Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,747 G→C intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,7470GC95.0% 409.1 / 11.8 120intergenic (+62/+784)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base C (63/51);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 122 total aligned reads displayed.

TGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCC  >  NC_000913_3_pae_tpiA/4110603‑4110889
                                                                                                                                                |                                                                                                                                              
     acacAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGGGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCcgccgc                                                                                                                                      <  2:676670/150‑1 (MQ=255)
       tcgaCGAGATCTAGCGGCGCTTAACCGTGCTTCCGCCGAGAAAATCAAGGATCATCAGTGTGACATTTCGCCGTCAGGCGAAGCGGTAAGGAAAGTATGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGGCCCGCcccgc                                                                                                                                    >  2:731516/4‑149 (MQ=255)
       acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                    <  4:496323/150‑1 (MQ=255)
       acaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGa                                                                                                                                    >  2:145346/1‑150 (MQ=255)
         aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAggcggc                                                                                                                                              <  2:63297/138‑1 (MQ=255)
         aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAggcggc                                                                                                                                              >  1:63297/1‑138 (MQ=255)
         aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGc                                                                                                                                  >  2:839335/1‑150 (MQ=255)
          aCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCg                                                                                                                                 <  3:569048/150‑1 (MQ=255)
               aTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTc                                                                                                                            <  1:103545/150‑1 (MQ=255)
                  tACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGcc                                                                                                                         >  2:499264/1‑150 (MQ=255)
                     tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAt                                                                                                                      >  2:288137/1‑150 (MQ=255)
                         gCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTa                                                                                                                  >  3:231900/1‑150 (MQ=255)
                         gCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTa                                                                                                                  <  2:672186/150‑1 (MQ=255)
                             aaCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGTTATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGAt                                                                                                              <  1:457020/150‑1 (MQ=255)
                                cGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATc                                                                                                             <  2:45409/148‑1 (MQ=255)
                                cGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATc                                                                                                             >  1:45409/1‑148 (MQ=255)
                                 gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTa                                                                                                          <  4:231900/150‑1 (MQ=255)
                                  tGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                         <  1:842787/150‑1 (MQ=255)
                                    cTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGa                                                                                                       <  3:462317/150‑1 (MQ=255)
                                     ttCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGat                                                                                                      >  4:420692/1‑150 (MQ=255)
                                        aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGGCTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAAt                                                                                                                      <  1:60474/131‑1 (MQ=255)
                                        aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATg                                                                                                   >  3:390231/1‑150 (MQ=255)
                                         gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  <  3:172830/150‑1 (MQ=255)
                                         gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  <  4:198919/150‑1 (MQ=255)
                                         gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  <  3:509648/150‑1 (MQ=255)
                                          ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                       >  1:822774/1‑128 (MQ=255)
                                          ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCaa                                                                                                                       <  2:822774/128‑1 (MQ=255)
                                          ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATggg                                                                                                 >  3:302131/1‑150 (MQ=255)
                                          ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  <  1:743047/149‑1 (MQ=255)
                                          ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  >  2:743047/1‑149 (MQ=255)
                                            gAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTc                                                                                               >  3:496266/1‑150 (MQ=255)
                                                  aTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGc                                                                                         >  3:385360/1‑150 (MQ=255)
                                                    tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  >  1:371885/1‑139 (MQ=255)
                                                    tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATgg                                                                                                  <  2:371885/139‑1 (MQ=255)
                                                           catcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCGATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGc                                                                                >  3:533004/1‑150 (MQ=255)
                                                            atcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCt                                                                               <  2:917749/150‑1 (MQ=255)
                                                            atcaGTGTGAGATCTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCt                                                                               <  2:837037/150‑1 (MQ=255)
                                                             tcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTg                                                                              >  1:216428/1‑150 (MQ=255)
                                                                gtgtGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgcgc                                                                           <  2:742376/150‑1 (MQ=255)
                                                                 tgtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCt                                                                          >  3:494298/1‑150 (MQ=255)
                                                                   tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                        >  1:662936/1‑150 (MQ=255)
                                                                   tgAGATTTTGCGTTAAGGCGAAGAGTCCAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGGCTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTg                                                                        >  4:487946/1‑150 (MQ=255)
                                                                      gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                         <  1:931186/114‑1 (MQ=255)
                                                                      gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTaa                                                                                                         >  2:931186/1‑114 (MQ=255)
                                                                      gaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCa                                                                     <  2:954133/150‑1 (MQ=255)
                                                                       aTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAg                                                                    <  1:790476/150‑1 (MQ=255)
                                                                       aTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAg                                                                    <  2:831554/150‑1 (MQ=255)
                                                                        ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGccc                                                                                                                                            >  2:838679/1‑77 (MQ=255)
                                                                                  aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACAcccccc                                                         >  2:891647/1‑150 (MQ=255)
                                                                                  aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTcccc                                                         >  1:779906/1‑150 (MQ=255)
                                                                                   ggCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCt                                                        <  1:839335/150‑1 (MQ=255)
                                                                                            ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCtt                                                                                                           <  1:807809/90‑1 (MQ=255)
                                                                                            atAAGGAAAGTAAGTGCCGGATCTGCAACCCGGCACCGGTCAGACTGAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCGTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAg                                               >  2:395616/2‑150 (MQ=255)
                                                                                             tAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGt                                              <  2:398526/150‑1 (MQ=255)
                                                                                              aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgc                                                                             >  3:122186/1‑118 (MQ=255)
                                                                                              aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTgc                                                                             <  4:122186/118‑1 (MQ=255)
                                                                                               aGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGttt                                            <  1:949100/150‑1 (MQ=255)
                                                                                                 gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTaa                                          >  2:548498/1‑150 (MQ=255)
                                                                                                     gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAgcgc                                      >  1:142349/1‑150 (MQ=255)
                                                                                                      taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                     <  3:226816/150‑1 (MQ=255)
                                                                                                      taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGATTGACAGAAACACTGCCCTCGTGCCGATTTTAAGcacg                                     <  2:305818/150‑1 (MQ=255)
                                                                                                       aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCt                                                                                              <  1:407989/92‑1 (MQ=255)
                                                                                                       aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCt                                                                                              >  2:407989/1‑92 (MQ=255)
                                                                                                       aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCgg                                    <  3:300117/150‑1 (MQ=255)
                                                                                                         gtGCCGGATTTCATATCCGGCACTTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                         >  4:568175/1‑111 (MQ=255)
                                                                                                         gtGCCGGATTTCATATCCGGCACTTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCtt                                                                         <  3:568175/111‑1 (MQ=255)
                                                                                                         gtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCt                                  >  2:886318/1‑150 (MQ=255)
                                                                                                          tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                 >  4:316112/1‑150 (MQ=255)
                                                                                                          tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                 <  1:288137/150‑1 (MQ=255)
                                                                                                              ggATATGTAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                             <  1:748663/150‑1 (MQ=255)
                                                                                                              ggATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                     >  2:847882/1‑142 (MQ=255)
                                                                                                              ggATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGcgcg                                     <  1:847882/142‑1 (MQ=255)
                                                                                                               gATATGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACg                            >  2:699993/1‑150 (MQ=255)
                                                                                                                 tatGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                          >  3:538096/1‑150 (MQ=255)
                                                                                                                 tatGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                          <  2:306467/150‑1 (MQ=255)
                                                                                                                 tatGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                          <  4:385360/150‑1 (MQ=255)
                                                                                                                  atGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTcc                         >  2:441362/1‑150 (MQ=255)
                                                                                                                   tGAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGatat                                                                                                    >  2:435011/1‑74 (MQ=255)
                                                                                                                    gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                       >  3:253794/1‑150 (MQ=255)
                                                                                                                    gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                       >  3:384208/1‑150 (MQ=255)
                                                                                                                    gAAATCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctc                       >  1:893402/1‑150 (MQ=255)
                                                                                                                     aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTACGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTc                                                       <  2:457781/117‑1 (MQ=255)
                                                                                                                     aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGc                                                   >  3:11063/1‑121 (MQ=255)
                                                                                                                     aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGc                                                   <  4:11063/121‑1 (MQ=255)
                                                                                                                     aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                      >  1:237112/1‑150 (MQ=255)
                                                                                                                     aaaTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCGTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTc                                                       >  1:457781/1‑117 (MQ=255)
                                                                                                                       aTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCCAACCTACGCTCCTACAGAGTTTAAGCGCTGCTGCAACGTCCTCTcc                    >  1:902455/1‑150 (MQ=255)
                                                                                                                       aTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                 >  1:491953/1‑137 (MQ=255)
                                                                                                                       aTCCGGCACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCtt                                 <  2:491953/137‑1 (MQ=255)
                                                                                                                             cACCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCtctc              <  1:145346/150‑1 (MQ=255)
                                                                                                                              aCCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAacac                                                              <  3:542024/101‑1 (MQ=255)
                                                                                                                              aCCTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAacac                                                              >  4:542024/1‑101 (MQ=255)
                                                                                                                               ccTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGc                                                                                         >  1:395863/1‑73 (MQ=255)
                                                                                                                                cTGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAt           <  4:384208/150‑1 (MQ=255)
                                                                                                                                 tGTCAGACTTAAGGCCGCCCCGCCGCGAGCGGTCTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCCGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGGTTAAGCGCGGCTGCAACGTCCTCTCCGCTCCAAATa          >  4:522593/1‑150 (MQ=255)
                                                                                                                                   tCAGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAcc        >  2:807334/1‑150 (MQ=255)
                                                                                                                                     aGACTTAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCg      <  1:925611/150‑1 (MQ=255)
                                                                                                                                         ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcccc  >  2:732220/1‑150 (MQ=255)
                                                                                                                                         ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcccc  >  1:311505/1‑150 (MQ=255)
                                                                                                                                         ttAAGGCCGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGAAACAATCAAATAGAGCAAAGTTTAAGCGCGGCTTCAAAGTCCTCTCCGCTCTCAATACCCGcccc  >  3:235062/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCC  >  NC_000913_3_pae_tpiA/4110603‑4110889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: