| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 2,817,170 | C→A | E112* (GAA→TAA) | yqaB ← | fructose‑1‑P and 6‑phosphogluconate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 2,817,170 | 0 | C | A | 94.4% | 34.6 / NA | 18 | E112* (GAA→TAA) | yqaB | fructose‑1‑P and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (7/10); minor base G (1/0); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC > NC_000913_3_pae_tpiA/2817033‑2817314 | cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTc < 1:316390/149‑1 (MQ=255) aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCTCGGGGT‑TATAGTGTCTGACGTCATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCCGCGATGGCGCCTTGACTCCCCGCTcc > 2:91157/1‑149 (MQ=255) aCGCCCATACGCTGCGCGCACAACAAAAATGTGGCTGGCGCGGGAT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCATAAACCCAGAGGCGCCAGCCATGCCTCAGCGCTGGCGCTTTAACTCCCCGttac > 2:88636/1‑147 (MQ=255) gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTa < 2:283050/149‑1 (MQ=255) ccATACGCTGCGCGCACAACAAAAATGTGCCTCG‑GCAGTTTGTATGGTGTTTGACGTGATCGGCAGCAACGGCGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCGCAGCAGCAGCGCTCTAACTCCCCGTTCCTAcg > 1:102026/1‑148 (MQ=255) aacaaAAATGTGTCTGGCGCGGGTT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTCCAGCCATTGGGCGACGAcc > 1:333073/1‑149 (MQ=255) aacaaAAAGGTGTCTGGCGCGGGTT‑TATGGTGTGTGACGTGATCGGCAGCGACGACGGCGTCTAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGTGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGAcc < 2:374351/149‑1 (MQ=255) cTGGCGCGGGTT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTCCCTACAGCCATTGGGCGACGACCATGCCAACTCTTc > 1:256930/1‑149 (MQ=255) t‑tATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaac < 2:220848/149‑1 (MQ=255) gtacggTGTTTGACGTGATCTGGAGCGACGACTGCGTCAAAATAATGGCCTAATCCCAGGTGCGCCAGCAATGCCTCAGCGAGGGCGCTTTATCTCCCCGTTCCTACAGCCATTGTGCGACGACCATGCCAACTTTTCACCACATcagc < 2:287826/145‑3 (MQ=255) ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga < 1:255333/149‑1 (MQ=255) ccGGCAGCGAGGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCCCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACCTTTCACCACATCAACAAGAGGAAGCGGTTCGAc < 1:343239/148‑1 (MQ=255) aTCCCAGGTGCGACAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGtt < 1:176792/149‑1 (MQ=255) ggTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCa < 1:367019/149‑1 (MQ=255) cAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACCCGCTa > 2:229111/1‑149 (MQ=255) gcTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGGCGAGATCgg > 1:420397/1‑149 (MQ=255) cTTTAACTCCCCGTTCCTACAGACATTGGGCGACGACCATGCCAACTTTTTACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc < 2:264246/149‑1 (MQ=255) tttAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACAt > 1:130881/1‑57 (MQ=255) | CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTT‑TATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGC > NC_000913_3_pae_tpiA/2817033‑2817314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |