Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,220,766 C→T pseudogene (1166/2574 nt) ycgH → pseudogene; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,220,7660CT98.6% 236.9 / NA 69pseudogene (1166/2574 nt)ycgHpseudogene; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (32/36);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACTACAACGGATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAACCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAG  >  NC_000913_3_pae_tpiA/1220621‑1220909
                                                                                                                                                 |                                                                                                                                               
aaaaCTACAACGGATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTGAATGGAGCGACTAAACTCGTCAGCGGCACAATCACg                                                                                                                                             <  4:195026/150‑1 (MQ=255)
         aCGGATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCtt                                                                                                                                    >  2:83834/1‑150 (MQ=255)
         aCGGATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCtt                                                                                                                                    >  1:625374/1‑150 (MQ=255)
          cGGATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCttt                                                                                                                                   <  1:162591/150‑1 (MQ=255)
           ggATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTc                                                                                                                                  <  4:437209/150‑1 (MQ=255)
               gTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAAATCGTCAGCGGCACAATCACGCTGAAGATTTCAGaa                                                                                                                              >  1:639366/1‑150 (MQ=255)
                tAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGaaa                                                                                                                             <  1:130393/150‑1 (MQ=255)
                tAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGaaa                                                                                                                             <  1:236141/150‑1 (MQ=255)
                 aaGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGaaaa                                                                                                                            <  4:46347/150‑1 (MQ=255)
                       ccTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAAt                                                                                                                      <  2:678283/150‑1 (MQ=255)
                        cTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATc                                                                                                                     >  2:778674/1‑150 (MQ=255)
                         ttAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCt                                                                                                                    >  3:224904/1‑150 (MQ=255)
                                    attatCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGaaa                                                                                                         >  4:518037/1‑150 (MQ=255)
                                    attatCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGaaa                                                                                                         <  1:548826/150‑1 (MQ=255)
                                       atCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGAt                                                                                                      <  1:531471/150‑1 (MQ=255)
                                        tCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCt                                                                                                                                           >  3:331196/1‑112 (MQ=255)
                                        tCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCt                                                                                                                                           <  4:331196/112‑1 (MQ=255)
                                           cacCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACt                                                                                                  >  3:30678/1‑150 (MQ=255)
                                                         gTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCAt                                                                                    <  4:30678/150‑1 (MQ=255)
                                                           tCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCt                                                                                  <  2:499972/150‑1 (MQ=255)
                                                                  aataatACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAAt                                                                           <  1:665144/150‑1 (MQ=255)
                                                                   ataataCTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAAtt                                                                          <  4:224904/150‑1 (MQ=255)
                                                                    taataCTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTc                                                                         <  3:114258/150‑1 (MQ=255)
                                                                     aataCTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCa                                                                        >  1:284300/1‑150 (MQ=255)
                                                                     aataCTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCa                                                                        >  4:291385/1‑150 (MQ=255)
                                                                       taCTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGa                                                                      <  1:83834/150‑1 (MQ=255)
                                                                          tGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGa                                                                                                           >  3:213930/1‑110 (MQ=255)
                                                                          tGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGa                                                                                                           <  4:213930/110‑1 (MQ=255)
                                                                          tGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAg                                                                   >  2:202657/1‑150 (MQ=255)
                                                                          tGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAg                                                                   >  1:115029/1‑150 (MQ=255)
                                                                            gAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGta                                                                 <  2:742593/150‑1 (MQ=255)
                                                                               gTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGtatta                                                              <  3:131906/150‑1 (MQ=255)
                                                                                tGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGtattat                                                             <  3:291385/150‑1 (MQ=255)
                                                                                    ttAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAAt                                                         >  3:368592/1‑150 (MQ=255)
                                                                                    ttAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAAt                                                         >  2:417734/1‑150 (MQ=255)
                                                                                      aaaCATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCt                                                       >  2:583473/1‑150 (MQ=255)
                                                                                         cATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTc                                                    >  3:234683/1‑150 (MQ=255)
                                                                                          aTAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCg                                                   <  4:234683/150‑1 (MQ=255)
                                                                                              tttCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTatg                                               <  3:518037/150‑1 (MQ=255)
                                                                                              tttCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTatg                                               >  4:275199/1‑150 (MQ=255)
                                                                                               ttCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTatga                                              <  1:778674/150‑1 (MQ=255)
                                                                                                  gTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTatgatga                                           >  1:736406/1‑150 (MQ=255)
                                                                                                   tCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTatgatgat                                          <  3:18306/150‑1 (MQ=255)
                                                                                                       aCTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTc                                      <  1:202657/150‑1 (MQ=255)
                                                                                                       aCTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTc                                      <  4:155722/150‑1 (MQ=255)
                                                                                                            aTTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCt                                                                                  <  1:699004/101‑1 (MQ=255)
                                                                                                            aTTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCt                                                                                  >  2:699004/1‑101 (MQ=255)
                                                                                                            aTTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACa                                 >  3:191742/1‑150 (MQ=255)
                                                                                                                tAAATGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACAtttg                             >  1:794838/1‑149 (MQ=255)
                                                                                                                 aaaTGGAGCGACTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTAc                            >  1:459533/1‑150 (MQ=255)
                                                                                                                           aCTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCaa                        >  2:524398/1‑144 (MQ=255)
                                                                                                                           aCTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCaa                        <  1:524398/144‑1 (MQ=255)
                                                                                                                           aCTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGaaaa                  >  1:58981/1‑150 (MQ=255)
                                                                                                                            cTAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTGCCCAAGGaaaaa                 <  2:794838/150‑1 (MQ=255)
                                                                                                                             tAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAAc                >  2:675075/1‑150 (MQ=255)
                                                                                                                             tAAACTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAAc                >  2:675183/1‑150 (MQ=255)
                                                                                                                              aaaCTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACa               <  3:239825/150‑1 (MQ=255)
                                                                                                                               aaCTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACAt              >  1:721507/1‑150 (MQ=255)
                                                                                                                               aaCTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACAt              <  1:277282/150‑1 (MQ=255)
                                                                                                                                aCTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTAc                            <  2:548302/135‑1 (MQ=255)
                                                                                                                                aCTCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTAc                            >  1:548302/1‑135 (MQ=255)
                                                                                                                                  tCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACAt                                >  2:344413/1‑129 (MQ=255)
                                                                                                                                  tCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACAt                                <  1:344413/129‑1 (MQ=255)
                                                                                                                                  tCGTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTaa           >  4:141985/1‑150 (MQ=255)
                                                                                                                                    gTCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACCAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAAcc         <  1:802707/150‑1 (MQ=255)
                                                                                                                                    gTCAGCGGCACAACCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTTCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAAcc         <  1:200854/150‑1 (MQ=255)
                                                                                                                                     tCAGCGGCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCg        >  3:184828/1‑150 (MQ=255)
                                                                                                                                          ggCACAATCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTaaaa   <  1:127987/150‑1 (MQ=255)
                                                                                                                                           gCACAATCACACTGAAGCTTTCAGAAAAAACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACAAAAAAAAAAAAAAAAACATTAACCGTAAAAg  <  2:625374/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAAACTACAACGGATGTAAGTATCCTTAATCGAAGTATTATCACACCTGGTAATAATGTTCTGGTTAATAATACTGGAGGTGACTTAAACATAATTTCGTCCGACTCTATTCTAAATGGAGCGACTAAACTCGTCAGCGGCACAACCACGCTGAAGCTTTCAGAAAATACAATCTGGAATATGAAAGATGACTCCGTTGTTACCCATCTGACTAATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAG  >  NC_000913_3_pae_tpiA/1220621‑1220909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑