                                                    ttAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATc                                                          >  2:512759/1‑150 (MQ=255)
                                                                                       aCTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACg                                                       >  2:67352/1‑150 (MQ=255)
                                                                                           aCCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCttt                                                   >  2:796518/1‑150 (MQ=255)
                                                                                           aCCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCttt                                                   >  3:327188/1‑150 (MQ=255)
                                                                                                aCGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAgcg                                                                   <  2:667974/129‑1 (MQ=255)
                                                                                                aCGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAgcg                                                                   >  1:667974/1‑129 (MQ=255)
                                                                                                       gAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAggggg                                       <  4:359943/150‑1 (MQ=255)
                                                                                                          gACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGccc                                    >  3:440867/1‑150 (MQ=255)
                                                                                                            ccTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCAt                                  <  3:241642/150‑1 (MQ=255)
                                                                                                             cTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGAGCACGCTTTCATCAAAGGGGGCCCATa                                 >  4:165590/1‑150 (MQ=255)
                                                                                                              tttGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAc                                >  2:162850/1‑150 (MQ=255)
                                                                                                              tttGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAc                                >  1:710908/1‑150 (MQ=255)
                                                                                                              tttGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAc                                >  2:518930/1‑150 (MQ=255)
                                                                                                               ttGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAcc                               >  1:688540/1‑150 (MQ=255)
                                                                                                                tGATAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGATGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAgcc                              >  4:221234/1‑150 (MQ=255)
                                                                                                                  aTAATCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATAccccc                            <  3:322804/150‑1 (MQ=255)
                                                                                                                    aaTCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCgg                          >  1:600362/1‑150 (MQ=255)
                                                                                                                     aTCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGc                         >  2:688084/1‑150 (MQ=255)
                                                                                                                     aTCAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGc                         <  2:600362/150‑1 (MQ=255)
                                                                                                                       cAGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGt                       <  1:51728/150‑1 (MQ=255)
                                                                                                                        aGCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGtt                      <  2:287223/150‑1 (MQ=255)
                                                                                                                         gCGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTg                     <  2:278147/150‑1 (MQ=255)
                                                                                                                          cGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCAt                                  <  1:832437/136‑1 (MQ=255)
                                                                                                                          cGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCAt                                  >  2:832437/1‑136 (MQ=255)
                                                                                                                          cGAAATGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGa                    >  2:766240/1‑150 (MQ=255)
                                                                                                                             aaTGCAACGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATgg                 <  2:99259/150‑1 (MQ=255)
                                                                                                                                  aaCGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGa            <  2:710908/150‑1 (MQ=255)
                                                                                                                                    cGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGaac          >  4:457209/1‑150 (MQ=255)
                                                                                                                                    cGCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGaac          >  2:529847/1‑150 (MQ=255)
                                                                                                                                     gCAAGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGaaca         >  2:714108/1‑150 (MQ=255)
                                                                                                                                        aGCTTGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGaacaaaa      <  1:512759/150‑1 (MQ=255)
                                                                                                                                            tGCCATATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAAt  <  3:165590/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGAAAGCCCATTACATGGCGAAGGGAAGCAGTTTGAATGTGTTTGCGACGTGAATTAACTGACCTAACGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAAT  >  NC_000913_3_pae_tpiA/272736‑273025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: