Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,184,711 C→T T1184M (ACG→ATG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,184,7110CT98.7% 261.2 / ‑5.7 74T1184M (ACG→ATG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (37/36);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGC  >  NC_000913_3_pae_tpiA/4184564‑4184857
                                                                                                                                                   |                                                                                                                                                  
agcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGc                                                                                                                                                  <  3:276094/150‑1 (MQ=255)
  caAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCg                                                                                                                                                <  3:144791/150‑1 (MQ=255)
  caAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCg                                                                                                                                                >  1:699759/1‑150 (MQ=255)
       agTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGtt                                                                                                                                           >  4:209143/1‑150 (MQ=255)
        gTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTc                                                                                                                                          >  1:729161/1‑150 (MQ=255)
           gcgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGAc                                                                                                                                       >  2:204047/1‑150 (MQ=255)
            cgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACg                                                                                                                                      <  2:632638/150‑1 (MQ=255)
                    cgcgAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGaaa                                                                                                                              <  1:204047/150‑1 (MQ=255)
                    cgcgAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGaaa                                                                                                                              >  2:163316/1‑150 (MQ=255)
                    cgcgAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGaaa                                                                                                                              <  3:516179/150‑1 (MQ=255)
                                cAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAAtt                                                                                                                  <  2:24202/150‑1 (MQ=255)
                                        gtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAgct                                                                                                          >  1:155379/1‑150 (MQ=255)
                                            gATCGGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACATTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATGAAAGAGctgctg                                                                                                      >  2:705031/1‑150 (MQ=255)
                                              tCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGt                                                                                                                                    >  1:231002/1‑118 (MQ=255)
                                              tCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGt                                                                                                                                    <  2:231002/118‑1 (MQ=255)
                                                  ggCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACtt                                                                                                >  3:265576/1‑150 (MQ=255)
                                                    cgcTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGTGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTgg                                                                                              <  1:69717/150‑1 (MQ=255)
                                                     gcTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGc                                                                                             >  4:412290/1‑150 (MQ=255)
                                                       tGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGa                                                                                           >  2:134036/1‑150 (MQ=255)
                                                           gTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTg                                                                                       >  1:458377/1‑150 (MQ=255)
                                                             tCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGcc                                                                                     <  3:353487/150‑1 (MQ=255)
                                                             tCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGATGCTGAAACTTGGCGACCTGcc                                                                                     >  2:683982/1‑150 (MQ=255)
                                                               gTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGa                                                                                   >  4:136338/1‑150 (MQ=255)
                                                                tCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGAc                                                                                  >  2:637972/1‑150 (MQ=255)
                                                                   gAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                               >  4:157995/1‑150 (MQ=255)
                                                                   gAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                               >  1:303300/1‑150 (MQ=255)
                                                                     aaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCg                                                                             <  3:136338/150‑1 (MQ=255)
                                                                      aGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTTCTGAAACTTGGCGACCTGCCGACTTCCgg                                                                            >  4:487099/1‑150 (MQ=255)
                                                                      aGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCgg                                                                            <  1:637972/150‑1 (MQ=255)
                                                                       gTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGt                                                                           <  1:304334/150‑1 (MQ=255)
                                                                        ttGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                          <  2:395011/150‑1 (MQ=255)
                                                                        ttGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                          <  3:32342/150‑1 (MQ=255)
                                                                          gACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAg                                                                        <  1:134036/150‑1 (MQ=255)
                                                                               gAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCg                                                                   >  3:246123/1‑150 (MQ=255)
                                                                                 gTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                 <  1:202860/150‑1 (MQ=255)
                                                                                     cTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTa                                                             <  2:729161/150‑1 (MQ=255)
                                                                                     cTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTa                                                             <  4:440890/150‑1 (MQ=255)
                                                                                     cTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTa                                                             <  1:267407/150‑1 (MQ=255)
                                                                                       tCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACg                                                           >  1:519412/1‑150 (MQ=255)
                                                                                          gCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCg                                                                                    <  2:253161/122‑1 (MQ=255)
                                                                                          gCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCg                                                                                    >  1:253161/1‑122 (MQ=255)
                                                                                           cGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                               <  2:754334/126‑1 (MQ=255)
                                                                                           cGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                               >  1:754334/1‑126 (MQ=255)
                                                                                                      ttATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTg                                            >  1:351652/1‑150 (MQ=255)
                                                                                                         tGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGAACTgttcacc                                         >  3:197415/1‑144 (MQ=255)
                                                                                                          gCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACa                                        <  2:612886/150‑1 (MQ=255)
                                                                                                            gTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGt                                      >  1:572702/1‑150 (MQ=255)
                                                                                                                ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCTAAAGAAGCTGAAAGTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGa                                  <  1:705031/150‑1 (MQ=255)
                                                                                                                ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGATCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGa                                  <  2:749034/150‑1 (MQ=255)
                                                                                                                ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCACCTGTACGATGGTCGCACTGGTGAACAGTTCGa                                  >  2:290070/1‑150 (MQ=255)
                                                                                                                 gCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                 >  3:228068/1‑150 (MQ=255)
                                                                                                                     aaaaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAgctgc                                                                                                        <  1:568134/75‑1 (MQ=255)
                                                                                                                     aaaaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAgctgc                                                                                                        >  2:568134/1‑75 (MQ=255)
                                                                                                                     aaaaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTc                             <  4:265576/150‑1 (MQ=255)
                                                                                                                     aaaaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTc                             <  2:259013/150‑1 (MQ=255)
                                                                                                                       aaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTg                                                                                               >  1:508238/1‑82 (MQ=255)
                                                                                                                       aaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTg                                                                                               <  2:508238/82‑1 (MQ=255)
                                                                                                                       aaCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCg                           <  3:209143/150‑1 (MQ=255)
                                                                                                                        aCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCgg                          >  1:117341/1‑150 (MQ=255)
                                                                                                                        aCCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCgg                          >  1:117368/1‑150 (MQ=255)
                                                                                                                            gcgcAAAGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAAc                      >  3:485280/1‑150 (MQ=255)
                                                                                                                                aaaGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGtt                  >  3:309154/1‑150 (MQ=255)
                                                                                                                                 aaGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTg                 <  4:228068/150‑1 (MQ=255)
                                                                                                                                 aaGGTATGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTg                 <  2:572702/150‑1 (MQ=255)
                                                                                                                                   ggTATGCCAATCGCAATGCCGGTGCTCGACGGTGCCAAAGTTGCATAATTTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGt               <  3:232349/150‑1 (MQ=255)
                                                                                                                                      aTGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtac            <  2:458377/150‑1 (MQ=255)
                                                                                                                                      aTGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtac            <  1:64019/150‑1 (MQ=255)
                                                                                                                                       tGCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtaca           >  3:386889/1‑150 (MQ=255)
                                                                                                                                        gCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacat          >  3:138023/1‑150 (MQ=255)
                                                                                                                                        gCCAATCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacat          <  2:699759/150‑1 (MQ=255)
                                                                                                                                          cagtCGCAATGCCGGTATTCGACAGAACGAAATAAGCAGTACAACAAGAGGTGCTGAAACTAGACTAGATGACGACTTCCGGACAGATCCGACTGGACGATGGTCGCCCTGGTGACCAGGTCGAGCGTCCGGTAACCGCTGGTtacatgt        >  2:819983/4‑150 (MQ=255)
                                                                                                                                           aaTCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTc                                                     <  3:141657/104‑1 (MQ=255)
                                                                                                                                           aaTCGCAATGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTc                                                     >  4:141657/1‑104 (MQ=255)
                                                                                                                                                aaaCGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGc  <  4:19529/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGC  >  NC_000913_3_pae_tpiA/4184564‑4184857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑