Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,619,320	G→A	Q392Q (CAG→CAA)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,619,320	0	G	A	100.0%	305.4 / NA	83	Q392Q (CAG→CAA)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/39);  total (44/39)

TGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATA  >  NC_000913_3_pae_tpiA/4619176‑4619466                                                                                                                                                 |                                                                                                                                                   tGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGa                                                                                                                                               <  2:684538/150‑1 (MQ=255)  gCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGAc                                                                                                                                              <  1:782897/150‑1 (MQ=255)                 tttGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTg                                                                                                                               >  3:240292/1‑150 (MQ=255)                    gTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCg                                                                                                                            >  2:542453/1‑150 (MQ=255)                    gTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATACGGCGCGTCTGGGCGACCAAGTAGACGGTCACCGTCCGCTGGCg                                                                                                                            >  2:343797/1‑150 (MQ=255)                     tCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGCCGCAGTCAGGAATGTGACACGATCGATTCCAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCgg                                                                                                                           >  2:36835/1‑150 (MQ=255)                       aGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGtt                                                                                                                         <  2:622882/150‑1 (MQ=255)                         tGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGa                                                                                                                                               >  3:509014/1‑126 (MQ=255)                         tGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGa                                                                                                                                               <  4:509014/126‑1 (MQ=255)                         tGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTAt                                                                                                                       >  3:164497/1‑150 (MQ=255)                           aaaTGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGGCTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATcc                                                                                                                     >  3:273180/1‑150 (MQ=255)                             aTGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAc                                                                                                                   <  1:150738/150‑1 (MQ=255)                               ggATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcgc                                                                                                                 <  1:373334/150‑1 (MQ=255)                                gATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcgcg                                                                                                                >  4:295107/1‑150 (MQ=255)                                gATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcgcg                                                                                                                >  1:17071/1‑150 (MQ=255)                                   aCCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGaaa                                                                                                             >  1:5480/1‑150 (MQ=255)                                   aCCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGaaa                                                                                                             <  4:3783/150‑1 (MQ=255)                                     ccGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcg                                                                                                                  <  3:333024/143‑1 (MQ=255)                                     ccGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcg                                                                                                                  >  4:333024/1‑143 (MQ=255)                                     ccGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGa                                                                                                           <  4:335641/150‑1 (MQ=255)                                       gcgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACg                                                                                                         >  3:337018/1‑150 (MQ=255)                                        cgcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGGGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGa                                                                                                        <  1:479900/150‑1 (MQ=255)                                           gcTGGGGATGGCCGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGaaaa                                                                                                     >  2:471436/1‑150 (MQ=255)                                            cTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGGGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAaac                                                                                                    <  1:542453/150‑1 (MQ=255)                                               gggATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAaacaac                                                                                                 <  1:123766/150‑1 (MQ=255)                                               gggATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAaacaac                                                                                                 >  2:488844/1‑150 (MQ=255)                                                    ggCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCa                                                                                            <  1:762086/150‑1 (MQ=255)                                                    ggCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCa                                                                                            <  4:133351/150‑1 (MQ=255)                                                        gTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGaa                                                                                        >  2:260637/1‑150 (MQ=255)                                                         tGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAg                                                                                       >  3:247274/1‑150 (MQ=255)                                                            ttGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAgcgg                                                                                    <  4:337018/150‑1 (MQ=255)                                                                  tGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAg                                                                              <  3:508476/150‑1 (MQ=255)                                                                   gggCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGtt                                                                                                                         >  2:303862/1‑106 (MQ=255)                                                                   gggCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGtt                                                                                                                         <  1:303862/106‑1 (MQ=255)                                                                      cggcggACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGt                                                                          <  1:438037/150‑1 (MQ=255)                                                                       ggcggACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTg                                                                         >  2:768370/1‑150 (MQ=255)                                                                              gCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCgg                                                                  >  1:780035/1‑150 (MQ=255)                                                                                 gTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAaac                                                                                                    >  3:185729/1‑113 (MQ=255)                                                                                 gTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAaac                                                                                                    <  4:185729/113‑1 (MQ=255)                                                                                 gTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCaa                                                               >  3:438157/1‑150 (MQ=255)                                                                                  tCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAAt                                                              >  1:230811/1‑150 (MQ=255)                                                                                    aGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCa                                                                                            >  1:743795/1‑118 (MQ=255)                                                                                    aGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCa                                                                                            <  2:743795/118‑1 (MQ=255)                                                                                      gCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTaaa                                                          >  1:507004/1‑150 (MQ=255)                                                                                        aTCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACt                                                        >  1:559057/1‑150 (MQ=255)                                                                                           tGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTCTCCCCACGAAAGACGCGAAAAAATGGTAGGGAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGc                                                     >  3:75849/1‑150 (MQ=255)                                                                                           tGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGc                                                     <  1:660145/150‑1 (MQ=255)                                                                                           tGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGc                                                     <  2:676924/150‑1 (MQ=255)                                                                                           tGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGc                                                     <  2:368997/150‑1 (MQ=255)                                                                                           tGACACCATCGATGACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGc                                                     <  2:494113/150‑1 (MQ=255)                                                                                            gACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcg                                                                                                                  <  4:291280/88‑1 (MQ=255)                                                                                            gACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCAcg                                                                                                                  >  3:291280/1‑88 (MQ=255)                                                                                              cacCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGa                                                  >  1:273141/1‑150 (MQ=255)                                                                                               acCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTaaa                                                          <  2:731630/141‑1 (MQ=255)                                                                                               acCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTaaa                                                          >  1:731630/1‑141 (MQ=255)                                                                                                  aTCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATaaa                                              >  1:673247/1‑150 (MQ=255)                                                                                                       ttACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCAcc                                         >  3:32223/1‑150 (MQ=255)                                                                                                             cGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAg                                   <  4:427316/150‑1 (MQ=255)                                                                                                             cGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAg                                   <  2:736396/150‑1 (MQ=255)                                                                                                              gTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGc                                  <  4:438157/150‑1 (MQ=255)                                                                                                               tCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGca                                 <  1:356635/150‑1 (MQ=255)                                                                                                               tCGGCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGca                                 >  4:324935/1‑150 (MQ=255)                                                                                                                  gCTTTACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCacac                              <  1:321519/150‑1 (MQ=255)                                                                                                                    tttACTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCa                            >  3:13233/1‑150 (MQ=255)                                                                                                                       aCTGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACt                         >  3:509887/1‑150 (MQ=255)                                                                                                                         tGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGt                       <  3:295107/150‑1 (MQ=255)                                                                                                                         tGATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGt                       >  4:177533/1‑150 (MQ=255)                                                                                                                         ggATATGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGt                       <  2:717440/149‑1 (MQ=255)                                                                                                                           atatGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTca                     >  3:208935/1‑149 (MQ=255)                                                                                                                           atatGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCt                     >  1:9960/1‑150 (MQ=255)                                                                                                                              tGGCGCGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATc                  <  4:164497/150‑1 (MQ=255)                                                                                                                                gcgcGTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGc                >  2:735412/1‑150 (MQ=255)                                                                                                                                  gcgTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGcc               <  2:734053/149‑1 (MQ=255)                                                                                                                                  gcgTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGcc               >  1:734053/1‑149 (MQ=255)                                                                                                                                    gTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAgcgg                                                                                    <  2:403058/78‑1 (MQ=255)                                                                                                                                    gTCTGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAgcgg                                                                                    >  1:403058/1‑78 (MQ=255)                                                                                                                                       tGGGCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCa         >  2:184182/1‑150 (MQ=255)                                                                                                                                         ggCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGc       <  4:509887/150‑1 (MQ=255)                                                                                                                                          gCGACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCg      <  3:177533/150‑1 (MQ=255)                                                                                                                                            gACCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTTAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGaa    >  2:21049/1‑150 (MQ=255)                                                                                                                                             aCCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGa                                                                        <  2:752157/81‑1 (MQ=255)                                                                                                                                             aCCAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGa                                                                        >  1:752157/1‑81 (MQ=255)                                                                                                                                              ccAAGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATa  >  4:140802/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATA  >  NC_000913_3_pae_tpiA/4619176‑4619466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑